Environment&Health (Dovkillia ta zdorovia)

4-8

Authors: КАРЛОВА О.О., ЯВОРОВСЬКИЙ О.П.

Objective. We determined a character of the content of nitrogen oxide and its metabolites in blood depending on the lead concentration in the blood of electricians. Materials and methods. Monitoring result analysis by the index of lead concentration in the of 146 electricians of the PJSC Kabelnye Seti for repair and installation of cable net demonstrated that xenobiotic concentration in blood were determined in the limits of 1.34 -2.31 µmol/l, in 51 persons it exceeded an upper limit of the allowable values (1.93 µmol/l).Those levels are dangerous for the workers’ health. The symptoms of neurasthenic and astheno-vegetative syndromes prevailed in the clinical finding of the workers, just those persons made up the 1-st group. In 95 of them (65%) a content of lead in blood was at the allowable level (0.96-1.93 µmol/l). According to the data by I.N. Trakhteberg et al. (1999), it pointed to the presence of the metal in the organism. Taking into account the features of clinical manifestations of the astheno-vegetative and neurasthenic syndromes, the workers with the allowable content of lead were divided into two groups: the 2-nd group constituted 46 persons with the clinical symptoms of the severe astheno-vegetative syndrome; the 3-rd group made up 49 workers with the separate symptoms of astheno-vegetative syndroms in their clinical finding. Lead concentration in the blood of the 1-st group made up 2.12 ± 0.012 µmol/l, the 2-nd group – 1.92 ± 0.013 µmol/l, the 3-rd group – 1.72 ± 0.028 µmol/l. In each group of the examined persons the level of lead in blood differed authentically between each other (p<0.05). The control group consisted of 57 workers who didn’t have contact with lead according to their occupational anamnesis. Results. We presented the results of the examination of the workers exposed to lead. The revealed excess of the content of nitrates-nitrites in the blood serum of all examined workers was accompanied with the accumulation of the content of S-nitrozotioles (RS--NO +) and dinitrosyl iron complexes (RS-)2Fe + (NO+)2). Determination of the content of nitrites-nitrates (NO2-/NO3-) and their stable metabolites (S-nitrozotiol (RS--NO +) and dinitrozyl iron complexes (RS-)2Fe + (NO+)2) in serum was recommended as the markers of early diagnosis of endothelium dysfunction at lead intoxication.

13-16

Authors: РОГАЧ І.М., ЄРЕМ Т.В., ЄРЕМ Х.В.

Objective. We studied a content of fluor trace element in soil-foodstuffs-human chain for the revealing of the localities and the contingents of people with a deficit or a toxic concentration of this element. Materials and methods. We studied the soil samples taken from the lands of Transcarpathian region of all 13 administrative regions. Samples of vegetable mixture for study were taken in the local farms. We performed the analysis of water supply sources on the content of fluor of all regions of the region. Results. Ultra low fluor concentration was determined in the environmental objects (soil, drinking water) and in food intakes of the residents of the Transcarpathian region. Recommended and minimum allowable values (according to the physiological standards) of the consumption of fluor with a daily sets of the foodstuffs were calculated, taking into account a variability of factual nutrition, and for the representative prognostication of the population’s provision of fluor. Conclusions. Environmental objects of the Transcarpathian oblast show the ultralow content of the fluor trace element, it causes a hypofluorosis of the biosphere on the studied territory. We noted a fluor deficit in the organisms of the local residents because a content of fluor in the organism depended on the level of the intake and closely connected with the dissemination of the element in the biosphere of the region. We determined that index of the caries prevalence in the local residents made up 80-90%; CFE index (caries+ filling +extraction) made up 4.5-5.0 at average index in Ukraine of 2.75.

22-25

Authors: ШЕНЦОВА М.О., СУРМАШЕВА О.В., НІКОНОВА Н.О., САХНЮК О.М., НАСТОЯЩА Н.І.

Actuality of the work lies in the significance of the standardization of probiotic preparations. The standard samples of probiotic microorganisms are used for their standardization. Determination of standard samples’ safety is a compulsory condition. Materials and methods. We used generally accepted methods of the assessment of the microorganisms’ safety. Assessment of the safety of probiotic strains was conducted in acute and chronic experiments of the toxicity by the presence of lethal effect and change of the indices characterizing the functional state of the organism and separate organs of the experimental animals – mouse and rats. Results. During the testing of the strains B. bifidum and E. faecium at their single introduction into the organism of the animals (intraperitoneal, intragastric and intravenous) we failed to determine a main parameter of toxicity - an indicator of median lethal dose LD50. At the multiple introduction of the suspensions of probiotic strains B. bifidum and E. faecium into the organism the structural and functional changes in tissues and organs of experimental animals were not fixed, it indicates the strains’ safety. Thus, a safety of the microbial populations in the created standard samples B. bifidum and E. faecium was demonstrated in the models of acute and chronic experiments.

30-35

Authors: Махнюк В. М., Павленко Н. П., Фещенко К. Д., Могильний С.М.

Objective. We substantiated a significance of the hygienic indices of insolation and natural lighting at the design stage of residential complexes with the integrated educational institutions and other commercial facilities (social and domestic, administrative, public utilities, and others.) under conditions of the compact residential building. Methods. We used sanitary-and-hygienic, analytical methods, methods of sanitary-epidemiological examination, substantiation of proposals, and hygienic recommendations. Subject of inquiry. The results of the scientific sanitary-epidemiological examination of residential and recreational complex with the integrated and attached children’s preschool institution and commercial facilities (Kyiv) were the subject of inquiry in a given work. Results. In the presented project a placement of integrated and attached preschool institution and a number of the commercial facilities (social and domestic, administrative, public utilities, and others.) in the united planning space was provided by the architectural solutions. National legislation does not provide a placement of the above-mentioned objects in the united planning space of the residential complex. There is no experience of the functioning of the analogues existing objects both in Ukraine and abroad as well. The non-standard architectural planning solutions were applied for the supply of the standard levels of insolation and natural lighting of the classrooms and bedrooms oriented to the north-east, north and south facades. The height of these premises in the united building space is allowed from 3 m up to 7.6 m. As a result, the roof of the first floor has a "zig-zag" form on the south facade. The introduction of such innovations allowed a design of the additional upper side window on the wall of the south facade (7.6 m height) and a providing of the standard insolation and natural lighting in all classrooms irrespective of the facades’ orientation. Placement of the multifunctional objects on the 1st-2nd floors in the united building space and in the united outbuilding territory of the residential complex completely eliminates the possibility of providing the different functional areas of the adjacent territory. Such a placement does not provide a sanitary and epidemiological welfare for all groups of the population. The hygienic indices of insolation and natural lighting were substantiated to be the determinative indices of optimal planning organization of the territory of residential complexes under conditions of compact residential building.

40-43

Authors: ШЕВЦОВА Т.В., ШИДЛОВСЬКИЙ А.Ю., КОЗАК-ВОЛОШАНЕНКО Ю.М., ГВОЗДЕЦЬКИЙ В.А.

