Indonesian Journal of Animal and Veterinary Sciences

213 - 219

Authors: A.P Sinurat, T Purwadaria, T Pasaribu, J Darma, I.A.K Bintang, M.H Togatorop

Fermentation processes increase the protein of palm oil sludge and produce some useful enzymes. However, drying process, especially with heating often affects the nutritive values of feed ingredients and the enzymes activity. Therefore, this experiment was designed to study the responses of native chickens when fed ration containing fresh or dried fermented palm oil sludge (FPOS). Experimental diets with different levels of fresh or dried FPOS (5, 10 and 15% equal to dried FPOS) were formulated with similar nutrient contents. A control diet with no FPOS was also included. Each treatment was replicated 5 times for 12 weeks. Performances (body weight, feed consumption, feed conversion and mortality) of chickens were recorded. Carcass yield, abdominal fat content, weight of liver and gizzard were measured at the end of feeding trial. Data were subjected to analysis of variance and different between means were tested by orthogonal procedures. Results during the starting period showed that, chickens fed FPOS diet gain more weight significantly than the control birds (298.1 vs. 264.7). The dry matter intake of the dry FPOS diet was significantly better than the fresh FPOS diet (2.88 vs. 3.32). The FPOS dietary levels did not affect body weight gain and dry matter intake of native chickens, significantly. However, increasing the FPOS levels worsen the dry matter conversion (DCR) significantly. Data during 12 weeks trial showed mat the body weight gain was not significantly affected by treatments. The dry matter intake of the FPOS diets were significantly higher than the control diet (3469 vs. 3065 g/bird), hence the DCR of the control diet was significantly better than the FPOS diets (3.28 vs. 3.62). Feeding dry FPOS resulted in a better DCR as compared to fresh FPOS (3.48 vs. 3.76), but not affected the dry matter intake. The FPOS dietary levels did not affect body weight gain and dry matter intake of native chickens, significantly. However, inclusion of 5% FPOS in me diet gave better DCR significantly, as compared with 15% FPOS (3.51 vs. 3.83). Feeding dry or fresh FPOS (5 - 15%) did not significantly affect the mortality, carcass yield, abdominal fat levels, weight of liver and weight of gizzard of native chickens. It is concluded that dry FPOS was better than the fresh FPOS and could be included in me native chickens diet up to 10%. Key words: Palm oil sludge, fermentation, native chickens

227 - 232

Authors: E Handharyani, K Ochiai, W Winarsih, E Harlina, Salfina Nurdin Estuninngsih

The function of Ito cells is expanding from a fat-storing site to a center of extracellular matrix metabolism and mediator production in the liver. Immunohistochemical reactivities of Ito cells were examined in eight livers of broiler chickens affected with spontaneous cholangiohepatitis and six chicken livers with malformation of extrahepatic biliary tracts. The livers in both groups revealed severe diffuse fibrosis. Ito cells expressing HHF35 muscle actin and desmin actively proliferated in the fibrotic foci of the all livers. The immunoreactivities of Ito cells to antibodies were enhanced compared with those in normal livers. There were no immunohistochemical differences between the Ito cells of two groups. From these findings, it was suggested that Ito cells actively proliferate and show enhanced immunoreactivities in the livers affected with cholangiohepatitis andmalformation of extrahepatic biliary tracts. Key words : Cholangiohepatitis, immunohistochemistry, ito cell, broiler chicken

