International Journal of Medicine and Medical Research

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Authors: N. V. Pasyechko, V. M. Kulchinska, L. V. Radetska, L. V. Naumova, I. V. Smachylo, A. O. Bob, I. P. Savchenko, N. I. Yarema, M. Y. Havryliuk, A. I. Balaban, O. O. Bob, M. A. Dzhula, T. I. Krytskyi

OPEN JOURNAL SYSTEMS Journal Help Share it with: Share USER You are logged in as... okolodnytska My Journals My Profile Log Out NOTIFICATIONS View (152 new) Manage JOURNAL CONTENT Search Search Scope Search Browse By Issue By Author By Title Other Journals FONT SIZE Make font size smallerMake font size defaultMake font size larger INFORMATION For Readers For Authors For Librarians HOME ABOUT USER HOME SEARCH CURRENT ARCHIVES AUTHOR GUIDELINES SUBMISSION EDITORIAL BOARD INDEX & RANKING CONTACT Home > No 2 (2016) > Pasyechko VITAMIN D AND DIABETES MELLITUS TYPE 2 N. V. Pasyechko, V. M. Kulchinska, L. V. Radetska, L. V. Naumova, I. V. Smachylo, A. O. Bob, I. P. Savchenko, N. I. Yarema, M. Y. Havryliuk, A. I. Balaban, O. O. Bob, M. A. Dzhula, T. I. Krytskyi Abstract Background. As it is established that diabetes mellitus causes metabolic disturbances of all types (first of all because of carbohydrate metabolism), affection of blood vessels, nerves, different organs and tissues. However, the influence of DM on the level of microelements is still not investigated enough. Despite the sufficient variety of medicaments, compensation of diabetes mellitus is achieved in less than 30% of patients. For this reason, the search of new ways of treatment which are aimed at the improvement of carbohydrate metabolism is an urgent issue. Objective. The research was aimed to reveal the deficiency of 25-hydroxyvitamin D3 [25(OH)D3] and its correlation with carbohydrate metabolism. Methods. Thirty five patients with diabetes mellitus Type 2 aged 55-74 with illness duration 2-4 years were examined. The control group included 35 healthy people of the same age and sex. Levels of 25-hydroxyvitamin D3 [25(OH)D3] were tested by means of radioimmunoassay. The level of glycated haemoglobin was tested by means of liquid chromatography. Results. Correlation of the level of vitamin D with the degree of diabetes mellitus Type 2 compensation has been detected. Conclusions. A clear tendency to the improvement of diabetes mellitus Type 2 compensation after medication correction with vitamin D was evidenced.

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Authors: V. V. Koptiukh, I. Y. Galaychuk

Background. It is established that granulation wound has uneven bumpy surface and modern electric dermatomes cut skin with a smooth internal surface. Therefore there is no tightly contact between the smooth surface of a graft and granulation tissue. Objective. The method of harvesting by split-skin grafts with variable thickness (embossed) is proposed. Methods. An embossed skin graft with sinusoidal surface of inner side was obtained by modified dermatome with special technological plate. The embossed autografting of skin was applied in 16 patients (21-60 years old) with deep burns of 7-9% of body. For clinical comparison the part of granulating wounds in each patient was covered with embossed transplants and the other part with smooth split-thickness grafts. Results. During ultrasound Doppler examination of grafts on the fifth postop day the active blood flow in embossed transplants with 4-6 blood outbreaks and only 2-3 flashes in smooth graft were found. Clinically the embossed transplants were engraftment on 6-7th day. On 18-21st day the roughness of grafts surface disappeared. In two months the graft together with subcutaneous fatty tissue was taken in crease. Donor wound: on 14-15th day its surface was like “slate” but on 25-30th day the undulation of the re-epithelisation skin disappeared. Conclusions. The improvement of dermatome design with technological plate allows cutting scalloped split-skin grafts of varied thickness (embossed). Split-thickness embossed grafts combine anatomic and physiological features of skin cover granulation wound to adhere tightly because of the enlargement of area of their internal surface without retraction and/or secondary shrinkage. Donor wounds, after the harvesting of embossed skin grafts, contain the morphological elements of dermis which contribute to their complete spontaneous healing.