In the work hearing characteristics of noise in intensity and ego frequances characteristics of patients in comparative plan with beginning sensoneural hearing vascular and noise (Rabochie Coal Industry) origin. State of the problem: to make comparative analysis of patients with sensonevral hearing and vascular genesis noise on development, ushnoy noise intensity. Materials and methods. That was the 90 Study conducted of patients with primary vascular genesis and 49 - noise, availability subjective hearing noise. Audiometric examination was performed on clinical audiometer AC-40 company «Interacoustics» (Denmark) in soundproof chamber, where the background noise does not exceed 30 dB. Specified audiometer allows to investigate auditory sensitivity to tones from 0.125 to 8 kHz frequencies and 10; 11.2; 12.5; 14 i 16 kHz in air. Results. Among Coal Industry of employees noise observed in workers (58.8%), less - in another workers (53.8%) and else less (42.1%) of workers. Conclusion. Among Coal Industry of employees noise observed in of workers storm, something obviously, can load and vibration. Data mogut for Understanding pathogenesis of sensonevral hearing origin. With availability in noise workers and Coal Industry of abuse of patients with sensonevralnoy tuhouhostyu the study auditory function not only in (0,125-8 kHz), but in an enlarged and (9-16 kHz) frequency bands, something causes appearance sensonevral hearing.

51-56

Authors: ПЛАСТУНОВ Б.А., КОВАЛІВ М.О.

Aim: to assess according to accepted methods the physical and intellectual development of HMEI entrants. Results. It was established the morphofunctional development of HMEI entrants depending on sex, age, place of residence and nutritional status, including iodine and vitamin C supply and trans-alimentary lead intake. Approaches to optimize their nutritional status were identified.

61-63

Authors: СВЄТЛОВА О.Д.

We analyzed a morbid affection of the urban schoolchildren in the central region of Ukraine for the last 28 years. A significant increase of the level of the morbid affection among the middle school age children was revealed. For the studied period of time it grew more than twice as large from (425.98 ± 3.45)‰ in 1986 to (995.82 ± 0.63)‰ in 2013. According to our prognostication the morbid affection level among schoolchildren will increase in future and it will make up (1065.52 ± 61.59)-(1087.53 ± 64.86)‰ in 2014-2015. It is stipulated by the next increase of the proportion of diseases and functional disorders of musculoskeletal system, diseases of eyes and adnexa, digestive system, circulatory and nervous systems. For a decrease of these tendencies the additional health-improvement and rehabilitation measures should be performed.

68-72

Authors: СТАДНИК Л.Л., НОСИК О.В., ШАЛЬОПА О.Ю.

Objective. We studied the collective effective dose of the Ukrainian population and its distribution in different types of X-ray diagnostic examinations, assessed the irradiation doses of "standard" patients at the most wide spread X-ray examnations, and established the recommended diagnostic levels. Materials and methods. We used sociological (questionnaires), dosimetric (thermoluminescent, ionization), statistical methods. The evaluation of the frequency of X-ray examinations was carried out by the results of the survey of X-ray diagnostic radiological services of Ukraine. The collective effective dose of the population of medical X-ray exposure was defined. About 4,000 measurements of the entrance surface doses of the patients for 9 of the most mass types of radiographic examinations and chest fluorography by the method of thermoluminescence dosimetry were performed. The distribution of doses for each of the selected types of examination was studied, the values of the third quartiles of dose distributions were determined. A comparison of direct and indirect methods of the evaluation of the entrance surface doses of the patients was performed. Results and discussion. By the results of the performed survey of the state of X-ray diagnosis services we studied a structure of X-ray diagnostic examinations carried out in 2013 in Ukraine and assessed a collective dose of the of the population of the country which made up 32578.2 man-Sv. We established that film chest fluorography (37.2%) and radiography (31.4%) made the greatest contribution into the collective effective dose of medical exposure. The distribution of doses was studied by the results of the measurements of the entrance surface doses of the patients at chest radiography, cervical, thoracic, lumbar spine (in two projections), pelvis and chest fluorography, average doses’ meanings and the third quartiles were determined, they can be accepted as national diagnostic reference the selected types of investigations according to the diagnostic recommended levels (DRLs). Obtained meanings of diagnostic recommended levels were established to increase a reference diagnostic levels 1.3-2.0-fold by the selected types of the examinations according to the IAEA standards (BSS-115). Optimization of medical diagnostic exposure in Ukraine may be possible through the implementation of national DRLs for X-ray diagnostic examinations, performance of dose control of the “standard” patients and their comparison with the DRLs, revealing and removal of the reasons of the excess of doses by the quality control of the parameters of X-ray units, technology of the performance of X-ray diagnostic procedures.

77-79

Authors: ЯВОРОВСЬКИЙ О.П., ЦЕХМІСТЕР Я.В., САХАРЧУК І.М., ЄЗІКЯН Г.М.

The foundation history of the Kiev medical institute is investigated based on the archive and documentary, scientific and publicist materials analysis. It`s demonstrated that his foundation process lasted more than four years and passed in his development process six periods, which had been changed depending on those or other political forces governments. It is concluded that the Kiev medical institute is founded in 1920 as a higher national medical foundation became assignee not only St. Vladimir imperator`s university of the medical faculty as previously have thought, but also the medical faculty of the Ukrainian national university.

4-9

Authors: МАКОВЕЦЬКА Л.І., ГАНЖА О.Б., ГЛАВІН О.А., ДРУЖИНА М.О., ПАШКЕВИЧ Я.І., МИХАЙЛЕНКО В.М.

The aim of the study was to investigate the effect of prolonged inhalation exposure of exogenous NO on violation of free radicals and metabolic processes in an organism, which can lead to cancer. Materials and methods. The study has been carried out on male C57Bl/6 mice, 6 weeks old, weighing 20-25 g. The experimental animals were exposed to NO in inhalation chamber during 10 days for 14 hours per day with NO concentration of 50 mg/m3 of air. Free radical homeostasis assessed by the level of superoxide anion radical generation, the content of reduced glutathione, the chemiluminescence light sum, enzymatic activity of xanthine oxidoreductase and catalase. Results. It was shown that long-term inhalation of exogenous nitrogen oxides was accompanied by an increased rate of superoxide anion-radical generation in hepatocytes, decreased level of reduced glutathione, stimulation of xanthine oxidoreductase activity in the liver and decreased catalase activity in the blood of C57Bl/6 mice. Overall upward trend of chemiluminescence was registered that indicates the predominance of prooxidant processes in prooxidant-antioxidant balance in the blood. Thus, prolonged exposure of exogenous nitrogen oxides stimulated the oxidative stress development in mice, which was characterized by the intensification of the superoxide anion-radical generation, inhibition of catalase activity of antioxidant system and the shift of pro- antioxidant balance toward prooxidant processes that can lead to DNA damage and the development of genetic instability in cells.

14-19

Authors: ФЕДОРЕНКО В.І., КІЦУЛА Л.М.

