International Journal of Applied Medical and Biological Research

4

Authors: Rugaia Mohammed Abdul-Gader Algazoui, Meraj Bano, Abdulhafeez Khan

We evaluated the diagnostic performance of microscopically examination of staining techniques (Modified Ziehl-Neelsen and Auramine-Rhodamine) and Coproantigen Enzyme Linked Immunosorbent Assay (Copro-ELISA) for cryptosporidiosis diagnosis. Copro-ELISA appeared to be most sensitive than staining techniques. The commercial Copro-ELISA and Auramine-Rhodamine proved to be valuable diagnostic tools for Cryptosporidium infection.

5

Authors: Somia A.Al-Ghani, Salma M. Aljad, Omar M. Abukhres, Ibrahim A . Mukhtar, Ali F. Hawad, Hussein Al-Rasheed and Aisha M. A. Shahlol

This study was undertaken to examine the prevalence of Cytomegalovirus in a group of women in the area of Brack Al-Shati Serum samples were collected from 180 pregnant women attending Brack General Hospital for consultation and treatment. Women chosen for the study were in different gestation weeks. Medical history and relevant information were recorded. Samples were examined serologically by Enzyme Linked Immunosorbent Assay (ELISA) to measure the titer of immunoglobulin G (IgG) against Cytomegalovirus. One hundred and seventy two (95.6%) of samples were positive for the Cytomegalovirus, with titers range between 1.6-58.17 IU/ml. Conclusion: CMV was found to be quite common in this area. 20% of CMV positive women suffer from complications such as abortions, defected babies etc., and this probably due the infection with CMV.

7

Authors: Hind M. Khalfallah, Omar M. Abukhres, Ali F. Hawad, Ibrahim A.Mukhtar, Hussien A. Alrasheed

The objectives of this study were to assess the bacterial content of air conditioning and indoor air of some hospitals in the south of Libya. This study was conducted in a 2nd of March hospital and Brack general hospital in southern Libya. Samples were collected from indoor air, and from filters, cooling coils, and water drainage basins of air conditioning units in those hospitals. Bacterial colonies were counted and identified characteristics of the bacteria on blood agar and MacConkey agar, also identified bacterial species. Bacterial numbers in the samples taken from 2nd March hospital formed 56.57% of the total indoor air samples, 79.9% of the total filters samples, 72.07% of the total coils samples, and 54.63% of the total water samples. The bacterial numbers in samples taken from a Brack general hospital contained 43.43% of the total indoor air samples, 20.1% of the total filter samples, 27.93% of the total coils samples, and 45.37% of the total water samples. Hemolytic bacteria were present in smaller numbers on blood agar than non-hemolytic bacteria, and were presenting more non-fermented at a higher rate than fermented bacteria on MacConkey. The bacterial species that were identified included some species of pathogenic bacteria as S.aureus, which existed in the various samples, K. pneumonae, Bacillus and Pseudomonas, those species involved in nosocomial infections. Conclusion: The occurrence of high bacterial numbers on parts of air-conditioning followed by its spread to the internal environment which constitutes a health risk to people exposed to it especially if this environment are the hospitals where patients with various injuries.