Revista MVZ Cordoba

e1611

Authors: Matiluz Doria R; Misael Oviedo P; Teresa Oviedo S; Mario Canabal V; Juan Pérez G; Juan Fernández N

Objective. Determine the seroprevalence and epidemiological factors associated with Mycobacterium avium subsp. paratuberculosis (MAP) in two Creole cattle breeds of the Turipaná research center - AGROSAVIA. Materials and methods. A cross-sectional epidemiological study was conducted,a total of 848 Creole bovine animals were sampled, 403 Romosinuano and 445 Costeño Con Cuernos (CCC); for the serological diagnosis of antibodies, the Elisa Indirect test was used with the commercial kit Parachek®2 by Prionics. The variables sex, age, breed and herd type were the epidemiological factors evaluated and correlated with the presence of antibodies against MAP; the statistical association was established using the Odds Ratio and a multivariate logistic regression model, employing a significance level with p<0.05. Results. The general seroprevalence of the Creole cattle to MAP was 2.35% (95% CI, 1.34-3.38); however, in the Romosinuano it was 0.74% and in the CCC it was 3.82%, being this difference statistically significant (p=0.003). Furthermore, employing a univariate way analysis, females and animals older than one year of age were more affected. The multivariate analysis identified the breed and sex variables as epidemiological factors. Conclusions. In the Creole breeds of the AGROSAVIA-Turipaná research center, MAP seroprevalence was low; however, in the Costeño Con Cuernos breed, the risk of contracting the disease is six times higher than in the Romosinuano breed. Moreover, it was shown that females have a higher risk of acquiring the disease.

e1708

Authors: Catalina Medrano-Galarza; Aldemar Zuñiga-López; Fredy E. García-Castro

Objectives. The aim of this study was to determine an overall on-farm animal welfare score (WS) for dairy farms using a protocol involving animal- and management/resource-based variables; and to identify the variables that contributed the most to the variance for the WS. Materials and methods. Twenty-five pasture-based dairy farms in Savanna of Bogota - Colombian high tropic, were visited twice. During each visit, a total of 15 animal- and 13 management/resource-based variables were evaluated. For each variable, a herd-level negative cut-off point was established to determine compliance. Based on compliance, a WS was determined (from 0 to 100) and farms were classified as ‘excellent’, ‘enhanced’, ‘acceptable’, or ‘non-acceptable’. A linear regression model was used to evaluate the association between variables at the herd-level with the WS. Results. Overall, median WS was 82 (min = 67; max = 97). Two farms were classified as ‘excellent’ (WS = 94.5), 20 as ‘enhanced’ (WS = 82.5), and 3 as ‘acceptable’ (WS = 68). Among all variables, the noncompliance regarding subclinical mastitis, hind-legs and udder dirtiness score, ventral line wounds, Haematobia irritansfly counts, and condition/cleanliness of pre-milking holding area was significantly associated with a 5.6, 3.6, 6.7, 5.1, 6.5, and 6.1 points reduction in the WS, respectively (R2 = 0.84). Conclusions. Welfare level found across farms was good and encouraging. Nonetheless, six variables were identified as the main contributors to an unfavorable result. Strategies for improvement could focus on these variables to be able to reach a welfare level of ‘excellence’.

e1863

Authors: Bernardo Reyes-Bossa; Hernán Medina-Ríos; José A. Cardona-Álvarez

Objective. To determine the ultrasonographic morphometric parameters of the palmar metacarpal tendons and ligaments of clinically healthy Colombian creole horses. Materials and methods. Thirty clinically healthy Colombian creole horses of 6.4 ± 2.5 years were evaluated in different areas of the department of Córdoba (Colombia). The variables cross-sectional area (cm2), medial lateral width (ALM) (cm) and dorsal palmar thickness (EDP) (cm) in the structures of the metacarpal region in the two anterior members were measured by means of ultrasound. Results. Ultrasonography revealed that the transverse area of the suspensory ligament was the structure with the largest area in the proximal regions (1A, 1B and 2A), for both the left and right limbs. On the other hand, there was no significant difference (p>0.05) between left and right limb for measurements of structures in any area, and there was no significant difference between age groups and body mass index (BMI) of horses. Conclusions. Reference values for tendons and ligaments in the palmar metacarpal region were obtained in equines providing a reference guide when metacarpal pathological conditions in equines are suspected.

e1809

Authors: María Largo; Jhon Tuberquia ; Mauricio Sánchez ; Mariana Machado ; Santiago Rodríguez , Santiago Monsalve B; Nathalia Correa-Valencia; Jorge A. Fernández-Silva

