Annales Bogorienses

1-5

Authors: Amy Estiati, Syamsidah Rahmawati, Dwi Astuti, Inez H.S Loedin

A cry1B synthetic gene of Bacillus thuringiensis has been used for the transformation of the javanica rice plants cv. Rojolele confer resistance to an important pest, yellow stem borer (Scirpophaga incertulas). Embryogenic callus were co-cultivated with the EHA 105 strain of Agrobacterium tumefaciens harbouring binary vector CAMB IA 130 I containing cry / B gene under the control of wound inducible gene promoter (mpi), hygromycin resistance gene (hpt) as a selectable marker and intron-containing β-glucuronidase (gus - intron) gene al) a reporter gene driven by CaMV35S promoter. Previously. our histochemical assay and P R analyses. had proved th integration of cry1B gene into the genome of rice plants at first generation . However, the existence of the gene should remain table throughout generation. In this study, the presence of the cry1B transgene in rice transgenic plants at second generation was confirmed by Polymerase Chain Reaction (PCR) . Insertion of the cry1B gene in the genome of PCR positive plants was verified by Southern blot analysis is and showed that integration of cry1 B into the genomic DNA of javanica rice plants cv. Rojolele. An effective resistance of transgenic plants against stem borer was verified in bioassays.

15-21

Authors: Andi Utama, Hiroyuki shimizu

The role of motif n (DEAR AsPw-GlulK,,-Ala.,.7-Hi 1~~ ) of Japanese encephalitis virus (JEV) NS3 protein on RNA binding activity was studied. A point mutation was introduced to the motif and the RNA binding activity of each mutant protein was analyzed. Truncated form of each protein with a His-tag was expressed m Escherichia coli BL2l(DE3) pLysS and purified by metal affinity resin. Asp-285 - and Glu-286 was respectively substituted with Ala, AJa-287 was replaced by Cys.,Gly. or ser. His-288 was mutated to other 19 amino acids. In total. 24 mutant proteins were produced and analyzed. AL results, all mutants showed quite similar RNA binding activity. Indicating that motif II of JEY NS3 is not related to RNA binding activity. The same finding was reported for hepatitis C virus c (Hey) NS3 protein, suggesting the similar structure of NS3 protein in flavivirus .

31-39

Authors: Endang sukara

food security is one of the essential needs which can never be ignored by any society, including in particular Indonesia. as the world population is expected to rise to around 10 billion marked by 2025. The problem is that the agriculture in developed and developing countries that follow the path of developed countries experiences create · a range of serious environmental degradation. An intensive agricultural production through the use of pesticide and herbicides, forest clearing and continued use of land resources for the agricultural purposes are not sustainable practice . In consequences, degradation of ecosystem, oil erosion. and low productivity of land, poor water quality and lost of biological diversity (including microbial and genetic resources) occurred at alarming stale. Meanwhile, the efforts in addressing the above situation is traditionally focused on plant phenotypes (breeding. plant genetic engineering etc .) but the important role of microbial communities that interact with plants to influence plant health and productivity has been largely ignored. The interaction of microbes with plants and its relevant to soil fertility and productivity, the bio-control properties of microbes and challenges toward sustainable agriculture is highlighted.