Objective. We performed a comparative evaluation of anthropometric parameters (body length and weight, chest circumference) in 7-17 years-old schoolchildren in Western and Eastern regions of Ukraine for the establishment of the territorial peculiarities of their physical development as an example of the cities of Lviv and Luhansk. Materials and Methods. The anthropometric indices, obtained concurrently, and developed scales of physical development regression among 7-17 years-old schoolchildren in the cities of Lviv and Luhansk were the materials for the investigation. A reliability of the differences between the average values of body length and weight, and chest circumference of age-and-sex groups of schoolchildren of both cities was determined by the Student t-criterion. The peculiarities of harmonious physical development were considered on the base of the calculated body mass indices for each age-and-sex groups of schoolchildren and comparison of their values with the standards offered by Platonova A.H. (2012). Results. Significant differences between body length and weight, chest circumference were established among 7-17 years-old girls and boys in Lviv and Luhansk . Anthropometric indices by absolute values of the Lviv schoolchildren of all age–and-sex groups were higher than those of the schoolchildren of Luhansk. The Luhansk 16-years-old boys with a body length higher than in the Lviv schoolchildren of the same age and also 8-9-years-old boys and girls from Luhansk, whose weight exceeded the body weight in the Lviv schoolchildren of the same age, were the exception. Irregularity of harmonious physical development, disharmonious of physical development due to overweight in the separate age-and-sex groups of schoolchildren and excessive body weight of the Lviv elder schoolgirls were observed. The differences between physical development indices of schoolchildren from different regions can mainly depend on environmental pollution, inadequate nutrition and other factors which indicate the necessity of further study of causal-consequent relationships between indices of physical development and the affected factors, and also the turnover of the regional standards of physical development for its further evaluation in dynamics.

24-29

Authors: П’ЯТНИЦЯ-ГОРПИНЧЕНКО Н.К.

Purpose of the study was to generate the results of scientific studies, concerning algorithms for assessing the quality of workplace air, definition of priority problems, causing the need of further studying. Materials and methods. An analytical review of the scientific papers has been made, including abstract databases “PubMed” [http:www.ncbi.nlm.nih.gov/pubmed]. Results of studies and discussion. Monitoring of the industrial aerosols levels is the only way to relate occupational exposures to the revealed pathology. Published sources testify that a lot of issues related to the description of the air condition in the workplace environment require further study. Primarily, it concerns harmonization of the existing dust screening techniques through the development and adoption of appropriate national standards so that to obtain representative results of the research. Conclusion. The data set forth in the article allow to improve accomplishment of the sanitary inspection of the air environment of production facilities to ensure safe working conditions and prevent formation of the occupational diseases of the respiratory tracts.

33-36

Authors: ДУМКА І.В., ТКАЧИШИН В.С., ТКАЧИШИНА Н.Ю.

The purpose of research is studying parameters of heart rhythm variability (VRH) at electric train’s machineries of underground (ETM). Materials and methods. Two groups of persons are surveyed: 30 - ETM (the basic group), 30 - workers of a nonproduction link (control group). All surveyed persons of the basic and control groups are men. Distribution of the main group of persons over the age: 10 person - till 29 years (27,61±0,63), 10 person - 30-39 years (36,22±1,14), 10 person - 40 years and older (51,17±1,88). Distribution of persons of control group on age is identical the basic. VRH it was estimated according to the uniform international standard [11]. Results. The analysis of histograms of distribution R-R of intervals in the evaluation of HRV IEP shown that in the majority surveyed histograms which distribution differs from normal are registered. 22% of the surveyed was asymmetric histogram which can testify to presence of several episodes of change of normal rhythmic which can be caused by emotional factors; at 41% ETM appeared excessive histograms. The last are characterized by very narrow basis and the pointed top and observed at stress [4]. In fact, during a labour shift organism ETM is influenced by psychological and emotional stressors and the nature of such interest excesses can be explained by the fact that the body can`t remain indifferent to the working conditions associated with significant sensory and emotional stress loading. In 37% of histograms distribution of intervals R-R is close to normal. In 100% ETM registered overactive sympathetic-adrenal system, which displays a certain emotional stress experienced by the ETM in the course of employment. Conclusions. Presence of a significant amount of histograms, which distribution differs from normal, testifies to negative reaction from side cardio-vascular system on psycho-emotional stress-factors the nature which accompany with activity at ETM. Keywords: electric train’s machineries of underground, variability of a rhythm

40-43

Authors: Yakymenko I., Mor O., Tsybulin O., Kolesnik Ya., Kyrylenko S., Sidorik E.

Цель работы: Изучение особенностей пользования мобильными телефонами студенческой молодежью и оценка субъективных симптомов у пользователей мобильной связью. Материалы и методы: Методом анонимного анкетирования проведена оценка особенностей использования мобильных телефонов и субъективных симптомов у пользователей мобильными телефонами – студентов Киевского региона Украины (n=600). Результаты. Выявлено, что 37,8% опрошенных ощущают физический дискомфорт, а 40% – боль в голове и/или ухе во время разговоров по мобильному телефону. При этом среди тех, кто пользовался мобильным телефоном не более одного года, головную боль во время разговоров ощущали 16,7% опрошенных, а среди тех, кто пользовался телефоном болем 10 лет – 50%. Выводы. Интенсивное использование мобильных телефонов студенческой молодежью вызывает чувство физического дискомфорта и болей в голове и/или ухе у значительного числа пользователей.

50-53

Authors: СУРМАШЕВА О.В., ШЕНЦОВА М.О., НІКОНОВА Н.О.

Development and use of the standardized reference samples are one of the actual elements of the system providing a quality of medicines. Sensitivity of microorganisms, included in the composition of the agent, to the antimicrobial preparations is an important feature at the choice of probiotic preparations and assessment of their efficiency. Objective. We studied a sensitivity of lactic acid bacteria strains – B. bifidum and E. faecium, selected for the creation of the standard reference samples of probiotic microorganisms, to the basic groups of modern antimicrobial agents. Results. The obtained results testify that the studied strains were sensitive respectively to 42% (B.bifidum) and the 43% (E.faecium) of the preparations actively used in clinical practice. Determination of the probiotic strains’ sensitivity to the antimicrobial preparations can be an additional epidemiologic marker for the selection of the antimicrobial treatment regimens, performance of the epidemiological monitoring of pathogenes.

58-61

Authors: Трахтенгерц Г.Я.

Objective: determining the hazard class of waste mining and processing complex of Ukraine: the tailings of iron ore and mud from alumina production and the study of the influence of location of these industrial wastes on the environment. Materials and methods: In the course of work we studied the tailings of iron ore, red mud from alumina production, their localization, soil, biota soil microorganisms. Were applied physics-chemical, sanitary-chemical, bibliographic and analytical research methods. Results: studies were identified particle size distribution and the chemical composition of the waste and on the content of toxic compounds (heavy metals) defined their hazard class. Revealed the presence of the area contaminated with heavy metals of the soil adjacent to the waste tanks territories. It is established that in many cases, this area is outside the sanitary protection zones of size 300 m It is proved that the flow in the soil tailings of iron ore and red mud from alumina production due to their toxic components reduces the enzymatic activity of the soil and negatively affects biological properties.

67-72

Authors: ОМЕЛЬЧУК С.Т., ШКАРАПУТА Л.М., ПЕЛЬО І.М., ТИЩЕНКО Л.О., СТЕПАНЕНКО В.І., ЦЕХМІСТЕР Я.В., ШЕВЧЕНКО Л.О., БЛАГАЯ А.В.

