Revista MVZ Cordoba

e1875

Authors: Leticia Cañas L; Sandra Hernández-Dávila; Juan Carlos Vázquez-Chagoyan; Simón Martínez-Castañeda; Raúl Fajardo M; Benjamín Valladares-Carranza; César Ortega S

Objective. To perform the isolation of spring viremia of carp virus (SVCV) in common carp (Cyprinus carpio) and evaluate its growth in different cell types and viral survival at different temperatures. Materials and methods. Ten carps of between 400-500 grams of a lagoon in central Mexico were processed for diagnosis of SVCV by isolation in cell culture and by RT-PCR. The virus obtained was inoculated into EPC, BF-2, CHSE-214 and RTG-2 cells to determine differences in virus growth; the survival of virus stored at room temperature (TA 20-25°C), refrigeration (REF 4°C) and freezing (CONG -80°C) up to eleven months was also evaluated. Internal organ samples were processed for histological analysis. Results. The fish analyzed did not show external signs suggestive of disease but internally and histopathologically lesions suggestive of systemic infection were observed. SVCV was isolated in EPC and BF-2 cells and confirmed by semi-nested RT-PCR. SVCV only induced CPE in EPC and BF-2 cells and was negative in RTG-2 and CHSE-214. The virus conserved at TA lost viability after four months post-infection (mpi), being total at six mpi; while REF and CONG were stable during the eleven months. Conclusions. Subclinical SVCV infection was confirmed in carp that presented histological lesions associated with this infection; SVCV only caused CPE in EPC and BF-2 cells; and the virus kept in refrigeration and at -80°C retained its viability up to eleven months; while TA was lost in six months.

e1982

Authors: Liliana Rojas-Risco; Paola Montalván-Damián; Juan R. Paredes-Valderrama

Objective. To determine the haematological parameters according to the reproductive status of Peruvian Paso horses in the Province of Lambayeque. Materials and methods. The research was conducted on 60 clinically healthy adult horses (> 4 years), without previous drug treatment. Four groups of 15 horses were formed: gelding, entire male, empty mare and pregnant mare. First, a general clinical examination was performed to confirm their good health. Blood was drawn from the jugular vein of all the horses. The samples were processed by haematological analysis and the parameters of the red series (haemoglobin, haematocrit, red blood cells, mean corpuscular volume [MCV], mean corpuscular haemoglobin [MCH] and the mean corpuscular haemoglobin concentration [MCHC] were determined. Likewise, the white series (leukocytes, neutrophils segmented and stacked, basophils, monocytes, lymphocytes, eosinophils) and the thrombocytic series were processed and the unit range determined. Manual methods, mathematical formulas and the Drabkin method were used in the clinical pathology laboratory. The Kruskal Wallis Statistical test was applied and consequently the mean, standard deviation and ranges of variables were obtained. Results. No significant statistical differences were found in the white, red and thrombocytic series according to sex or reproductive status, except the number of basophils was higher in the entire males. Conclusions.The haematological parameters according to the reproductive status of the Peruvian Step horse are within the normal ranges (as per the literature pertaining to this area). However, the number of lymphocytes was much lower.

e1720

Authors: Raúl Molina-Benavides; Clarita Bustamante Z; Adriana Martínez A; José Uribe C; Johan Redondo O

Objective. Use Geographic Information Systems (GIS) as a complementary tool to characterize cattle farming in the Orinoquia region. Materials and methods. Through the use of space technologys, information concerning the livestock orientation, physiography, vegetation cover and land registry of the study zone was collected for further analysis over Microsoft ACCESS software. Results. In a high percentage of the cattle ranches located in the four departments (Casanare: 72.7%, Meta: 49.5%, Arauca: 42% and Vichada: 32%) the cover of pastures, grasslands and secondary vegetation predominates, confirming the expansion in the agricultural border that has had the cattle activity in the country. Conclusions. The use of complementary tools such as GIS allows for better planning and efficient distribution of resources to improve the functioning of production systems, for example, in zones where the predominant coverage matrix is grasslands, strategies in pro of sustainability can focus on the implementation of silvopastoral systems, contrary to what would happen in areas where the matrix has a high percentage of natural forests

e1854

Authors: Julio Cesar Vargas-Abella; Luis Edgar Tarazona-Manrique; Roy José Andrade-Becerra

