Indonesian Journal of Animal and Veterinary Sciences

8 - 16

Authors: D Yulistiani, J.R Gallagher, R.J. Van Barneveld

Rice straw as one of agricultural by-products has low quality due to low content of essensial nutrients like protein, energy, minerals and vitamin as well as poor palatability and digestibility. Therefore, the quality of rice straw needs to be improved in order to increase its utilization by gastrointestinal tract of ruminants. The purpose of this study is to compare untreated and urea treated rice straw as basal diets for sheep. Twelve mature Merino wethers (average body weight 53.62 + 3.44 kg) were separated into 4 groups based on their live weight with each groups assigned three diets, that are: diet 1 untreated rice straw with high forage legume content, diet 2 urea ensiled rice straw and diet 3 rice straw sprayed with urea solution at feeding time. Diets were allocated based on a randomized complete block design. Urea ensiled rice straw was prepared by spraying chopped straw with urea solution to yield straw containing 4% urea and 40% moisture, then kept in air tight polythylene bags for 6 weeks. The untreated, ensiled and urea supplemented rice straw were mixed with other feed ingredients to provide isoenergetic and isonitrogenous diets. Diets were formulated to meet maintenance requirement according to NRC. Sheep were adapted to experimental diets for 15 days, and after adaptation period, a metabolism trial was conducted. Results reveal that dry matter intake permetabolic body weight (DMI/W0.75), DE (digestible energi) intake and apparent digestibility of NDF (neutral detergent fibre) were not significantly different between diet 1 and diet 2. Apparent digestibility of DM (dry matter), OM (organic matter), and ADF (acid detergent fibre), as well as N retention were not significantly different between three diets. Positive result in N retention was only observed in diet 2, while others were negative. It may be concluded from this study that untreated rice straw basal diet supplemented with forage legume offer an alternative method other than urea ensiled for improving the nutritional value of rice straw as a ruminant feed on small farmer. Key words: Rice straw, urea treated, digestibility, and sheep

26 - 39

Authors: I-W Mathius, D Sastradipradja, T Sutardi, A Natasasmita, L.A Sofyan, D.T.H Sihombing

Thirty-six Javanese thin-tail ewes in the end of late pregnancy phase were set out to study the energy and crude protein requirements during the first eight-week of lactation phase. The ewes were penned individually in doors and randomly assigned to a 3 x 3 factorial arrangement, consisting of three levels of energy (low, medium and high) and three levels of crude protein (low, medium and high) diets with four ewes per treatment. The diets were pelleted and offered four times daily in approximately equal amount. Feed intake, nutrient digestibility, body weight and milk production were recorded. Results showed that, total lamb birth weights was not affected, but protein content on the ration treatments significantly altered (P<0.05) milk yield and composition. Dry matter intake due to feeding treatments was not significantly affected (P>0.05), while crude protein content on the ration highly significantly affected (P<0.01). Based on data recorded, the energy and protein requirements for ewes during lactation phase are highly significantly depended on ewes’ live weight, milk production and the ratio of energy metabolism and crude protein of the ration. It was concluded that in order to fulfil the crude protein and energy needs of the ewes during lactation phase, the ration given should contain crude protein and energy as much as 16% (based on dry matter) and 13.4 MJ/kg dry matter respectively. Key words: Energy-protein requirement, lactation phase, local ewes

46 - 54

Authors: T Purwadaria, T Haryati, E Frederick, B Tangendjaja

Two successive experiments were conducted to determine the optimal substrate concentration of coconut meal (CM) and incubation time for production of β-mannanase from Eupenicillium javanicum. Both experiments were designed based on factorial. In the first experiment, the main factor was substrate concentration of 1, 2, and 3%, while the sub-factor was incubation time of 1, 2, 3, 4, 5, 6, and 7 days. The two factors were interacted highly significantly (P<0.01). Since the highest β- mannanase activity, protein concentration and saccharification activity towards coconut meal were obtained from 3% CM after five day incubation time (P<0.05), the second experiment was designed for higher substrate concentration. The main factor was also substrate concentration of 3, 4 and 5%, while the sub factor was incubation time of 5 and 6 days. The two factors were also interacted highly significantly (P<0.01) for mannanase activity and protein concentration, while specific activity was not significantly different (P>0.05). The best activity was obtained at 4% of coconut meal for five day incubation time, which was not significantly different with that of 3% at the same incubation time. Therefore, it was concluded from both experiments that the best enzyme production was obtained from 3% of coconut meal at incubation time of 5 days. Then, further experiments show that the enzyme had optimum pH at the range of 5.4-5.8, the same pH range in duodenum, while at pH 4.5 the activity was relatively low. Although, at pH 4.5 the enzyme activity was reduced, the enzyme was still active for four hours. At pH 5.8 and 6.5 the enzyme was quite stable. The optimum temperature of the enzyme was at 500C, higher than the body temperature of most poultry (400C). The reduction of enzyme activity at 400C could be overcome by increasing the enzyme concentration. The enzyme was stable after 4 hour incubation at 28 (room temperature) and 400C, however, the enzyme activity was considerably reduced at temperature of 900C after 60 second incubation. In the poultry digestion system the activity is not affected by temperature, but in the pelleting process where the steam temperature approximately 900C has to be limited for not more than 30 seconds. Key words: Coconut meal, β-mannanase, Eupenicillium javanicum, pH and temperature

64 - 70

Authors: Amlius thalib

Effect of microbial growth factors (FPM) combined with and without microbe preparate (SM) on growth of Etawah-crossgoat has been conducted for 14 weeks, including 2 weeks of adaptation period. Animals used were 24 male goats of Etawahcross (PE) with a mean liveweight of 17.73 ± 1.80 kg. The animals were randomly distributed into 3 treatment groups. Eachgroup consisted of 8 animals. All animals were fed elephant grass (ad lib.) + concentrate containing 16% crude protein (1.0% oflive weight) as basal diet. The treatment groups were : I. Control (K); II. K + FPM; III. K + SM + FPM. Measurements recordedwere: feed consumption, average daily gain (ADG), dry matter digestibility (in vitro and in vivo DMDs), as well as rumenecosystem. All animals were placed in metabolism cages for 2 weeks for determination of in vivo DMD. The results showed thatFPM combined with and without SM improved the performance of both rumen ecosystem and host animals. Compared tocontrol, combination of FPM with SM increased the following parameters significantly (P<0.05): ADG (55 vs. 36 g); DMI (645vs. 609 g head-1 day-1); in vivo DMD (74 vs. 69%); FCR (12 vs. 17); in vitro DMD (49 vs. 46%); colony number of bacteria percell number of protozoa (3.09 x 104 vs. 1.12 x 104); VFA content (3.53 vs. 2.82 mg ml-1); NH3-N content (68 vs. 56 mg l-1); pH(6.78 vs. 6.65). Microbe preparate enhanced the effect of FPM on VFA content so that the combination of FPM and SM(treatment III) significantly increased the VFA content as compared to the control (P<0.05). Key words: Microbial growth factor, microbe preparate, etawah-cross goat