INDO AMERICAN JOURNAL OF PHARMACEUTICAL SCIENCES

1141-1146

Authors: Dr.Satish Kumar NS1,MD, Mrs.Pavana K2,Dr.Aswini Dutt R3, MD

Background:Type 2 diabetes and obesity have reached epidemic proportions all over the world. Women will account for the majority of diabetic cases by the year 2050. Effect of age on Cardio-metabolic risk factors in red meat consuming female type 2 diabetic patients needs to be studied. Objective:The aim of this study was to retrospectively assess the relation between red meat consumption and glycemic control, blood pressure and obesity in young and aged female patients of type 2 diabetes. Methods: This is a retrospective study done from the medical records of the patients of type 2 diabetes attending the YMC hospital, Mangalore. The study group consisted of female diabetic patients predominantly consuming red meat diet aged between 27 to 65 years. They were divided into two groups based on their age (viz. <40 years, >40 years of age). Their FBS, PPBS, Blood pressure and BMI were noted. These parameters between two age groups of red meat consumers were measured by unpaired t-test. Results: The data when analysed statistically revealed that female diabetic patients aged above 40 years was positively associated with poor glycemic control in terms of both FBS and PPBS(p < 0.0005).There was significantly higher BMI and BP in elderly female diabetic patients (p < 0.0005 & p< 0.005 respectively). Conclusions: We found that a consumption of red meat was associated with poor glycemic control, hypertension and obesity in patients of type 2 diabetes in elderly-aged women, which is likely due to their hormonal fluctuations. Keywords: Diabetes, Red Meat, Blood Pressure, Body mass Index, Female

1154-1166

Authors: M.Naga Ganesh* , Jamjala Naveen, T.Mangilal,M.Ravi Kumar

he present investigation is aimed to formulate floating gastroretentive tablets containing Quetiapine Fumerate using a sublimation material. The effect of the amount of HPMC K4M on swelling and eroding of the tablets was determined. The water-uptake and erosion behavior of the gastroretentive tablets were highly dependent on the amount of HPMC K4M. The water-uptake increased with increasing HPMC K4M concentration in the tablet matrix. The weight loss from tablets decreased with increasing amounts of HPMC K4M. Camphor was used as the sublimation material to prepare gastroretentive tablets that are low-density and easily floatable.Floating properties of tablets and tablet density were affected by the sublimation of camphor. Prepared floating gastroretentive tablets floated for over 24 hrs and had no floating lag time. However, as the amount of camphor in the tablet matrix increased, the crushing strength of the tablet decreased after sublimation. The release profiles of the drug from the gastroretentive tablets were not affected by tablet density or porosity. From the Formulation, Kinetic, FTIR and DSC Studies indicated that the drug was stable in the tablets. HPMC K4M can be used as a rate controlling polymer by appropriate selection in different ratios. The release of the drug from a matrix tablet was highly dependent on the polymer concentrations. Key words: Gastroretentive Quetiapine fumerate floating tablets, Effervescent method, Non Effervescent method,

1173-1177

Authors: Swetha1 , B. Mohammed Ishaq*1 , Hindustan Abdul Ahad1 , Vanitha Prakash2

Lenalidomide, a thalidomide analogue, is an immune-modulatory agent with antiangiogenic and antineoplastic properties. The chemical name is 3-(4-amino-1-oxo 1,3-dihydro-2H-isoindol-2-yl) piperidine- ,6-dione. Lenalidomide is indicated for the treatment of patients with transfusion-dependent anemia due to Low- or Intermediate-1-risk myelodysplastic syndromes associated with a deletion cytogenetic abnormality with or without additional cytogenetic abnormalities. Lenalidomide possesses antineoplastic, immuno- modulatory and antiangiogenic properties. The present work explains the development and validation of a simple and reliable liquid chromatographic method for the quantitative determination of Lenalidomide in bulk and in tablet formulation. Chromatography was carried out by reversed phase technique on a Waters X-terra RP 18 (250mm x 4.6mm x 5µ) with a mobile phase composed of Methanol and Acetonitrile in the ratio of 60:40 (v/v) pumped at a flow-rate of 1.0 ml/min. The detection was carried out at 210 nm at the ambient column temperature of. The method was evaluated according to ICH guidelines for the various validation parameters, such as linearity, accuracy, precision, LOD, LOQ, specificity, and Forced degradation studies. The results of intraday and inter-day validation (n = 3) showed the method to be efficient and the same was applied in the assay of lenalidomide in tablet formulation. In conclusion, this was a simple and effective method using HPLC to detect lenalidomide in tablet formulation, which may be useful for routine quality control analysis. Keywords: Lenalidomide, antineoplastic, Waters X-terra, Methanol, Acetonitrile, HPLC.