239 - 246

Authors: Rini Damayanti, Darminto

A study was carried out to detect the antigen of infectious bronchitis vius (IBV) in broiler chicken by means of immunohistochemical technique. A total of 150 - fourteen days old broiler chicken were divided into three groups i.e. 50 chicken were infected with an IB isolate of I-269, 50 chicken were injected with H-120 life vaccine, and 50 chicken served as un-treated control. Clinical signs and gross pathological changes were observed. Each of five chicken of each group were necropsied at 1, 2, 3, 4, 7, 10, 14, 21, 28, and 35 day(s) post infection/vaccination. The antigen could be detected at one day through 35 days post vaccination/infection. In the vaccinated group, histopathological lesions and the detected antigen were minimal. In contrast, the infected chicken showed varied histolopathological lesion in accordance with the numerous antigens. The antigen were observed in the lymphocytes/macrophages in the trachea, lungs and kidney, and in the epithelium of trachea, alveoli, broncheolus and tubular sitoplasm of the kidney of both vaccinated and infected groups. In the infected group, antigen was also detected in the lymphocytes and macrophages of the affected organs. Key words: Infectious bronchitis, broiler chicken, I-269 IB isolate, immunohistochemistry

254 - 258

Authors: G Joseph

An experiment was conducted to study the addition of NaHCO3 and Na2CO3 on acid-base status in swamp buffaloes, Three fistulated buffaloes were each introduced to dietary treatment control diett (50% rice straw + 50% concentrate), control + \0% NaHCO3 and diet control + 10% Na2CO3 in two times Latin Square Design. The diets contained 9,7% crude protein and 53% TDN to achieve maintenance requirements of the animals. Parameters measured include (l) Fed consumption, water consumption and urine volume. (2) pH in rumen fluid, saliva, bLood and urine, (3) natrium mineral content in rumen fluid, saliva, blood and urine. The results of the experiment showed higher pH in the rumen fluid, saliva, blood and urine of buffaloes due to supplementation of NaHCO3 and Na2CO3, Water consumption and urine volume was significanly increased as the effect of Na supplement. The acid-base status of buffaloes was apparently normal in all animals. Key words: Acid-base status, rice straw, concentrate, buffer, swamp buffaloes

265 - 268

Authors: S Widjajanti, S.E Estuningsih, Suharyanta

Observation on the antibody fluctuations of infected cattle with metacercariae of Fasciola gigantica and the effect of triclabendazole treatment were made by means of ELISA technique. Seven cattle were infected with 700 metacercariae and one cattle remained uninfected, as negative control animal. Treatment with triclabendazole was given to 6 cattle, when the mean antibody levels of infected cattle reached the peak, and the other one remained untreated, as positive control animal. One week after treatment the mean antibody levels started to drop and then decreased gradually. After eight weeks of treatment, the mean antibody levels of the treated cattle reached the lowest level or the same value as before infection, thereafter, 6 cattle were reinfected with different dosages of metacercariae of F. gigantica. Two cattle were infected with 400 metacercariae, the other two were infected with 600 metacercariae and the rest of them were infected with 800 metacercariae. The results showed that the immunological responses of re-infected cattle are quicker (5 weeks after infection) and the peak of the antibody levels are higher (ELISA OD = 1.7) than after the first infection (11 weeks after infection and ELISA OD = 1.2). However, after re-infection, there were no significant different on the antibody fluctuations and antibody levels among the infected group, although those cattle received different dosages. Key words: Antibody, cattle, Fasciola gigantica, ELI

274 - 278

Authors: E Handharyani, K. Ochiai, W. Winarsih, E. Harlina, S. Estuninngsih

The function of Ito cells is expanding from a fat-storing site to a center of extracellular matrix metabolism and mediator production in the liver. Immunohistochemical reactivities of Ito cells were examined in eight livers of broiler chickens affected with spontaneous cholangiohepatitis and six chicken livers with malformation of extrahepatic biliary tracts. The livers in both groups revealed severe diffuse fibrosis. Ito cells expressing HHF35 muscle actin and desmin actively proliferated in the fibrotic foci of the all livers. The immunoreactivities of Ito cells to antibodies were enhanced compared with those in normal livers. There were no immunohistochemical differences between the Ito cells of two groups. From these findings, it was suggested that Ito cells actively proliferate and show enhanced immunoreactivities in the livers affected with cholangiohepatitis and malformation of extrahepatic biliary tracts. Key words: Cholangiohepatitis, immunohistochemistry, ito cell. broiler chicken