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Comparative Evaluation of Antimicrobial Properties of Various Oral Preparations against Lactobacillus acidophilus – A Microbiological In-vitro Study
Authors: Dr. Binita Srivastava MDS, Professor& Head, Department of Pedodontics and Preventive Dentistry Santosh Dental College & Hospitals Ghaziabad, NCR, Delhi, India Dr. Parul Verma, MDS Dr. Nidhi Gupta MDS, Reader Dr. Rashi Singh MDS, Sr. Lecturer Dr. Dakshina Bisht MSc. (Microbiology), PhD, Professor& Head, Department Of Microbiology Santosh Medical College & Hospitals
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Aim: The present study was undertaken to evaluate various preparations – Chlorhexidine, Alum, Amla, Aloe Vera, Papaya and Probiotic for their
antimicrobial activity against Lactobacillus acidophilus and to compare the antimicrobial activity of these preparations against Lactobacillus acidophilus.
Materials And Method: Saliva samples of 30 children with nursing bottle caries or rampant caries were collected using sterile cotton swab, cultured onto the
Rogosa Agar culture plates and incubated aerobically for bacterial growth to become appreciated. Agar well diffusion method was employed for antimicrobial
susceptibility on Mueller Hinton agar plates. Crude form of extracts was used. Antimicrobial activity of the extracts was determined by measuring the mean
zones of inhibition in mm.
Results: 0.2% Chlorhexidine showed the highest antimicrobial activity against Lactobacillus acidophilus followed by alum and amla extracts. Probiotics, aloe
vera and papaya extracts were found to be ineffective against the bacteria.
Conclusion: According to the result it was concluded that oral environment could be stabilized by using these natural extracts as mouthwashes where alum
proved to be most effective against the bacteria.