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Authors: N. V. Hasiuk

Background. The results of a comprehensive study of cytological features of epithelial differentiation processes in gums in patients with generalized periodontitis are presented in the article. Objective. The objects of clinical observations were patients with generalized periodontitis. The disease duration was 3-5 years. Methods. Material for the study was gums epithelium taken from a marginal area of gums in young adults, with generalized periodontitis. The epithelium was removed with a spatula, with subsequent transfer to a glass plate and drying in the open air for 3-5 minutes. The colouring of the material was done according to Romanovskyy-Himza, with a next microscopic and morphological analysis, taking into account the percentage of different forms of epithelial cells in normal and due to an age aspect. Results. One of cytological signs of periodontal pathology in young people is epithelial cells with signs of irritation. It should be noted that these cellular elements belong to the intermediate layer of cells that meet histological sections and ribbed surface layers are characterized by homogenization and vacuolization of cytoplasm as a manifestation of prolonged irritation by bacterial cell aggression. Conclusions. Analysis of cytograms proved general periodontitis in the examined patients, in absence of adequate therapy as automotive continuous process. Its components may regress under the influence of effective treatment and re-activate during exacerbation of the inflammatory process under the influence of pathogenic factors such as microbial, hygienic and immune.

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Authors: M. I. Maruschak, M. M. Khudobyak, I. Ya. Krynytska, I. V. Antonyshyn

Background. The injured with polytrauma are classified as extremely severe patients, as their emergency care and intensive care is followed by significant difficulties, frequent development of complications, high mortality and survivors disability. Objective. This study is aimed to evaluate the effect of lipid peroxidation in heart, lungs, liver and blood of rats in early and late periods of combined traumatic injury of chest and hips and to establish their influence on the development of multiple organ failure. Methods. The study was conducted on 70 adult male white nonlinear rats. It was determined the content of lipid hydroperoxides and concentration of TBA-active products in erythrocyte mass, heart, lungs and liver. Results. The data prove activation of free radical oxidation at the first day of post-traumatic period. Analysis of the data evidences the increase in of lipid hydroperoxides (HPL) rate in liver homogenate in group E1 in 1.5 times, in all subsequent periods of the research the increase in rate fluctuated within 1.9-2.0 times. In blood and heart the HPL rate increased twice in group E1, reached maximum in group E2 and gradually decreased till the end of the experiment, data exceeded the control group. The highest level of HPL was determined in lung tissues (r≤0.01). In the post traumatic period the highest TBA-AP was detected in lungs and liver. Conclusions. In case of simulated trauma (injury of chest with fractures of both hips) hyperactivation processes of free radical oxidation is observed in 1 day, reaching a peak in 7-14 days of post-traumatic period in blood, tissues, liver, heart and lungs if compared to the control group causing multiple organ failure.

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Authors: V. I. Denesyuk, O. V. Denesyuk, N. O. Muzyka

