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Effect Of Diode Laser When Used Alone Or In Combination With Various Irrigants On Root Canal Microbes-an In Vivo Study
Authors: Dr Anupama Kumari1, Dr. Kapil Loomba2, Dr Abhinav Singh3 ,Dr Piyush Chaudhary4, Dr Vivek Raj Gautam5
Number of views: 514
Objective: To evaluate the in vivo effect of diode laser when used alone or in combination with
various irrigants on root canal microbes
Methodology: A total of 25 patients in age group of 15-45 of distal canal of Permanent molars were
selected from patients who had visited to the Department of Conservative and Endodontic Dentistry,
Chandra Dental College and Hospital, Barabanki. Healthy and cooperative patients were selected
for this study. They were subsequently divided into five groups comprising of 5 patients each - In
Group I, only diode laser was used. In Group II, diode laser was used in combination with 5% sodium
hypochlorite solution. In Group III, diode laser was used in combination with 2% Chlorhexidine and
in Group IV, diode laser was used in combination with saline whereas Group V was treated with a
combination of Sodium hypochlorite, 2% Chlorhexidine and Saline solution. Before treatment and
after treatment microbial assessment (pathogen, colony count and aerobe/anaerobe typing) was
done. Data was analyzed using SPSS 21.0 software. Kruskal-Wallis and Wilcoxon signed rank tests
were used.
Results and Conclusion: Positivity rate for aerobes ranged from 60% to 100% and for anaerobes
60% to 80% in study groups. Staphyloccus aureus, Coagulase negative Staphylococcus,
Streptococcus and Bacillus spp. were the aerobic isolates. Enterococcus faecalis and Pseudomonas intermedia were the anaerobic isolates. Mean
reduction in aerobic colony count ranged from 73.92±6.36% (Group I) to 83.88±10.85% (Group V). Mean reduction in aerobic colony count ranged
from 70.43±24.08% (Group V) to 95.98±6.97% (Group II). Statistically there was no signfiicant difference among the groups. On evaluating the
extent of reduction in microbial count, the only significant reduction was observed in Groups IV and V for anaerobes (p=0.048). This preliminary
assessment showed that diode laser in combination with saline was comparable to a multimodal modality of NaOCl, 2%CHX and Saline combination.