147-150
Apoptosis is induced by curcumin in synergism with 5FU in human colorectal cancer cell line.
Authors: Ramin Ataee, Ali Asghar Ahmadi, Mohammad Shadifar, Amin Ataie, Cathy Vaillancourt, Majid Jafari-Sabet, Talal Oufkir.
Number of views: 519
Sci-Afric Journal of Scientific Issues, Research and Essays Vol. 2 (4), Pp. 147-150, April, 2014. © Sci-Afric Journal
Research Paper
Apoptosis is induced by curcumin in synergism with 5FU in human colorectal cancer cell line.
*Ramin Ataee1, 2, Ali Asghar Ahmadi3, Mohammad Shadifar3, Amin Ataie4, Cathy Vaillancourt5, Majid Jafari-Sabet6, Talal Oufkir5
1. Talasemia research center, Mazandaran University of Medical Sciences, Sari, Iran.
2. Pharmaceutical sciences research center, Mazandaran University of Medical Sciences, Sari, Iran.
3. North research center-Pasteur institute of Iran, Amol, Mazandaran, Iran.
4. Department of Pharmacology, Babol University of Medical Sciences, Babol, Iran.
5. INRS-Institut Armand-Frappier, Université du Québec, Laval, QC, Canada.
6. Department of Pharmacology, AJA University of Medical Sciences, Tehran, Iran.
*Correspondence Author E-mail: ramin_ataee@yahoo.com
Accepted March 6th, 2014
Abstract
Background: Curcumin, a phenolic derived from the rhizome of the Curcuma longa linn (Zingi-beraceae), has been recognized as a promising anti-cancer drug due to its multiple properties, including anti-inflammatory,antioxidant and anti-angiogenic. Also some evidences show its' anti-cancer effect in some malignancies as in colon, prostate and breast cancer. To show better, the role of curcumin in colon cancer and apoptosis in comparison with 5flurouracil (5-FU) we have planned this project.
Material and methods: For Proliferation test we used MTT assay and for apoptosis study we used TUNEL assay. Also 5FU has been provided as positive control
Results: Our experiment has shown that curcumin, in a dose-dependent manner, reduces cell viability and increases apoptosis in HT29, SW480 and SW742 colorectal cancer cell lines as analyzed by MTT and TUNEL assay, respectively. Moreover, combined with 5FU synergistically increased the number of hypodiploid cells and DNA fragmentation and decreased cell viability in all colorectal cancer cell lines. This study demonstrates that adding curcumin to 5FU have a synergic anti-mitogenic and apoptotic effect on colorectal cancer cell lines. These results suggest a potential use of serotonin 5-HT2A receptor antagonist in co-treatment with curcumin in colon cancer therapy.
Keywords: Curcumin, Colon Cancer, 5FU, MTT assay, Apoptosis.