INTERNATIONAL RESEARCH JOURNAL OF SCIENCE AND ENGINEERING

65-75

Authors: Kulkarni Keya and Sundarrajan Priya

Cytochrome C is an essential and ubiquitous protein. It is highly conserved across the spectrum of species and hence is used to study cladistics. In this paper, analysis of cytochrome C using bioinformatics tools has been carried out, in order to find the evolutionary relationship between species. The amino acid sequences of cytochrome C were used to establish the phylogenetic relationships of species. The phylogeny inferred from the sequence alignment of cytochrome C was confirmed by comparing its structure and function. The protein was found to be functionally redundant. From the analysis cytochrome C, it appears that two different organisms which have the same or even similar protein sequences are genealogically related. The homologous similarities of cytochrome C are suggestive of common ancestry.

85-89

Authors: Chavhan Dasharath M, Patil Shrikant S and Khandekar Snehal R

Lemon juice containing citric acid acts as a natural acid catalyst in Acetylation of amines and hydroxyacids such as salicylic acid. Acetylation of different amines and salicylic acid were carried out by using lemon juice and acetic anhydride as an acetylating agent, reaction proceeds under normal reaction condition with the formation of product in high yield. The reactions were carried out with magnetic stirring at room temperature and under solvent free condition. The present methodology illustrates the efficient acetylation of primary amines and Salicylic acids by lemon juice by means of acetic anhydrides under weakly acidic condition at room temperature.