Mycosises Treatment (fungal infections), despite the great achievements in the study of the etiology and pathogenesis, remains today a challenge, which leads to the search for new effective and safe medicinal substances. Objective: assessment of the potential risk of side adverse effects on the basis of a combined teobon-dithiomycocide and gentamicin preparation for the treatment of fungal infections of the skin. Methods. Studied compositions were containing various concentrations of teobon-dithiomycocide and gentamicin sulfate (Gentamicin). Study of acute oral toxicity was conducted on Wistar rats of both sexes by injection to the food using a metal probe. Skin-resorptive effect was studied on rats of the same strain, guinea pigs and rabbits by applying a drug solution and ointment with 24 hours exposure. Irritant effect on skin and mucous membranes was studied in rabbits by application of 0.5 ml of the preparation to the clipped skin under semiocclusive dressing and instillation of 0.1 ml of the preparation in the conjunctival sac of the eye, followed by assessment of the results. Allergic properties have been studied in guinea pigs. Cumulative properties were studied in rats for 2 months by administration of the preparation to the stomach at 135 mg/kg dose daily, and by simultaneous application on the skin at 300 mg/kg dose. The dynamics of animals’ behavior, food consumption and water intake were evaluated, body weight was recorded, and the state of the cardiovascular system, external respiration, central nervous system, haematological, biochemical, histological and macroscopic (skin) parameters were studied. Results. It was established that combined antimycotic, containing teobon-dithiomycocide and gentamicin sulfate active substances, is low toxicity for white mice and rats when administered into the stomach, and when applied on the skin in mice, rats, rabbits and guinea pigs. At the studied concentrations (with a maximum of 5% teobon-dithiomycocide and 0.1% gentamicin sulfate) preparation does not irritate the skin and does not damage the structure of the skin and subcutaneous tissues, as shown by histological studies. Allergic properties of the drug have not been revealed. Cumulative properties when administered into the stomach and simultaneously applied to the skin were not expressed. Conclusions. In the experiment carried out taking into account the conditions of use in medical practice, it was found that the studied preparation in aqueous solutions and petrolatum-based ointments is non-irritating to the skin and mucous membranes, has no adverse effect on the cardiovascular, respiratory, central nervous systems, the morphological composition of the blood, liver and kidney function and structure.

78-79

Authors: ГУЩУК І.В.

Objective. We determined the ways for the development of the preventive directions in the sphere of public health in the period of sanitary-and-epidemiological service reform. Materials and Methods. Legislative and standard-methodological materials. Results. We analyzed the materials of the changes in the character of the activity of the sanitary-and-epidemiological service of Ukraine for the last years. Assessment of the recommendations for the increase of its efficiency for the near perspective has been given. We propose a creation of the alternative preventive service as a development of public health system and construction of the latter with the preservation of the material and technical basis and personnel potential.

4-7

Authors: СЕМАШКО П.В.

The objective. To establish the expediency of application of the combined equivalent Lden and Ldn sound levels as a criteria of hygienic assessment of the acoustic condition of residential areas. Materials and methods. The calculation of dependencies "level of traffic noise - an irritation" " was in accordance with WHO recommendations (Burden of disease from environmental noise. Quantification of healthy life years lost in Europe; Night noise guidelines for Europe). The calculation of the combined level was conducted in accordance with Directive 2002/49/EC of the European Parliament and of the Council of 25.06.2002 relating to the assessment and management of environmental noise. Results. Proved that violations of the existing permissible equivalent levels are possible with combined equivalent Lden, Ldn sound levels for hygienic assessment of the acoustic condition of residential areas. Is established that the combined equivalent Lden and Ldn sound levels is onenumerical criteria that do not consider the physiological human condition at different periods of CNS activity (work, rest, sleep). So they can`t be recommended for hygienic assessment of the acoustic condition of residential areas. Proved that the use of Lden and Ldn criteria to determine the percentage of public complaints (rated 'very irritates") to the traffic noise: underestimates the percentage of public complaints in comparison with the evaluation Lnight + Lday criteria (percentage of total complaints for night and daytime), underestimates the percentage of public complaints in comparison with the evaluation Lnight criteria (complaints at night), hides the distribution of complaints depending on the daytime, making it difficult to correct managerial decisions. Conclusions. Combined equivalent sound levels should not be used as criteria for evaluation of the sanitary condition of the acoustic condition of residential areas. The most appropriate criteria for hygienic sound assessment of residential areas are equivalent Lday and Lnight levels.

13-15

Authors: МАРАКУШИН Д.І.

The Objective: to analyze the content of serotonin and its precursor tryptophan in the brain of rats under the long-term influence of oxyethylized nonylphenols and their derivatives. Materials and methods. The experiments was carried out on the sexually mature rats-males of WAG population; rates orally received, before feeding, aqueous solutions of substances with 1/10 and 1/100 LD50 through a metal probe every day during 45 days. A level of serotonin and tryptophan in the brain of rats was determined by a chromatographic method on columns with cation-exchange resin. Results. We considered the regularities of the processes of adaptation and homeostasis under the influence on an organism of oxyethylized nonylphenols and their derivatives. Conclusions. It was established the influence of oxyethylized nonylphenols and their derivatives: sodium salts of carboxymethylates of oxyethylized isononylphenols on the processes of neuro-humoral regulation of rats, that is confirmed by disbalance of serotonergic neurotransmitter system of the brain. The decrease of serotonergic activity of the rats brain is proved in the conditions of the influence of the studied substances in a dose 1/10 DL50, and increase of serotonin synthesis (protectively-compensatory reaction of an organism) under the influence of substances in a dose 1/100 DL50.

21-24

Authors: ПАВЛЕНКО Т.О., АКСЬОНОВ М.В., ШАБУНІНА Н.Д., ФРИЗЮК М.А., ТАРАСЮК О.Є., КОВТОНЮК Н.Л., СЕМЕНЮК Н.Д., ФЕДОРЕНКО О.В., МИХАЙЛЕНКО О.В.

Management of waste in the oil and gas industry containing significant activity concentration of naturally occurring radioactive materials (NORM) is one of the major problems over the world. Activity residues, oil-slimes and used processing facilities can generate considerable radiation exposure to industry workers due to radioactivity materials entry in the industrial zone and environment. The aim of the article is to search the NORM activity concentration in the industrial residues of some oil and gas enterprises of Ukraine to establish appropriateness of the regulatory control over these enterprises. Gamma-ray spectrometry, mathematical and statistical methods were used. Results. It is made measurements of activity concentration of some natural radionuclides (226Ra, 232Th, 210Pb, 238U, 235U, 223Ra, 227Th, 40K) in activity residues of some Ukrainian oil and gas enterprises. Basic statistical indicators of NORM activity concentration levels in residues of each enterprise were calculated. It is established that IAEA levels for clearance of material regarding activity concentration of radionuclides of natural origin 1 Bq•g-1 (1000 Bq•kg-1) aren’t exceeded in solid residue in respect of all mentioned natural radionuclides in two enterprises. Whereas scarcity of researched material it is recommended the additional residues measurements of industrial activity concerning these enterprises. Exceeding the IAEA levels for clearance in respect of four natural radionuclides (210Pb, 226Ra, 232Th, 238U) in waste of the oil and gas industry and solid residue are detected at two enterprises. It is recommended regulatory control over these enterprises.

31-34

Authors: ГАРКУША С.Л., КОРЗУН В.Н.