1190-1197

Authors: P. Sravan Kumar1 , Asish Bhaumik*2 , Mrinal Kanti Sarkar3 , Nibedita Roy3 , Mahendar Boddupally4

Abstract: The main aim and objective of the present research work was to extract the potential biomolecules present in the fruit seeds of Glycosmis pentaphylla L and to evaluate the in vitro and in vivo Hepatoprotective activity. The in vitro Hepatoprotective activity was carried out by DPPH assay. The IC50 value (50% inhibition) of the EEFSGP was found to be 212.96 µg/ml. The in-vivo Hepatoprotective activity was carried out by using albino rats. The results displayed that the elevated levels of SGOT, SGPT, ALP and Serum bilirubin were mainly due to CCl4 intoxication, reduced significantly (*P<0.05) in rats, after treatment with ethanolic extract of fruit seeds of Glycosmis pentaphylla (EEFSGP). Treatment with EEFSGP at a dose of 250 mg/kg b.w. decreased the SGOT, SGPT, ALP, Serum Bilirubin levels by 6.23% ns(non significantly), 28.96, 8.81, and 11.11%ns (non significantly) respectively, while a higher dose of 500 mg/kg b. wt. was more effective, causing a reduction of 25.02, 47.65, 24.09, and 27.35%. Silymarin used as standard showed a reduction of 55.09, 68.98, 57.46 and 35.04% receiving CCl4 alone. So depending upon the experimental data it was confirmed that the biochemical parameters of the group treated with ethanolic extract was significantly lower than the CCl4 treated group. Moreover the treatment with the extract significantly reduced the previously raised levels of AST, ALT, ALP and bilirubin in hepatotoxic rats. Histopathological investigation displayed that at both doses (250 mg/kg b.w. and 500 mg/kg b.w.), the EEFSGP was possessed moderate to good hepatoprotective activity, but at 500 mg/kg b.w. executed excellent hepatoprotective activity against CCl4 induced damaged hepatocytes. Key words : Biomolecules, DPPH assay, Intoxication, Hepatocyte, SGOT, SGPT, SALP.

1206-1215

Authors: M. Purushothaman*, B. Narasimha Rao, V.Viswanath, A. Sowmya latha

In the present work, an attempt has been made to develop controlled release tablets of Quetiapine fumarate by selecting different grades of HPMC and Ethyl cellulose as retarding polymers. All the formulations were prepared by direct compression method using 12mm punch on 8 station rotary tablet punching machine. The blend of all the formulations showed god flow properties such as angle of repose, bulk density, tapped density. The prepared tablets were shown good post compression parameters and they passed all the quality control evaluation parameters as per I.P limits. Among all the formulations F3 formulation showed maximum % drug release i.e., 97.3 % in 8 hours hence it is considered as optimized formulation. Whereas the formulations containing HPMCK100M showed more retarding with increasing concentration of polymer. The formulations with Guar gum were unable to produce the desired rug release pattern. Key Words: Quetiapine fumarate, HPMC K15M, HPMC K100 M, Guar gum, Controlled Release tablets.

1227-1239

Authors: Athiya Sulthana*1 , A. Ravi kiran1 , Suneetha Gowdu.R2 , K. Vanitha Prakash2

Background: Colon cancer is the most common cancer and fourth most frequent cause of cancer deaths worldwide. 1,2 dimethyl hydrazine (DMH) is a toxic environmental pollutant which as reported as a specific colon procarcinogen. Aberrant Crypt Foci (ACF) in the colonic mucosa have been hypothesized to represent precursor lesions of chemically induced colon cancer. Cassia absus has been traditionally claimed to be an anti tumor agent but no studies has been done so far. Based on the traditional claims, we presumed that Cassia absus as a potential anti-oxidant and anti-tumor agent, may show ameliorating effect on DMH induced colon cancer in mice. Objective: The objective of the present study was to evaluate the ameliorating effect of ethnolic extract of whole plant of Cassia absus on DMH induced colon cancer in mice. Method: 30 mice (either sex) of 10 weeks old were divided into five groups (n=6)Group I - served as normal control group received vehicle p.o. Group II- disease control group received DMH (20mg/kg s.c.) twice a week for 2 weeks. Group-III and IV – drug treated group received DMH (20 mg/kg sc.) twice a week for 2 weeks and Cassia absus (200 and 400 mg/kg, p.o.) for 7 weeks. Group V received DMH (20 mg/kg s.c.) twice a week for 2 weeks and 5- fluorouracil (20 mg\kg i.p.) once daily up to 2 weeks. During the treatment period body weights was measured at weekly intervals. At the end of seventh week all the animals were sacrificed and their colons were isolated for quantification of Aberrant Crypt Foci, Biochemical estimations (MDA, GSH, Catalase, SOD) and Histological studies. Results: Induction of colon cancer by DMH administration significantly decreased high incidence of ACF, aberrant crypts and crypt multiplicity along with decreased anti oxidant levels and increased colonic MDA levels. Administration of Cassia absus resulted in the decrease in the number of ACF and crypt multiplicity, antioxidant enzymes levels which reflect a favorable balance between potentially harmful oxidants and natural anti oxidants which may be attributed to the chemo preventive effect of Cassia absus. Conclusion: In conclusion, the present study suggests that treatment with ethnolic extract of whole plant of Cassia absusexerts beneficial effect in DMH induced colon cancer in mice probably due to their anti oxidant and anti tumor properties. Key words: 1,2 dimethyl hydrazine (DMH), Aberrant Crypt Foci, Cassia absus