Antibiotic Sensitivity Pattern of Salmonella Typhi in a Stand Alone Lab in Central Madhya Pradesh
Authors: Ranjana Hawaldar, Sadhna Sodani, Hemlata Bhilware
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Introduction: Typhoid fever or enteric fever, is an endemic disease in many developing countries specially India and the Indian Subcontinent. In the past decade, resistance of Salmonella to multiple drugs is on the rise specially resistant to ampicillin, chloramphenicol and cotrimoxazole. This resistant pattern has resulted in use of Fluoroquinolones and third generation Cephalosporins as first line drugs.)The present study was under taken to study the antibiotic susceptibility pattern of S.typhi isolated from blood cultures in our microbiology laboratory.
Materials and Methods: 766 patients suspected of having typhoid fever of different age groups and sex visiting our Microbiology department for blood culture were included in the study from January 2015 to December 2015. Gram negative non lactose fermenting colonies were subjected to identification and antibiotic susceptibility testing in Vitek II (Biomerieux) according to CLSI guidelines.
Results: Out of 766 blood cultures, 32 cases of S.typhi were isolated. 561 cultures were sterile and 73 showed growth of other bacteria. Colistin, Cefuroxime, Imipenem, Meropenem showed 100% sensitivity. Piperacillin/Tazobactam, Tigecyline. Cefoperazone/Sulbactam were sensitive in 93.75% isolates. Amoxicillin/Clavulanic Acid (90.62%), Ceftazidime(87.5%), Trimethoprim/Sulfamethoxazole (87.5%), Ticarcillin(84.37%), Ampicillin (81.25%),Cefepime(78.12%), Cefalotin(75%), Amoxycillin(65.62%), Cefixime(59.37%). Cefoxitin(43.75%), Amikacin(31.25%), Gentamicin(6.75%), Ciprofloxacin was found to be resistant in all 32 cases(100%).
Conclusion: There has been a reemergence in the sensitivity of Salmonella Typhi to Ampicillin, Ceftazidime, Cefuroxime Ceftriaxone, Cefoperazone/Sulbactum Cefipime and Amoxyclav and 100% resistance to Ciprofloxaxin and to Amikacin & Gentamycin. Cefuroxime, Ceftriaxone, Cefoperazone/Sulbactum seem to be good therapeutic options is our setting atpresent.