INDO AMERICAN JOURNAL OF PHARMACEUTICAL SCIENCES

1468-1473

Authors: B. Shailaja, M. Afreen, T. Vinay Kumar, A. Ashok Kumar

Objective: To develop a simple and cheap UV spectrophotometric simultaneous equation method for the simultaneous quantitative estimation of Etodolac (400mg) and Paracetamol (500mg) in tablets and validate as per ICH guidelines. Method: The optimized method uses 100% Triethylammonium phosphate buffer (pH 10) as a diluent for the simultaneous quantitative estimation of Etodolac and Paracetamol in tablets by UV spectroscopy by simultaneous equation method. Results: The developed method exhibited linearity in the range of 5-15μg/ml for Etodolac and 6.25-18.75μg/ml for Paracetamol. The precision for Etodolac and Paracetamol is exemplified by relative standard deviation of 0.176% and 0.246 respectively. Percentage Mean recovery for EtodolObjective: To develop a simple and cheap UV spectrophotometric simultaneous equation method for the simultaneous quantitative estimation of Etodolac (400mg) and Paracetamol (500mg) in tablets and validate as per ICH guidelines. Method: The optimized method uses 100% Triethylammonium phosphate buffer (pH 10) as a diluent for the simultaneous quantitative estimation of Etodolac and Paracetamol in tablets by UV spectroscopy by simultaneous equation method. Results: The developed method exhibited linearity in the range of 5-15μg/ml for Etodolac and 6.25-18.75μg/ml for Paracetamol. The precision for Etodolac and Paracetamol is exemplified by relative standard deviation of 0.176% and 0.246 respectively. Percentage Mean recovery for Etodolac and Paracetamol were found to be in the range of 90‐110, during accuracy studies. Conclusion: A simple and a cheap UV spectrophotometric method was developed and validated for the simultaneous quantitative estimation of Etodolac and Paracetamol in tablets as per ICH guidelines and hence it can be used for the routine analysis in various pharmaceutical industries. Keywords: UV, Etodolac, Paracetamol, Assay, validation. ac and Paracetamol were found to be in the range of 90‐110, during accuracy studies. Conclusion: A simple and a cheap UV spectrophotometric method was developed and validated for the simultaneous quantitative estimation of Etodolac and Paracetamol in tablets as per ICH guidelines and hence it can be used for the routine analysis in various pharmaceutical industries. Keywords: UV, Etodolac, Paracetamol, Assay, validation.

1486-1490

Authors: Md. Sahab Uddin* , Md. Fakhrul Hasan, Abdullah Al Mamun, Md. Sarwar Hossain, Md. Tanjir Islam, Md. Asaduzzaman

Background: Complementary and alternative medicine based on plants is the world's oldest form of medicine and recent reports suggest that such therapies still enjoy vast popularity, especially in developing countries where most of the population does not have easy access to modern medicine. Aim: The objective of this study was to evaluate the antioxidant activity of chloroform fraction (CLF), carbon tetra chloride fraction (CTF) and n-Hexane fraction (NHF) of methanolic extracts of Caryota urens (CU) fruits. Method: For determination of antioxidant property of the CU fruits extracts, DPPH (2,2‐diphenyl‐2‐picrylhydrazyl) radical scavenging assay was performed. Results: Among three different fractions CLF showed the highest antioxidant activity (61.58 % scavenging) at 400 µg/ml concentration followed by CTF and NHF. The IC50 values for the DPPH radical scavenging test were in the order of CLF (93.45 ± 3.09 µg/ml) > CTF (473.01 ± 12.95) > NHF (613.13 ± 7.64). Conclusion: Our study suggested that CLF of CU fruits had strong antioxidant effect compared to CTF and NHF. Keywords: Complementary and alternative medicine, Caryota urens, Antioxidant activity, DPPH radical scavenging assay.

