Visnyk of Dnipropetrovsk University. Biology, medicine

9-14

Authors: V. S. Bil’chuk, А. S. Rossikhina-Galycha

Influence of nickel ions on growth reaction in maize seedlings of Orjitza 237MV hybrid and the content of low-molecular antioxidants in assimilation organs are investigated. The dependence of morphometric parameters of seedling roots and sprouts on the concentration of nickel ions is established. Considerable depression of growth and development of vegetative organs at a juvenile period occurs under long-term action of the metal in concentrations of 0.05–1.00 mM/l. The changes of reduced glutathione and ascorbic acid content under nickel ions influence are found in the leaves photosynthesizing tissue of the maize seedlings. It leads to a variation of the reducing activity in the tissues. The given fact can testify to an oxidizing stress under nickel ions effect and to participation of the low-molecular antioxidants in the plants adaptation to xenobiotics influence. Period of renewal of the glutathione and ascorbic acid metabolism in experimental samples was determined by the concentration of the nickel nitrate.

20-25

Authors: O. S. Voronkova, E. A. Sirokvasha, T. N. Polishko, A. I. Vinnikov

The data on bacteriophage preparations, their features and benefits in comparison with other therapeutic agents are shown. The matters of phages production history to the present time are described. The data of phages application in clinical practice for the treatment, diagnostics, infections monitoring, sanitation of facilities and equipment, and conducting scientific research are shown. The benefits of phages drug are shown: affect only on specific sensitive bacteria, do not compromise the immunity, as opposed to antibiotics do not cause bacterial resistance, penetrate well into the humans and animals tissues, do not suppress the growth of normal flora and could be combined with any medication.

32-36

Authors: I. V. Zhernosekova

Physiological activities of Streptomyces recifensis var. lyticus P3X, culture liquid biopreparations and commercial preparations of Heteroauxin and Emistim were studed in the haricot bean (Phaseolus vulgaris). Stimulation of plants’ morpho-biochemical indices by 20.2–176.3 % was shown after the application of streptomyces preparations. Commercial preparations had no stimulation effect of the plants, except the Emistim effect on germinating power. Heteroauxin and Emistim oppressed some biochemical indices of plants by 22.6–55.6 %.

42-47

Authors: O. Р. Korzhenevs’ka, O. В. Severynovs’ka

With the help of daily monitoring of blood pressure we assessed the state of the cardiovascular system of foot-platemen of the Prydniprovsk railway Pjatykhatky Depot. Foot-platemen having the I stage of arterial hypertension demonstrated the 11–17% increase of arterial pressure during daily work. It is related to inefficient adaptation to the physical and psychoemotional overloads. With aggravation of the disease the change of arterial pressure during the work, especially night work, ismisaligned and characterised by considerable structural abnormalities of the left ventricle myocardium.

54-59

Authors: M. P. Motsnyj, N. P. Botsva, О. V. Elina, Y. A. Ulanova

Dynamics of maize leaves biopotentials evoked by X-rays stimuli is analysed. Qualitative similarity in dynamics of plant’s bioelectrical response is determined. Hyperpolarization levels are quantitatively estimated for every series of the experiment. Dependence of the summarized hyperpolarization levels on photostimulus wave length is detected. The fact is ascertained that mean amplitude of hyperpolarization potentials reduces from 57.7 to 27.7 mV when the wave length decreases. Probable mechanisms of the initiation of photostimulated bioelectrical response are analysed.

71-80

Authors: I. T. Rusev, V. N. Zakusilo

Analysis of mass outbreak of tularaemia in humans of Kosovo after the mass bombardments of the territory in 1999 suggests the possible anthropogenic nature of its origin. The emergence of mass epizooty among wild mammals and mass outbreaks of the disease among people of that dangerous infection may be possible in the case of activation of natural foci, or as a result of the infection import. Natural foci of tularaemia in Kosovo were not registered earlier. Consequently, outbreaks and epizootics of tularaemia may have the anthropogenic nature.

89-95

Authors: I. G. Sushchenko, О. М. Leshchuk, I. V. Dregval, А. I. Rudenko

Basic descriptions of the rats liver microvasculature and its prognostic significance under development of pancreatitis of different severity were determined by arheography method. Increased nitric monoxide content in the rats entails even more pronounced reduction of a tone of the liver’s great vessels. The vessels of medium and small diameter are less influenced and the reductionwasshort-term. Using the rheohepatogramme indices makes possible to determine the disturbance of liver’shaemodynamicsunderthe hepatopathy development.

10-17

Authors: V. G. Ginzburg, T. N. Polichko, P. M. Polushkin, D. V. Galchenko

Taking into account modern achievements in medicine, psychology and sociology, the attempt at complex research of possible inclinations for psychostimulant, toxic agent and drug abuse among youths and students was made with the subsequent determination of the possible alternates of primary prevention. It is analysed the basic and additional risk factors promoting smoking, drinking, psychostimulant abuse, toxicomania and narcomania among young people. The dynamics of possible influences of medical, psychological and social factors is studied. The attempt of short-term prognostication and ranking was made.

24-28

Authors: K. V. Derkach

The ability of the maize inbred lines of the Lancaster germ plasm to maintain morphogenic potential of the callus under long cultivation has been estimated. The capability to long-term morphogenic callusogenesis is genotypically determined and longer in the Mo17/Oh43 subplasm line. The line of Oh43 subplasm may be the base for the long-continued cultivation of morphogenic calluses. Regenerated plants from the long-continued cultivated calluses were obtained. Promising inbreds for usage in biotechnology and breeding programs were identified.