The objective. Development of methods of primary atherosclerotic cardiovascular disease prevention among a population. Materials and methods. Estimation of blood lipid spectrum, specifying the type of dyslipidemia and the degree of overweight with a body mass index, definition of the general and individual cardiovascular risk in the offices of the correction of overweight and treatment of various forms of obesity. Results. Outcome of primary and secondary prevention of atherosclerotic vascular disease among a population is improved by complex examination of patients with the purpose of timely revealing of the metabolic syndrome (abdominal obesity, hypertension, dyslipidemia, hyperuricemia, insulin resistance, diabetes mellitus) and destination multimodality therapy, monthly monitoring in achieving the target levels of lipid parameters, arterial tension and normal body weight. Conclusions: Implementation of primary prophylaxis methods that include the identification of patients with metabolic syndrome and assessment of cardiovascular diseases developing risk, leads to reduce cardiovascular morbidity and mortality from infarction and cerebral thrombosis.

38-44

Authors: ПОЛЬКА Н.С., СЕРГЕТА Д.П.

Objective. To study the scientific method of complex numerical score risk of adverse changes in the leading indicators of physical development and morphofunctional condition of the newborns. Materials and methods. Assessment of physical development and functional state of newborns and their mothers was carried out using conventional anthropometric techniques. Leading medical and social features of mothers life and lifestyle were determined through questionnaires and interviews, to determine the personality traits of mothers was used the psychodiagnostic methods. Results and discussion. The method of complex numerical score risk of adverse changes in the leading indicators of physical development and morphofunctional condition of the newborns is scientifically grounded during the research. It is an adequate to modern requirements, easy to use and informative tool, from prognostic position, to determine the prerequisites for the formation of alleged misstatements in the process of becoming morphofunctional state of the newborn girls and boys. The main stages of the practical interpretation of the methods are: determination on the basis of the application of sociological and psychodiagnostic methods of expressing the degree of medical and social characteristics, features of daily activities and mothers personal characteristics (stage 1); evaluation of the results obtained in points according to a specially designed scoring scales (stage 2); calculation of integrated indicators for assessing the risk of adverse changes in the leading indicators of physical development and morphofunctional condition of the newborns according to the medical and social, regime-significant and personal prognostic determinants (stage 3); estimation of the integral index, calculated on the basis of quantitative criteria for their content evaluation (stage 4); determining individualized, according to the direction and content of each of the determinants, strategies prophylactic activities and psychohygienic correction of deviations (stage 5).

50-52

Authors: ЗАВАДА М.І.

Objective to determine the criteria of unsatisfactory adaptation prediction to studying of children enrolled in the first grade of secondary boarding schools. Materials and methods. Adaptation of 235 first graders boarding schools was evaluated for the annual dynamics of physical development, cardiovascular and psycho-physiological development. Results. Growth inhibition in children in the end of I-st quarter, I-st semester and year, absence of changes or diminishing in these terms of body weight, the quality index of blood circulation as a reaction on loading, speeds of visual information processing in more than half of children specify the delay of adaptation in the expected terms of its offensive. These processes are peculiar to the children who on entering school were characterized by low rates of reaction (≤0,01 conditional units), by readiness for training (test-Kern Jirásek ≥9 balls), by speed of processing of visual information (≤0,2 bit/s), by the body mass index ≤15 or ≥16 or in combination of two or more unsatisfactory ratings on these indicators. This complex is offered as criteria for the formation of groups at risk of unsatisfactory adaptation for entering children to boarding schools.

62-37

Authors: Крупка Н.О., Лотоцька-Дудик У.Б.

The aim of the work was to analyse regularity of formation, accumulation, methods of treatment and utilization of waste in Lviv region with the subsequent formation of the ways to increase environmental and economic efficiency of their reuse. Materials and methods. Materials of the Program of hard domestic waste treatment in the Lviv region, State committee of statistics of Ukraine, Main Statistical Office and Main Department of Environmental Protection in the Lviv region, the results of own research were used for an analysis and evaluation of Waste Issues in the Lviv region. Results. In the last few years the tendency to the increase of waste accumulation is observed. It causes the increase of amount of unauthorized dumps and grounds. The ways of waste and the main indicators of treatment were analysed. Public opinion about the implementation of separate collection of municipal solid waste was studied. The main problems of waste management and ways to solve them were indicated and outlined. Conclusions. For solving the problems of education, treatment and disposal in the Lviv region is necessary to form a regional co-ordinating center for waste management, introduce the main disposal methods involving new technologies and investments.

71-74

Authors: КАРЛОВА О.О.

The Objective. The study of the features of laboratory and instrumental changes in workers exposed by lead, the conducting the prognostic evaluation of the studied parameters. Materials and Methods. The 203 patients were examined on the basis of MCU number 18 of Ministry of Health of Ukraine in Kiev. All patients were divided into 3 groups at depending on the level of blood lead. The І group included the patients with the level of blood lead of 2,12 ± 0,013 micromole/l, in ІІ group — 1,92 ± 0,013 micromole/l, in ІІІ group — with a minimum level of blood lead is 1,72 ± 0,028 micromole/l. Age of patients was ranged from 35 to 47 years. The average age of patients for the І group (51 people) was 43,5 ± 1,3 years, the ІІ group (46 people) — 39,9 ± 1,8 years, the ІІІ (49 people) — 40,3 ± 1.4 years. The control group included 57 healthy persons, whose average age was 44,7 ± 1,5 years. Only male were examined. Results. The paper presents data on the characteristics of ultrasound changes of the hepatobiliary system with lead exposure. The results of the ultrasonic examination of the liver depending on the level of lead in blood are shown. Investigated basic biochemical blood indices that reflect the functional state of the hepatobiliary system (bilirubin, ALT, AST, Ritis ratio, total cholesterol, blood glucose). To evaluate the diagnostic efficiency indicators we defined diagnostic (prognostic) significance for the individual levels. Performed calculation of the following parameters: sensitivity, specificity, and diagnostic accuracy (efficiency), the relative risks. For all diagnostic characteristics determined the confidence interval and checked the statistical significance at not less than 95%. Used the method of ROC-analysis to determine the threshold levels of the indicators used as markers of the severity of changes in workers exposed lead. Conclusions: steatosis and diffuse changes in the liver are the most common in the structure of sonographic pathology the frequency of which are increases with the blood lead levels. In group I patients had the maximum values of transaminases ALT and AST in the backdrop of reducing de Ritis index, which indicates the genesis of toxic liver damage upon exposure. CWR value of the right lobe more than 148 mm is the threshold level for the index ultrasound examination of the liver. The excess of these levels is associated with high blood levels of lead and has a statistically significant predictive efficiency (p<0.05).

4-9

Authors: Винарська О., Черниченко О., Спаська Ю.С., Григоренко Л., Баленко Н.В., Соверткова Л.С.

