Indonesian Journal of Animal and Veterinary Sciences

90 - 95

Authors: Fera Mahmilia

Eichhornia crassipes Mart is one of the water plants which grows in the rivers, ricefields, water reservoirs or dams. This plant is often considered as water weeds that harm people much. This weed can be used for feeding the animal, but it has high crude fiber. Fermentation tehnology could be done to overcome the problem. E. crassipes Mart is grounded into meal and solidly fermented by mixing minerals and Trichoderma harzianum for 4 days at room temperature. The fermentation resulted the increase of nutritional value. The crude protein increases for 61.81% (from 6.31 to 10.21%) and crude fiber decreases for 18% (26.61 to 21.82%). The in vivo experiment was conducted based on completely randomized design using 80 day old broiler chicks with 5 replication. They were alotted to 4 diets containing one control without fermented E. crassipes Mart, 5, 10 and 15% of fermented E. crassipes Mart. Diets were fed ad libitum for 6 weeks. Feed intake, weight gain and feed conversion ratio, carcas and abdominal fat were observed as parameters. Result showed that no parameter were significantly affected by treatments, although the nutritional values were slightly decreasing in higher fermented E. crassipes. The fermented E. Crassipes Mart can be used up to 15% in broiler rations. Key Words: Eichhornia crassipes Mart Meal, Nutritional Value, Fermentation, Ration

106 - 112

Authors: T Kostaman, I-K Sutama

An experiment was conducted to study the growth patterns of Boer x Peranakan Etawah (PE) crossbred during pre-weaning period, at the Indonesian Research Institute for Animal Production, Ciawi-Bogor. Sixty-one kids were used in this experiment. They were reared with their mothers in group pen (3 x 4 m), with each pen contained 6-7 does. Does were fed of 2.5 kg freshlychopped King Grass and 0.7 kg concentrate head-1 day-1. Research results showed that birth weight of Boer x PE kids (group A) were significantly higher than those of PE kids (group B) (4.29 ± 0.63 vs 3.71 ± 0.89 kg/head, P<0.05). However, average preweaning daily weight gain (ADG) (116.40 ± 49.95 vs 105.29 ± 28.36 g/head, P>0.05) and weaning weight (14.64 ± 4.56 vs 13.30 ± 2.71 kg/head, P>0.05) were not significantly different between the groups. Pre-weaning mortality of the kids was relatively high in both group A (25%) and B (21,21%). While sex ratio (male : female) was 57.14 : 42.86% in group A and 51,52 : 48,48% in group B. Key Words: Growth, PE, Boer Goat, Pre-Weaning

118 - 126

Authors: Simson Tarigan

Vaccines developed from certain membrane proteins lining the lumen of arthropod’s gut have been demonstrated effective in the control of some arthropod ectoparasites. A similar approach could also be applied to Sarcoptes scabiei since this parasite also ingests its host immunoglobulins. To evaluate immune protection of the membrane proteins, insoluble mite proteins were fractionated by successive treatment in the solutions of 1.14 M NaCl, 2% SB 3-14 Zwitterion detergent, 6 M urea, 6 M guanidine-HCl and 5% SDS. Five groups of goats (6 or 7 goats per group) were immunised respectively with the protein fractions. Vaccination was performed 6 times, each with a dosage of 250 μg proteins, and 3 week intervals between vaccination. Group 6 (7 goats) received PBS and adjuvant only, and served as an unvaccinated control. One week after the last vaccination, all goats were challenged with 2000 live mites on the auricles. The development of lesions were examined at 1 day, 2 days, and then every week from week 1 to 8. All animals were bled and weighed every week, and at the end of the experiment, skin scrapings were collected to determine the mite burden. Antibody responses induced by vaccination and challenge were examined by ELISA and Western blotting. This experiment showed that vaccination with the insoluble-protein fractions resulted in the development of high level of specific antibodies but the responses did not have any protective value. The severity of lesions and mite burden in the vaccinated animals were not different from those in the unvaccinated control. Key Words: Sarcoptes scabiei, Insoluble Protein, Goat, Vaccination

134 - 142

Authors: April H Wardhana, Amir Husein, J Manurung

Boophilus microplus is the most important pest in livestock industries. The use of synthetic chemical acaricides is the main method of tick control, however, chemical acaricides are expensive, and they are harmful to environment and cause strain resistance. The aim of study was to investigate the affectivity of Annona squamosa L seeds extracted by diverse organic solvents such as water, methanol and hexane against mortality of Boophilus microplus larvae in vitro. Five hundred and fifty larvae were used in this study and divided into three groups e.g. water (3, 4 and 5% concentration), methanol and hexane extract groups (0.25, 0.50, and 0.75% concentration). Coumaphos (0.50%) was used as a positive control. The larvae were dipped into extract solution for 10 seconds and dried using filter paper. Their mortality was observed from one to five hours. The mortality data were transformed to Abbot formula and analyzed using probit analysis with 95% significant level. This study showed that the active compound of Annona squamosa L seeds had effectively contact toxic property for B. microplus larvae at 5, 0.50, and 0.75% for water, methanol and hexane extractions, respectively. The lethal concentrations of methanol extract (LC50, LC90, and LC95) were lower than hexane extract e.g. 0.32, 0.86, and 1.13%, respectively and for hexane extract were 0.35, 1.11, and 1.54%, respectively at fifth hour. The lethal times of methanol extract on 0.50% concentration were shorter than hexane extract e.g. 3.12 hours (LT50), 5.86 hours (LT90), and 7.00 hours (LT95) and for hexane extract on 0.75% concentration were 3.26 hours (LT50), 6.21 hours (LT90), and 7.45 hours (LT95). Water extract of 5% concentration was effective for traditional farmer in rural area due to easy and cheap method. The lethal concentrations of water extract on fifth hour were 2.02% (LC50), 4.00% (LC90), and 4.85% (LC95) and the lethal time on 5% concentration were 2.54 hours (LT50), 4.13 hours (LT90), and 4.75 hours (LT95). Key Words: Annona squamosa, Boophilus microplus, Water Extract, Methanol, Hexane

150 - 159

Authors: R Damayanti, A Wiyono

Malignant catarrhal fever in Indonesia is caused by Ovine herpes virus 2 and considered as a disease with high mortality rate causing degeneratif and lymphoproliferative disease in cattle, buffalo and other ruminants. A total number of fifteen Bali cattle were naturally infected by Malignant Catarrhal Fever (MCF). Those cattle were meant to be experimental animals of research on infectious bovine rhinotracheitis (IBR), Septicaemia epizootica (SE), and bovine brucellosis. The clinical signs of those animals were sudden high fever, depression, anorexia, corneal opacity, mucopurulent oculo-nasal discharges and diarrhoea. Six of them were dead and the remaining cattle were slaughtered at extremis. On the basis of clinical, gross-pathological and histopathological findings, all cases were shown to be consistent and pathognomonic of MCF cases. These cases were regarded as an outbreak of MCF affecting Bali cattle which occurred during wet season and while in other paddock in that area there were a number of lambing sheep. This result confirms that Bali cattle is a very susceptible animal of MCF and the cases were very likely due to the spread of MCF virus from lambing sheep. Key Words: Malignant Catarrhal Fever, Bali Cattle, Natural Infection, Pathology