38-43

Authors: T. Y. Lykholat, S. V. Antoniuk, V. І. Probachay

Characteristics of the receptor response of tumour growth factors in women with breast cancer varying according to the women’s age were investigated. The differences between the receptor profiles of the primary tumour and of the metastatic breast tissue have been found. The tumours characterised by a combination of antigen Ki-67 expression and hyperexpression of proliferative growth proto-oncogene Her-2/Neu are particularly aggressive. In case of such combination the hyperresistance to treatment is determined.

54-61

Authors: O. Z. Мelnikova

It’s established that under the prolonged stress there were changes of geometric and spectral indices of the rats’ heart rate variability (HRV), manifestations of which depended on duration of stressful factors acting and represented the stress reaction development from the stage of anxiety to the exhaustion phase. Application of chlorpromazine at the beginning and against the background of stress blocked the central alpha adrenoceptors and contributed to renewal of the most HRV indices into the limits of control values at the end of experiment. The results of research show that the modulation of functional state of central noradrenergic system plays a great role in the changes of HRV during prolonged stress.

70-77

Authors: T. N. Mosendz, B. M. Mytskan

The information on histometric and electron microscopic study of the muscle fibers of the rats’ rectus femoris in the norm is presented. The basic types of muscle fibers and their composition are described. The composition reflects the close morphological interdependence of the structure of myoneural junctions and elements of muscular tissue. It may determine different patterns of both reactive and destructive processes in the skeletal muscles.

85-90

Authors: Y. Y. Nikulina, I. E. Sokolova, A. I. Vinnikov

The biological properties of the vaginosis pathogens isolated from 255 pregnant patients of the Pregnancy Pathology Department of the Mechnikov Dnipropetrovsk Region Clinical Hospital were studied. It was found that all investigated microflora was represented by the Candida yeasts, Trichomonas vaginalis, Toxoplasma gondii, Chlamidia trachomatis, Gardnerella vaginalis and Leptotrix vaginalis. The yeasts of genus Candida were the most common isolated pathogens of the vaginosis in pregnant women. The presence of Candida sp., Trichomonas vaginalis, Chlamidia trachomatis and Toxoplasma gondii in the vaginal smears taken from pregnant women was accompanied by changes of the immunity cell parameters and concentration of circulating immune complexes.

98-103

Authors: P. N. Polushkin, D. V. Galchenko, M. V. Nagovska

Modern methods of the pulse diagnostics were studied, the rationality of using different methods for pulse diagnostics was evaluated and the features of criteria interpretation of the pulse diagnostics methods and its scientific validation were revealed. The attempt of preparation of the pulse diagnostics data (human sphygmograms) for the further computer processing was made. The efficiency of preliminary results was assessed by the mathematical treatment of the sphygmograms. The attempt of prognostication of the reliability of possible variants of the mathematical treatment for the computer processing was made.

111-117

Authors: A. O. Ponedilok, V. G. Gavryliuk, Y. V. Khlopova, A. I. Vinnikov

The spectrum of causative agents of nonspecific infections of the women urogenital tracts is studied. It is established that the typical etiological agents of the vaginosis are yeast-like fungi Candida albicans (35.7 %) and Escherichia coli (30.2 %), and the clinical isolates of E. coli (47.3 %) and Proteus mirabilis (15.8 %) are usual for vulvovaginitis. The frequency of detection of the causative agents of inflammatory genito-urinary diseases in women of different age groups varies: strains of E. coli are often found in patients of 1–12 years (47.3 %) and in women of 43–66 years old (36.0 %), but C. albicans – in patients of 18–42 years (39.0 %). High levels of the resistance to penicilline, tetracycline and fluoroquinolone antibiotics in selected clinical isolates of opportunistic microorganisms are determined.

125-132

Authors: I. T. Rusev, V. D. Vinnik, D. A. Sokolovskiy

In 2005 highly pathogenic avian influenza spreaded rapidly from the Central Asia along the main migration routes of wild birds includingUkraine. In the autumn, and mostly in the winter, the avian influenza was found in many countries of Europe, Asia and Africa in the places of traditional birds wintering. The paper considers the ways of importation of the avian influenza pathogens intoUkraineand the role of wild birds in the possible formation of anthropogenic and natural foci of highly pathogenic avian influenza in megapolis conditions.

140-145

Authors: O. O. Shugurov

We studied the relationship of discharges phase characteristics in ascending column of spinal cord (SC) and specificity of activation of neurones, which generate negative components of evoked potentials of SC. The discharges was recorded from SC at a level of a presence of dorsal column (DC), spinocervical and dorsal spinocerebellar tract in upper lumbar and thoracic segments at a stimulation of a nerve or DC. It is shown, that the phase of the discharges depends on the quantity of synaptic delays in generating chain of such signals. Thus, the phase of a signal can carry the additional information on specificity of activation of the sensory elements in CNS.

152-161

Authors: A. G. Morenko

The study involved 104 healthy right-handed, 19–21 years old men withthe right leading ear. The electroencephalogram oscillation amplitude in α1 and α2 subbands and the amplitude-frequency characteristics of the superficial flexor and extensor muscles of fingers of both hands are analyzed during auditory stimulation and auditorymotor activities. Changes of the α1 activity power in men reflect the processes of nonspecific activation, α2– indicate the cognitive and mnestic mechanisms of the cortex control. Established dynamic shifts correlated linearly with complexity of the tasks. In accordance with the activation processes in the cortex supraspinal motor command formed and determined the amount of motor units included in the activity, the frequency and consistency of their discharges.