Objective. To substantiate criterial - significant immunologic effects of endogenous nitrosamines and their precursors. Materials and methods: Nitrosamines precursors were administered to random-bred white rats: tetracyclin (4 mg/rat) in food, sodium nitrite (4, 10, 20 mg/rat) in drinking water. Methods: gas-chromatographic (determination of N-nitrosodiethylamine (NDEA) and N-nitrosodimethylamine (NDMA), in the animals organs; hematological, immunological (white blood cell count, formed elemnts count, T- and B-lymphocytes, and natural killer cells,immediate and delayed hypersensitivity types, phagocytosis, the level of circulating immune complexes); the pathomorphological, statistical. Results. Dependencies of immunological effects "a dose of precursors - he content of endogenous nitrosamines" in the organs of experimental animals are established. Revealed a dose-hour regularities of manifestation of immunotoxic effect of endogenous carcinogens and their precursors. The carcinogenic danger of the endogenously synthesized nitrosamines was proved which was appeared by development of tumors in depend on a dose of precursors. The highest dose of sodium nitrite (20 mg/anim) in a combination with tetracycline (4 mg/anim) leads to the suppression of T- and B-links of immune system, decreasing the number of natural killer cells, to development of immunologic reactions of I, III, IV types at an early stage of formation of endogenous nitrosamines and induces tumors in 72,7% of animals. By reducing dose to 10 mg/anim. all of the immunological effects occur only after 6 months of exposure. Decreasing of tumors development to 13,3% of cases was also established. Results of researches allowed to subtantiate of criterial the list significant immunological parameters.

14-48

Authors: Гуліч М.П., Харченко О.О., Ємченко Н.Л., Єрмоленко В.П., Моісеєнко

The objective of the work was to study the composition, properties, and chemical purity of magnesium nanocitrate; to obtain the toxicological and biological characteristics. Research objects. Solutions of magnesium citrate, produced by aqua nanotechnology, and inorganic compound - sulphate of magnesium. Materials and methods: a method for chelatometric titration with the acidic chromium dark blue as an indicator was taken for the determination of magnesium. Citrate-ion was determined by the HELC method. Determination of magnesium in biomaterial was performed by the atomic-absorption method. Assessment of chemical purity of these citrates performed of spectral analysis. Results. Сhemism of citrate, produced by nanotechnology, complied with the same of typical magnesium citrate. Spectral analysis of the compounds, extracted from the solutions, for the determination of the content of Si, Al, Cu, Fe, Ca, Cr, Ti, Mn, and Ni in them and also a voltamperemetric determination of Pb and Cd in them showed that total content of these nanocitrates of admixtures varied from 0.03 up to 0.13%, that allowed to qualify them as “ultra pure”. Comparative study of magnesium nanocitrate and its sulphate had been performed on the white mice at the intragastric administration of these substances, containing magnesium in a dose corresponding their daily need, during 14 days showed that its level was authentically higher (by 10-40%) practically in all samples of organs and tissues (especially of bone tissue) of the experimental animals than in the samples obtained from the mice which were administrated MgSO 4 solution. That testified about higher assimilability of this metal from its nanocitrate. Thus we have determined that magnesium nanocitrate close to the typical citrates by stoichiometry doesn’t differ from them by chemism and reaction ability. They are not toxic, chemical cleaner in comparison with the commercial magnesium citrate, differ of larger biological availability and therefore can be used for the enrichment of the foodstuffs with magnesium.

24-29

Authors: Кононко В., Щербицька О.В., Кліпов В.Д., Сергєєв В.П., Уварова В.

The objective: generalization of knowledge about environmental issues and the search for effective and harmless nanomaterials and nanotechnologies to solve environmental problems. Мaterials. Characteristics of activated nanostructured fibrous carbon material (AUVM “Dniepr”-MN) designed at Frantsevich Institute for Problems of Materials Science of NAS of Ukraine on the basis of rayon fiber are presented. Presence of nanostructures in the form of graphite nanopackages, nanobulbs, nanofibers, and nanotubes in it has been shown. Methods for investigation. Activated and modified fibrous carbon materials in the form of different textile structures such as knit and soot were used as samples to be studied. Porous structure of the samples was estimated via absorption of benzene vapors. The specific surface area was determined using the BET method. The concentration of phenol was measured by the colorimetric method. Sorption of metals was conducted in a static mode. The metal concentration was determined using an atom-adsorption spectrometer AAS3 and standard techniques for control of drinking water quality. The content of active chlorine was established by an iodine-metrical analysis. A special technique was used for estimation of the adsorption capacity of adsorbents towards iodine and methyl iodine vapors. Results. The peculiarities of AUVM structure formation have been studied depending on the conditions of fabrication, activation and modification of carbon fibers. The porous structure of AUVM as a sorbent and its sorption characteristics as well as the kinetics of adsorption of harmful substances namely phenol, heavy and toxic metals, chlorine and its compounds in the course of water purification were investigated depending on their concentration in solutions. The universal sorption activity of AUVM towards a number of environment pollutants has been demonstrated, which evidences to its possible successful application both under extraordinary conditions and in everyday life. The developed AUVM was shown to have significant advantages over traditionally used in industry activated carbon of the trades SKT, “Buosofit” and BAU. The possibility of using AUVM at nuclear plants as a filter filler in air purification systems and special systems for air purification from vapors of iodine and iodine methyl was demonstrated.

34-36

Authors: Долінчук Л.В.

The Objective. Define the role of TІMP-2 gene polymorphism ( rs9900972 ) in the development of a genetic predisposition to COPD in order to improve early detection and primary prevention of disease. Materials and methods. The genotypes of the TIMP-2 gene polymorphisms ( rs9900972 ) was determined in 72 patients with COPD and 79 healthy controls. Real-time polymerase chain reaction (PCR) was used for genotyping of polymorphisms in TIMP-2 gene. Results and discussion. The study determined frequency distribution of alleles and genotypes for gene TІMP-2. Determined the amount of OR and identified an association between genotypes TIMP-2*GA (OR = 1,77; 95% CI: 0,83-3,77) and TIMP-2*AA (OR = 3,29; 95% CI: 0, 35-30.94) at high risk of developing COPD in the study group. We also have found that genotype TIMP-2*GG cause the resistance to the development of COPD (OR = 0,51; 95% CI: 0,24-1,06). Conclusions. The protective role of the dominant allele TIMP-2*G and genotype TIMP-2*GG (p < 0,05) in miners working in conditions of high concentrations of coal dust was founded. Shown, that the minor genotype TIMP-2*AA can be associated with the risk of COPD in the study group.

42-45

Authors: Синетар Е.О., Покас О.В., Авдєєва Л.В.

To determine the action of selenium on nanoaquahelate biofilm formation of E. faecalis and C. albicans in association on the silicone catheter. Methods. The study was performed using bacteriological and electron microscopic techniques. Results. We found C. albicans cells association with subsequent groupings (agglomerates) and a smaller number of attached cells of E. faecalis on the outer and inner surfaces of the unprocessed nanoselenium catheter fragment. At the same time, the internal and external surfaces of the catheter fragments treated nanoselenium at a concentration of 0.01 mg / ml was observed lower number of attached cells of E. faecalis and C. albicans compared to controls. Conclusions. The drug nanoaquahelate selenium concentration of 0.01 mg / ml reduces the number of attached cells of the test strains, affecting the change in morphological traits (shrivelling) cells of C. albicans which may indicate the effect of selenium on the cytoderm of the test strain. Concentration of nanoselenim 0.0025 mg / ml led to the formation of biofilm in the test strains for 24 hours, and the drug is not recommended to be used in low concentrations.

