Quality Comparison of Decellularized Omentum Prepared by Different Protocols for Tissue Engineering Applications
Authors: Khatereh Fazelian-Dehkordi; Sayed Fakhroddin Mesbah Ardekani; Tahereh Talaei-Khozani
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Objective: Decellularized greater omentum (GOM) is a good extracellular matrix (ECM) source for regenerative
medicine applications. The aim of the current study was to compare the efficiency of three protocols for sheep GOM
decellularization based on sufficient DNA depletion and ECM content retention for tissue engineering application.
Materials and Methods: In this experimental study, in the first protocol, low concentrations of sodium dodecyl sulfate
(SDS 1%), hexane, acetone, ethylenediaminetetraacetic acid (EDTA), and ethanol were used. In the second one, a
high concentration of SDS (4%) and ethanol, and in the last one sodium lauryl ether sulfate (SLES 1%) were used to
decellularize the GOM. To evaluate the quality of scaffold prepared with various protocols, histochemical staining, DNA,
and glycosaminoglycan (GAGs) quantification, scanning electron microscopy (SEM), Raman confocal microscopy,
Bradford assay, and ELISA were performed.
Results: A comparison of DNA content showed that SDS-based protocols omitted DNA more efficiently than the SLESbased
protocol. Histochemical staining showed that all protocols preserved the neutral carbohydrates, collagen, and
elastic fibers; however, the SLES-based protocol removed the lipid droplets better than the SDS-based protocols.
Although SEM images showed that all protocols preserved the ECM architecture, Raman microscopy, GAGs
quantification, total protein, and vascular endothelial growth factor (VEGF) assessments revealed that SDS 1%
preserved ECM more efficiently than the others.
Conclusion: The SDS 1% can be considered a superior protocol for decellularizing GOM in tissue engineering