Objective. Microbiologically characterize dry and frozen pollen produced in the municipality of Viracachá-Boyacá. Materials and methods. Through a quantitative descriptive cross-sectional study, samples from 5 apiaries were taken, each with 10 hives, separating the pollen dry and frozen, determining for each sample: mesophilic aerobes, total coliforms, fecal coliforms, Staphylococcus aureus, Clostridium sulfite reducer, and fungus. The data obtained were analyzed according to international regulations and compared with research results in other countries. Results. Total and fecal coliforms were found in three of the five apiaries evaluated and only in dried pollen samples. Also, in two apiaries when dry pollen was analyzed, Staphylococcus aureus was found. The microbiological results of most samples are within the ranges of some international regulations; however, the best results in terms of microbiological quality were determined for frozen pollen. Conclusions. The pollen freezing process offers advantages related to maintaining microbiological quality compared to the drying process. It is necessary to evaluate the microbiological quality of both products throughout the storage time.

e1816

Authors: Nathalia Correa-Valencia; Roberto Damián Moyano; María Isabel Romano; Jorge A Fernández-Silva

Objective. To determine Mycobacteriumavium subsp. paratuberculosis (MAP) molecular diversity in environmental samples from Colombian dairy herds. Materials and methods. Environmental samples from 25 IS900-qPCR MAP-positive dairy herds were cultured by duplicate in Herrold ́s egg yolk medium with mycobactin J to obtain isolates. Suspicious colonies were confirmed by MAP-IS900-qPCR. Positive DNA was sub-typed using mycobacterial interspersed repetitive units-variable number of tandem repeat (MIRU-VNTR) and multilocus short sequence repeats (MLSSR) techniques to analyze the genetic differences between the isolates. Results. Sub-typing revealed two different genotypes by MIRU-VNTR (INMV 2 and INMV 36). MLSSR technique was carried out to increase the discriminatory power from what was obtained by MIRU-VNTR, but no differences were observed among the recovered isolates. Conclusions. The present study represents an important approach to the knowledge on MAP epidemiological status in the study population.

e1876

Authors: David Contreras-Marquez; Juan Quintero-Pardo; Edwin Correa-Rojas; Emiro Canchila-Asencio; Heidy Gutiérrez-Zocadagui

Objective. To quantify the concentration and consumption of the protein fragments (NPN, TP, RDTP, SDTP, NDIP, and ADIP) present in the diet supplied to cattle, and its effect on the digestibility of crude protein. Materials and methods. Diets offered to Chino Santandereano cattle in stables, receiving different levels of supplementation, were analyzed. Using four animals in the 4x4 Latin square design, the treatments were UNS, not supplemented; LOW, supplemented with an amount relative to 0.5% of body weight; MEDIUM, supplemented with an amount relative to 1.0% of body weight; HIGH, supplemented with an amount relative to 1.5% of body weight. Consumption was determined daily, and digestibility through total stool collection in the last two days of each period. Results. A higher concentration of crude protein was found in the supplement than in grass (p<0.001), the forage exhibiting a higher concentration of CPNPN (p<0.001). The supplement also presented a higher concentration of TP (p<0.001) and RDTP (p=0.027). Supplemented animals presented higher consumption of CP, CPNPN (p=0.037), TP, NDIP, RDTP, ADIP, and SDTP (p<0.05), however, when the concentration representing the consumption of ADIP in the consumption of CP was determined, no difference was observed between supplemented and UNS (p=0.078). Higher digestibility of CP was found in supplemented animals than in UNS (p<0.001), and an upward linear effect was observed between supplemented treatments as the level of supplementation increased. Conclusions. Supplementation improves the digestibility of crude protein by providing a greater amount of highly digestible nitrogenous fragments.

e1944

Authors: Johan Romero-Herrera; Lina Henao-Montoya; Henrique Guimarães-Riva; Jeiczon Jaimes Dueñez

Objective. The aim of this work was to characterize and describe the frequency of the gastrointestinal parasites in synanthropic rodents captured at the Barranquilla Zoo.Materials and methods. The rodents were captured using Tomahawk traps with the help of non-toxic bait and subsequently sacrificed by isoflurane inhalation. The diagnosis and identification of parasites in the intestinal contents was performed using direct examination in NaCl (0.9%), modified Sheather’s flotation, and modified Ritchie’s sedimentation techniques. Results. A total of 34 rodents (Rattus rattus) were captured, with an infection frequency of 82.4% (95% CI = 68.8–95.8%, 28/34). The most prevalent parasites were nematodes (63.2%, 95% CI = 49.2–77.2%, 31/49), followed by protozoans (20.4%, 95% CI = 8.7–32.1%, 10/49) and cestodes (16.3%, 95% CI = 5.6–27%, 8/49), with significant differences between the three groups (χ² = 20.7, p< 0.01). Among the nematodes, Strongylidae was the most prevalent family (24/31), followed by Ascarididae (4/31), Ancylostomatidae (1/31), Oxyuridae (1/31), and Trichostrongylidae (1/31); for the protozoans, the most prevalent families were Eimeriidae (6/10) and Entamoebidae (4/10), and for the cestodes, the only family found was the Hymenolepididae (8/8) family. Conclusions. The R. rattus species was the only species of synanthropic rodents caught in the Barranquilla Zoo; because it was infected with various species of gastrointestinal parasites, it should be considered a risk factor for the transmission of these parasites to humans and other animals in the zoo.