49-54

Authors: Капустинська О.А.

Characterization of the dynamics and structure of the circulatory system (CS) pathology of evacuated taking into account the age at the time of the accident, sex, and time at risk. The observation period 1988-2010. Research Methods. As the source of information served the data of the State register of persons affected by the Chоrnobyl disaster. In order to settle the matter the cohorts had been formed from the evacuated people who was registered. Results. The results of the epidemiological researches testify that the post-repair period was marked by the increasing the number of blood-vascular system disease. The main increasing was observed after 12-22 years repair. The substantial changes took place in the structure of diseases of blood-vascular system. On the first stage (5 years) hypertension took the first place, the next ischemic heart disease followed and cerebrovascular disease took the third place. On the stage of 17-22 years after repair the picture has changed - ischemic heart disease took the first place and hypertension occupied the second one. Cerebrovascular disease kept the third place. With the extension of the observation period the level of cerebrovascular disease increased from 6,5 percent in 1988-1992 to 15,8 percent in 2009-2010. The increasing of the diseases level found out in both men and women, but men’s level of disease exceeded considerably. Men’s indexes reached the maximum in 2003-2008. Next years it was noticed substantial lowering of index (authentic lowering in 2009-2010 in comparison 1988-1992 and 2003-2008). Women’s rise of morbidity has marked to 2003 with maximum in 1998-2002 and then the indexes went down. As regards to changes in the dynamics of incidence on separate nosological entities, the significant difference observed in the level of hypertension, ischemic heart disease, cerebrovascular disease. Severe complications such as stroke, cerebral infarction, myocardial infarction is more common in men in the later periods of observation. Through 22-24 years it is established a clear downward trend in the incidence of a test contingent, which is associated with the implementation of these diseases, and with a significant increase of mortality among certain categories of victims. Conclusion. Nowadays the analyses of the CS morbidity level depending on the dose of the irradiation observed thyroid gland sickness, the external radioactive irradiation and the separate factors of the non-irradiation characters (Clinical-epidemiological observation data) is being carried out. It will help to establish the possible dependence of the growing blood-vascular system morbidity in adult evacuated population at the post-repair after the effects of radioactive and separate non-radioactive factors.

61-62

Authors: Трахтенберг М.

The authors find it necessary to focus particular attention on present-day questions and problems in settlements’ planning health and safety due to the introduction of innovative processes in science, reform of central authorities, the reorganization of the State Sanitary and Epidemiological Service of Ukraine. The legislative and regulatory framework on settlements’ planning hygiene was analyzed and specific measures for the legislative regulation of problematic issues were suggested.

75-79

Authors: Савіна Р.В., Савицька О., Булгаков В.В.

Objective. Comparative analysis of the main indicators of scientific activity SI „IGME” for 2010-2013 years. Materials and methods. Volume documentary array of scientific output of the Institute amounted to more than 1,400 units. We used the analytical, statistical and mathematical methods. Results. Information materials of research institute were collected and processed. Was marked the positive dynamics of presenting the achievements of Ukrainian hygienists in international scientific journals. Results of the scientific researches are reflected in more than 1 116 publications, including 112 foreign. The institute employees are participated in more than 600 scientific forums, actively interacting with the institutions and organizations of various ministries and departments: prepared 94 regulations, 26 patents, 63 newsletters. Found that the research results of SI "IGME NAMSU" scientific units is widely implemented in the practical work of the various ministries and departments of Ukraine.

4-10

Authors: Тимченко О., Процюк О.В., Омельченко Е.М., Линчак О.В., Поканєвич Т.М., Микитенко Д.О., Качко Г.О., Кабанець Т.М.

Demographic crisis and environmental degradation changed the live conditions of the inhabitants; that may influence biological characteristics of human population and its adaptation possibilities. The objective to estimate the Ukrainian population adaptation level by its reproductive potential, selection size, weight of newborns and congenital malformations frequency among them, marriage migrations outcomes. Materials and Methods. The data of State Statistics Committee of Ukraine and Ministry of Health of Ukraine within 2002-2012 were analyzed. Was estimated the specific gravity of optimal reproductive women age and 0-14 years old children. Was estimated the relative risk of low birth weight, perinatal death and infertility in regions relative to the national average except those for which the relative risk was calculated. Newborns weight data were received from maternity hospitals documentation over generation in Cherkasy, Zhytomyr, Kyiv regions. Marriage distance was estimated in the cases of infertility, spontaneous abortions, congenital malformations and healthy children birth in Kyiv region. Were used MedCalc and Microsoft®Excel 97. Results. The reproductive potential of Ukraine is decreasing. The values of the selection: reproductive losses - 41,92±0,13‰, mortality in before -reproductive age is (0,96±0,01)‰, including mortality from congenital malformations (0,19±0,01)‰. XXI сentury children have reduced weight, which may mean the increasing of mutations and recombination (with environmental influence). Prevalence of CDF among born alive, still-borne and eliminated fetuses was (26,83±0,08)‰ in 2002-2011. The hypothesis about negative influence of high heterozygote level on adaptation to live conditions was proved. According to investigation results the population of Ukraine is disadapted. As accommodation is a relevant size, environmental improvement may cause positive changes of adaptation potential.

15-18

Authors: Яловенко О., Раєцька О.В., Голіченков О.М., Бабій В.Ф., Кондратенко О., Пімушина М.В.

It was the purpose of our work to define safe surfactants and to compare the degree of their cytotoxic action by an alternative in vitro method with the use of cellular test-object of suspension culture of spermatozoa of bull. Materials and methods of researches. 11 surfactants of different groups are explored in work: anionic, amphoteric, nonionic end сationic. Estimation of safety (cytotoxicity) was conducted by the in vitro assay on the culture of spermatozoa of bull at the use of analyzer of toxic of AТ-05. Results of researches. In researches of in vitro is set, that from 10 surfactants only one (polyquaternium 7) are safe (behaves to a 4 class of danger and does not cause skinning-irritating action), on the degree of expressed of cytotoxic action most dangerous surfactants - coco-betain, sodium lauryl sulfate, sodium layreth sulfate, cocamidopropylbetaine; the normative breeding of surfactants for conducting of comparative researches of their cytotoxic action is certain.

24-28

Authors: Калиниченко О., Скиба О.О.

The objective of this research was to determine the influence of conditions and organization of training process on the functional state of the children’s organism engaged in different kinds of sports. Study methods. Hygienic (hygienic examination and monitoring), mathematical methods. Results. We determined the leading sanitary-hygienic factors of the training process and a degree of their influence on the parameters of functional state of children’s organism engaged in different kinds of sports in the system of the sport schools for children and youth. We determined the indices of air-heat regime of training premises affected greatly the functional state of young sportsmen organism. The degree of the influence of conditions and organization of training process on the indices of the functional state of the organism of children depends on the specificity of the kind of sports. Sanitary-hygienic factors affect mostly on the muscle strength among the representatives of single combat and for children engaged in complicated coordinating kind of sports they affect greatly the indices of spinal mobility. The greatest influence of the conditions of training process on the indices of physical efficiency is typical for the representatives of cyclic kinds of sports while for the young sportsmen engaged in sport games - on the indices of muscle strength.