Objective. To determine the presence of anti-Mycobacterium avium subsp. paratuberculosis (MAP) antibodies in white-tailed deer (Odocoileus virginianus) and red brocket deer (Mazama rufina), captured in the Orinoquía and Caribbean regions in Colombia. Materials and methods. A total of 44 blood serum samples from deer of species M. rufina and O. virginianus werecollected under field conditions between 2014 and 2016. An ELISA commercial kit was used to detect anti-MAP antibodies. An animal was considered ELISA-positive at a sample-to-positive ratio (S/P%) of ≥ 0.4, as recommended by the manufacturer. Results. The 50% (22/44) of the animals were positive, corresponding to 10 females and 12 males. An 81.8% (18/22) and 77.3% (17/22) of these seropositive animals were captured in the Orinoquía region and were reported as adults, respectively. Conclusions.It is not known how or when MAP was introduced in the Colombian deer population in the study regions. The most plausible hypothesis to explain the presence of antibodies against MAP in these wild populations is transmission by contact with infected bovine cattle since, in both regions, these species share pastures. This is the first study to explore MAP infection in wild animals in Colombia. These findings support the need for further studies using different direct diagnostic techniques and research approaches that allow the definition of links in the infection dynamics between wild and domestic mammals in Colombia.

e1845

Authors: Soledad Ruiz P; Cecilia Baumberger; Pedro Jimenez-Bluhm;Víctor Marambio; Carla Salazar; Christopher Hamilton-West

Research and interest in avian influenza virus have increased considerably in recent decades in response to highly pathogenic avian influenza outbreaks in poultry and its zoonotic potential. Wild waterfowl are the main reservoir of the virus, therefore studying the dynamics of influenza A virus (IAV) infection in these populations is essential in order to understand its potential persistence in the environment and transmission to poultry and humans. It has been identified that environmental factors (such as temperature, rainfall, vegetation and landscape characteristics, among others) can play an important role in the maintenance and dissemination of the virus in the areas of concentration of wild birds. However, studies that include ecological aspects of the virus and explore the interaction between the prevalence of IAV in wild birds and environmental factors remain scarce. This review summarizes research efforts that have been made to identify the environmental factors involved in the persistence and transmission of IAV in areas of wild bird concentration and how these factors may influence the prevalence of the virus in these populations, generating differences in the presentation of the infection among different geographical areas.

3

Authors: Salim Máttar1; Marco González T

For some years now, the editorial space of the Journal MVZ Córdoba has been oriented towards addressing human and animal public health issues under the “One Health” concept. We have valued and analyzed scientific articles, issued opinions, sparked debates, proposed lines of research and we have also predicted and warned of the arrival of new diseases both in the country and on the continent.

7

Authors: Andrés Góngora-Gómez; Lizeth Villanueva-Fonseca; Pedro Sandoval-Rivera; Juan Hernández-Sepúlveda; Ana L. Domínguez-Orozco; Brenda Villanueva-Fonseca; Manuel García-Ulloa

Objective. To detect the presence of presumptive hypnospores of the protozoan Perkinsus sp. in a wild population of the Mexican chocolata clam Megapitaria squalida in the southeastern Gulf of California, using Ray ́s fluid thioglycollate medium (RFTM). Material and Methods. Thirty specimens with size between 56.17 and 69.04 mm were captured each month, during an annual cycle. Infection prevalence and intensity and water parameters were recorded monthly from September 2012 to September 2013. Results. Perkinsus sp. was detected in tissue samples from the Mexican chocolate clam using the RFTM test by the presence of dark round corpuscles that represent parasite ́s hypnospores.Monthly samplings revealed a prevalence of 0-43.33% and an infection intensity ranging from 1 to 4 (no infection = 0 hypnospores/entire preparation, to moderate = 34 hypnospores/entire preparation). Conclusions.Perkinsus sp. is reported for the first time in a wild population of M. squalida in the southesternmost Gulf of California. The results indicate that this protozoan is dispersed intraspecifically and would now, potentially, parasiting a new host in the region.

10

Authors: Marco Torres-Castro; Viviana Febles-Solís; Silvia Hernández-Betancourt; Henry Noh-Pech; Erendira Estrella; Ronald Peláez-Sánchez; Alonso Panti-May; Belén Herrera-Flores; Bibiana Reyes-Hernández; Javier Sosa-Escalante

Objective. To report the infection with Leptospira in the kidneys of bats from Campeche and Yucatán, Mexico, through the amplification by PCR of two different 16S RNA ribosomal gene fragments. Materials and methods. Bat captures were carried out at one site in Campeche and two sites in Yucatán. Euthanasia was applied to the captured bats and a necropsy was performed to collect a renal tissue sample that was used in the total DNA extraction. Two different conventional PCR were performed for the amplification of the 16S RNA ribosomal gene fragments. Some sequences from positive products were obtained and analyzed with bioinformatics tools to identify the infectious species of Leptospira. Results. Sixty-nine bats belonging to four families and eight different species were captured. The family with the greatest diversity was Phyllostomidae, with five species. The most captured species was Artibeus jamaicensis (41, 59.4%). Both PCR showed a global infection frequency of 21.7%. The infected species were A. jamaicensis, Pteronotusparnellii and Chiroderma villosum. The bioinformatic analysis of the positive products yielded a 99.0% identity for Leptospira noguchii, Leptospira borgpetersenii, and Leptospira santarosai. Conclusions. Some bat species of Yucatán and Campeche, Mexico, are renal carriers of pathogenic Leptospira, therefore participating in the transmission cycle in the region. The frequency of infection found in the renal tissue of the captured bats is higher than the one obtained from other reservoirs captured in Yucatán and Campeche. New species of bats are reported as renal Leptospira carriers in Mexico.