1506-1509

Authors: Navya Devireddy*, Divya Jonnala, Fareeda Rafath, Sushmitha Gutti, D.Eswar Tony, Rama Rao Nadendla

The Uniject injection system is an auto disable injection device that was designed to meet persistent logistics, safety and cost effectiveness. Several studies indicate that Uniject device is safer, poses less risk of needle stick, and is more efficient because it is already prefilled with the right amount of medicine or vaccine. Uniject injection delivery system was successfully used and administered to 75million doses of vaccines and other medicines worldwide. The present review highlights the structure, functions, applications and also advantages of the uniject device. Key words: Uniject, tetanus toxoid, Hepatitis B vaccine.

1515-1517

Authors: Hariprasath Kothandam(PhD), Deepthi Babu(Pharm D), Haripriya Babu(Pharm D) and Sheethal R Johnson(Pharm D),

Cancer is primarily caused by mutations in growth, growth-inhibiting factor genes and pathways that inhibit the normal sequence of events associated with apoptosis. Normally cell cycle is controlled by signal transduction. Generally growth factors stimulate cell division and growth-inhibiting factors inhibit cell division. Healthy cells divide only when growth factor and growth-inhibiting factor balance favors cell division. Cancer cells divide without constraint. This paper reviews about the genes evidencing the possibility of cancer causing mechanism. Key words: Mutation, Cell cycle, Signal transduction, Cell division and Genes.

1525-1530

Authors: M. Divya*, Dr. H. Padmalatha

A simple, rapid, precise, specific and accurate RP-HPLC method has been developed for the determination of Darifenacin, a drug is of Urinary antispasmodic in bulk and pharmaceutical dosage form. Chromatography was performed on a Hibar® (250 x 4 mm, 5μm) LiChrosphere® 100 RP-18 in an isocratic mode with mobile phase Acetonitrile: 10mM Tetra Butyl Ammonium Hydrogen Sulphate (70:30%v/v) was used. The flow rate was 1.0ml/ min and effluent was monitored at 285 nm. The retention time was 3.108 min & linearity range was found to be 5 - 300 g/ml and the %RSD of the method was found to be less than 2%. The proposed method was statistically validated. The excipients present in the formulation do not interfere with the assay procedure. Key Words: Darifenacin hydro bromide, Validation, RP-HPLC

1545-1549

Authors: Md. Asaduzzaman

Background: Medicinal plants are the mainstay of complementary and alternative medicine. The search for new natural remedies is still ongoing process against new and existence diseases. Aim: The objective of this study was to evaluate the phytochemical constituents and cytotoxic activity of methanolic extracts of Aegle marmelos (Linn.) (MEAM) leaves and its petroleum ether fraction (PEF), chloroform fraction (CLF), ethyl acetate fraction (EAF) & aqueous fraction (AQF). Method: A crude methanol extract and four fractions were prepared from the leaves of Aegle marmelos (AM). The preparations were assessed for phytochemical analysis by saponins, tannins, glycosides, steroids and alkaloids tests and cytotoxic activity by brine shrimp lethality assay. Results: Phytochemical screening of CME showed the presence of saponins, tannins, glycosides, steroids and alkaloids. Glycosides and steroids were found in the PEF. CLE showed the presence of only steroids and EAF showed the presence of only glycosides. AQF was highly enriched in saponins, tannins, glycosides and alkaloids. For cytotoxic activity among the extractives, the most potent activity was found in PEF and the ED50 value of PEF was 24.00 g/ml. The ED50 values of other extractives such as CME, CLF, EAF and AQF were 40.00, 150.00, 184.63 and 240.02 g/ml respectively. The ED50 of vincristine sulphate was found to be 1.04 g/ml. Conclusion: From the study it was found that AQF of AM leave enriched in phytoconstituents and PEF had significant cytotoxicity activity compared to other extracts. Keywords: Aegle marmelos, Phytochemical Screening, Cytotoxic Activity, AQF, PEF.