34-38

Authors: Кондратюк В.А., Федорів О., Лотоцька О.В.

The objective of the research was to study and compare a combined effect of lead acetate nanoparticles and lead macroparticles in the composition of lead acetate in water on a background of potassium and sodium stearates on the processes of lipid peroxide oxidation in liver and kidneys of white rats. Materials and methods. The experiments have been performed with 5 groups of white female rats of 150-200 grams, 7 animals in each group. The 1- st group was a control and took potable dechlorinated water from the city water supply system. The rats of the 2- nd and 4- th groups took the same water but with the potassium stearate, and animals of the 3- rd and 5- th groups took water with the sodium stearate (1/250 HD 50). After 40 day usage of mentioned types of water the animals of the 2- nd and the 3- rd groups have received lead acetate preparations as nanoparticles per orally, the animals of the 4- th and 5- th groups have received lead acetate preparation in a dose of 7 mg/kg by the content of lead acetate. In 3 days the animals have been taken out from the experiment by means of phlebotomy under thiopental anesthesia according to the rules of bioethics. Results. We determined a statistically valid increase of the activity of dien conjugates and malon dialdehyde in the tissues of liver and kidneys of the experimental animals in the animals with per orally administrated lead acetate on a background of the use of water with potassium and sodium stearates in comparison with the animals administrated with lead nanoparticles. At the use of water with a complex of potassium stearate, lead nanoparticles and lead acetate we observed a more expressed toxic effect on the process of peroxide oxidation of lipids in animals’ organisms then on a background of the use of water with sodium stearate.

43-46

Authors: Бузинний М.Г., Михайлова Л.Л., Романченко М.О., Чирков В.С., Сахно В.

The objective. To study 14C content variation in the atmosphere by measuring the specific 14C activity in the annual growth of trees for background conditions and under the effect of the nuclear-energy complex site as well as the comparison of the 14C spatial distribution around the object. Materials and methods. 14C content measurements in the annual growth (tree rings) of pine wood for 14C variation studies in the air for the time interval since the end of 40- th of the last century - the period when there have been the greatest fluctuations in radiocarbon. Research objects - annual pine wood growth. Trees for the study of background oscillations were selected in Chernigiv region and for the influence of anthropogenic emissions 14C study were selected trees near the source of emissions 14C, studied earlier. In the second case, we examined the wood material of tree that grew at a distance of about 5 km from the site, and compared the results with those obtained previously for a tree that grew at a distance of about 10 km. Measurements were carried out at 14C using a traditional method based on a liquid-scintillation counting. For this study used the charred wood annual increment, of which have been previously extracted resin material in a Soxhlet apparatus. Samples of benzene were measured using a Quantulus 1220 TM LS spectrometer made by Perkin Elmer Inc. Results. Technology used in the preparation of baseline studies of samples allows to obtain results similar to the direct measurements of 14C in the air. Studies of samples taken in the area of ​​ nuclear facility influence showed the results concerted with previous studies of the impact of the object. Comparing the relevant research showed that the samples of the annual increment in some years have significant differences, confirmed by repeated studies. The differences can be explained by inappropriate spatial distribution at close distances from the object. Overall consistency of the data sets suggests that the earlier estimate of annual emissions and 14C emissions for the time of work does not require the re-evaluation.

51-55

Authors: Янко Н.В., Станкевич В.В., Коваль Н.М.

Data on the retrospective analysis of the results of the study of water quality of the lake Svitiaz, Shatskу National Park by the most informative indices of the eutrophy level of the basin (general phosphorus, biochemical use of oxygen, suspended substances) for 1985-2010 are presented in the article. Objective of the work. Determination of the effect of the recreational loads on the eutrophy processes of the lake Svitiaz. Objects and methods for study. In the samples from the lake Svitiaz, selected in 1985-2010, a content of general phosphorus, suspended substances, and biochemical use of oxygen (BUO 5) were determined with the help of generally accepted methods. Study results. It was determined that an excessive recreational load on the water resources of the lake Svitiaz without sufficiently developed infrastructure decreased an anthropogenic load on the basin. It leads to an increase of trophicity level, and for the future - to the high trophic and hypertrophic levels. Strengthening of the process of water flowering with a dominance of Cyanophyceae is expected. It will lead to the impossibility of the use of the lake Svitiaz, the important object of water recreation for all North-West regions of the country, for the recreational purpose. The obtained study results demonstrate a necessity of an urgent development of the measures for a decrease of the growth of water trophicity in the lake Svitiaz.

60-64

Authors: Брич О., Синетар Е.О., Лоскутова М.М., Гарницька О.Г., Скуратова О.Г.

Objective: to test the viability, taxonomic position of museum strains genera Proteus and Morganella during long-term storage - for 25-48 years, to examine their morphological, cultural, biochemical properties and sensitivity to antibiotics. Materials and methods: studies were perfomed using cultural, microscopic, bacteriological, biochemical and statistical methods. Results: found that among the studied strains of lyophilized P.vulgaris, P.mirabilis and M.morganii after 25-48 years of storage 84% was viable. Biochemical identification of museums strains helped to clarify the taxonomic position of the three isolates. A retrospective analysis of data sensitivity viable strains to antibiotics was made. All srains P.vulgaris and M.morganii were resistant to ampicillin, cefalotin, cefuroxim, cefuroxim acsetyl. All investigated strains were sensitive to aminoglycosides, quinolones, meropenem and trimethoprim/sulfamethoxazole.

70-75

Authors: Корзун В.Н., Гозак С.В., Парац А.Н., Иванов В., Белозуб А.

The objective. Study the effect of vitamin-mineral complex „Vitacor Junior” for children health, their physical development and school performance. Materials and methods. Children of the main group (n = 69) during 7 months daily received one tablet of vitamin-mineral complex, children of the control group (n = 34) - only school meals. Examination of children was carried out before and after supplements. The study was conducted on the basis of the school in Zhytomyr region. Results. After 7 months, children of the main group have improved functional state of the muscular and cardiac-respiratory system, decreased the incidence and likelihood of acute forms of the disease. Average level of acute morbidity is decreased by 25,5% (from 1,83 ± 0,13 to 1,37 ± 0,13 incidents in year, t = 2,44; p<0,05) compared to the similar index to reception of the complex; the reduction of probability of 2-4 acute diseases in the year is decreased by 2.83 times (RR = 2,83; 95% CI 1,46-5,49; EF = 64,7%). Functional status of cardio-respiratory system by index Skibinskу is improved by 28,2% (from 5,82 ± 0,42 to 7,46 ± 0,57, t = 2,29; p<0,05), diastolic blood pressure and heart rate is decreased (t = -3,64; p<0,001 and t = -2,43; p<0,05, correspondently). Functional status of the muscular system is improved by indication of the strength of muscles of the hand by 16.2% (t = 2,15; p<0,05) and by evaluation of the power index (χ 2 = 16,33; p<0,01) It is established that the proportion of children with low adaptation-reserve opportunities of an organism in the control group tends to increase (y = 18,2, x - 0,1), in the main group - to decrease (y = -7,2, x + 49,2). Conclusions of the study. A vitamin-mineral complexes contributed to the improvement of health status of children, which can be recommended for use in children nutrition.