Background. According to the national registries of European countries and epidemiological studies, the prevalence of chronic heart failure (CHF) among adults is 2,0-5,0%, and increases due to age, in people aged over 70 years old it is 10,0-20,0%. Objective. To find out the specific features of remodeling of the left atrium and change of vasodilation factors in ischemic heart failure with reduced and preserved left ventricular ejection fraction and to establish correlation relationships. Methods. A full clinical examination of 153 patients with CHF (105 men and 48 women) was conducted to achieve this objective. The surveyed patients underwent clinical examinations; spectrophotometric parameters: quantification of markers of vasodilation, metabolites of monoxide nitrogen – nitrates and nitrites with Gris reagent; content of endothelial nitric oxide synthase (eNOS) in serum - ELISA for the set of Nitric Oxide Synthase 3, Endothelial (NOS3) Human ELISA Kit (Cloud-Clone Corp, USA). Electrocardiographic (ECG) examination was conducted in 12 standard conventional leads on electrocardiograph by the Hungarian production Heart Screen 112 D. Results. The 1st group of the examined patients with reduced LV EF prevails III (significant) degree LA dilatation in 33 (70.21%) cases, II (moderate) degree of LA dilatation was determined in 14 (29.78%), and I (initial) degree was not defined at all. In the 2nd group of the patients with preserved LV EF mainly the II degree of LA dilatation was determined in 44 (44.51%) cases, and decreased LA dilation in 39 (36.79%) cases (p<0.01), and III degree of LA dilation was defined in 23 (21.69%) cases (p<0.01). In patients with stable coronary heart disease, complicated by heart failure with reduced LV EF and II degree of LA dilatation, eNOS levels in the serum was 449.00±39.91 pg/ml, whereas in patients with stable coronary heart disease, complicated by heart failure with preserved LV EF and II stage of LA dilatation – 673.56±50.98 pg/ml (p<0.01). At III stage of LA dilatation in patients of the 1st group level eNOS was 344.20±51.98 pg/ml in the patients of the 2nd group – 616.90±36.49 pg/ml (p<0.01). At the same degree and with LA dilation in the patients of the 2nd group eNOS was 750.27±99.85 pg/ml. Conclusions. The structural and functional changes of the left atrium and changing factors of vasodilation in patients with stable coronary artery disease of II-III functional classes complicated by heart failure of I-III functional classes are studied. It is established that in the examined patients with stable coronary heart disease complicated by heart failure with reduced left ventricular ejection fraction mainly III (significant) degree of dilatation of the left atrium was determined, while in patients with stable coronary heart disease, complicated by heart failure with preserved left ventricular ejection fraction mainly II (moderate) degree of dilation of the left atrium was determined. In comparison with the results of research among the patients with stable coronary heart disease, complicated by heart failure with reduced left ventricular ejection fraction, and a group of patients with preserved left ventricular ejection fraction, it was determined a significant decrease in eNOS, nitrites, total amount of nitrites and nitrates.

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Authors: I. R. Bekus, M. V. Kyryliv, I. B. Ivanusa, O. B. Furka, I. Ya. Krynytska, M. I. Marushchak

Background. Lipid metabolism disorders in the organism affected by environmental pollutants, including poisoning with cadmium and lead salts are of topical matter nowadays. Objective. The study was aimed to examine biochemical features of lipid metabolism in rats subjected to toxic damage by lead and cadmium salts and treated with carnitine chloride and Algigel. Methods. Experiments were carried out on white mature outbred male rats weighing 180-200 g. To cause the toxic damage the animals were administered with aqueous solution of cadmium chloride and lead acetate daily for the period of 30 days using intra-gastric lavage. The indices of lipid metabolism were detected by biochemical methods. Results. In animals treated with cadmium chloride and lead acetate the following changes were observed: HDL-cholesterol concentrations significantly decreased, resulting in 87% of the levels in the intact animals on the third day, 84% on the fifth and 80% on the seventh day. Conversely, concentrations of HDL-cholesterol and VLDL-cholesterol significantly increased during the experiment. Respectively, the ratios for HDL-cholesterol are 240%, 352%, and 388%; and for VLDL-cholesterol 108%, 116%, and 132%. Conclusions. Lipids profile of the rats displayed changes in the levels of cholesterol, triglycerides and lipoproteins of low, high and very low density.

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Authors: Iu. M. Ordynskyi, O. V. Denefil

Background. Cardiovascular morbidity is a topical issue; stress is an essential contributing factor. Pathogenic links in damaging stress impact on the animal units of different reactivity is promising in disease prevention and development of individual correction methods. Objective. Stress-induced development of cardiovascular pathology is undeniable, the stress impact depending on individual systemic response, age and sex. Methods. 96 hypoxia high- and low-resistant (HR and LR, respectively) Wistar rats aged 5.5-6 months were used in experiments. Changes of lipid peroxidation processes, as well as protein oxidative modification, nitrite anion content and the indices of antioxidant defence under immobilizing stress were studied in the research. Results. Immobilizing stress causes the development of oxidative and carbonyl stress in HR and LR rats that is more pronounced in LR group; and the activation of antioxidant defence system. In males, stress development is concomitant with increased catalase activity as well as that of blood peroxidase, ceruloplasmin and reduced glutathione content, whereas increased catalase and ceruloplasmin activity has been found in HR females, and that of superoxide dismutase and ceruloplasmin – in LR group. As compared with the females, more intensive oxidative and nitroxidative stress, protein oxidative modification, and stress-related accumulation of circulating immune complexes have been found in the males. Conclusion. The most intensive oxidative and nitroxidative stress, protein oxidative modification, and stress-related accumulation of circulating immune complexes have been found in the hypoxia low-resistant males.