e2114

Authors: Marco González T; Luis Salgado-Arroyo

Objective. Analyze the scientific production in Veterinary Medicine and Animal Husbandry in Colombia in the period 2010-2019. Materials and methods. A retrospective cross-sectional descriptive study was carried out, through the bibliometric analysis of articles published in nine journals in the area of Veterinary Medicine and Animal Husbandry in Colombia. The information was obtained by searching the internet for each journal’s website. The articles included were original articles, literature reviews, brief communications, clinical cases and editorials. Results. The total number of articles was 3.000; 2.250 (75%) published in Spanish, 673 (22.4%) in English and 77 (2.6%) in Portuguese. 2.282 (76.1%) were original, 224 (7.5%) literature reviews, 165 (5.5%) clinical cases, 105 (3.5%) brief communications, 204 (6.8%) editorials and 21 (0.6%) others. There were 10,296 authors; 7.109 (69%), nationals and 3.187 (31%) foreigners. Of the total Authors, 6.659 (64.7%) were men; 3.199 (31%) women and 438 (4.3%) without identification, with an average of 3.38 authors/article. Public entities contributed 77.9% of scientific production and private entities 22.1%. The areas with the highest number of publications were zootechnics, animal health, agriculture and wildlife. The top 5 of affiliations of the authors corresponded to public universities. Conclusions. The journal that publish in English achieve a better international positioning and in them the largest number of foreign authors publishes. The most published articles were the original ones. The top 10 of the most cited articles yielded a total of 810 citations that contribute to the internationalization of Colombia and Colombian journals

e2052

Authors: Irma Yupari-Azabache; Jorge Díaz-Ortega; Ángela Rodríguez-Diaz; Ana Peralta-Iparraguirre

Objective: This research aims to analyze the relation between biological, social and cultural factors regarding the attitudes and preventive measures against COVID-19 among citizens of Trujillo- Peru. Material and Methods: This is a descriptive research, with a quantitative approach and a correlational, prospective and design. A previously validated questionnaire was used to collect certain biological, social and cultural data, as well as attitudes and preventive measures against COVID-19 among 185 citizens of Trujillo. Due to the quarantine and the Sanitary National Emergency, the collection of data was done by virtual means. Results: Citizens between 18 and 29 years old have inappropriate attitudes (12.4%) and inappropriate preventive measures (8.6%). Also, males have inappropriate attitudes and inappropriate preventive measures. Moreover, single citizens or unmarried couples without children have inappropriate attitudes (14.1%) and inappropriate practices (13%). Conclusions: Among biological factors, age and gender are related to attitudes and preventive measures regarding the pandemic. Among social factors, marital status and parenthood are related to attitudes and preventive measures regarding the pandemic. And among the cultural factor, knowledge of this disease and the measures to be taken are related to the preventive measures against COVID-19.

e2004

Authors: Patrícia Glombowsky; Gabriela Campigotto; Maiara Sulzbach Marchiori; Jorge A. Favaretto; Gabriela Miotto Galli; Aleksandro Schafer da Silva

Dogs with Giardia infections have diarrhea that persists for days, even after treatment. We managed canine giardiasis using two distinct approaches: treatment with the antiprotozoan secnidazole alone; and secnidazole plus a homeopathic product that is indicated for control of diarrhea in dogs. Treatment I: Ten beagle dogs showed clinical signs of severe diarrhea. Their feces revealed large numbers of cysts consistent with Giardia sp. Therefore, we treated the dogs with secnidazole (10 mg/kg, single dose) given orally. Five days after treatment, all dogs were negative for Giardia spp. Treatment II: Three months later, the same ten Beagles again presented with diarrhea. Feces were collected, revealing Giardia sp. in all dogs. This time, we treated them with an antiprotozoan (secnidazole, 10 mg/kg dose) combined with a commercial homeopathic drug for diarrhea control (3 g dose/animal at 12 h interval). We found that three to five homeopathic doses were needed to achieve normal fecal scores in all dogs. After 5 days of treatment, stool samples had normal consistency and were negative for Giardia. This is the first record of using secnidazole to control canine giardiasis associated with homeopathic drugs to prevent diarrhea.