Visnyk of Dnipropetrovsk University. Biology, medicine

8-14

Authors: M. S. Goncharenko, G. M. Timchenko

The paper describes the scientific basis of a complex estimation of functional state of the children's organisms as a whole and their adaptive responses to living conditions (anthropogenic stress, social conditions of training) depending on age and biorhythmic type. The scheme of complex estimation for the children's adaptive capacities, including estimation of functional state and functional parameters, considers age and biorhytmological characteristics. That scheme is presented as a methodological basis of the research. The necessity of determination of children's functional state depending on their age and biorhythmic type to control circadian rhythms of physiological functions is proved. It indicates the stability of the endogenous biological clocks due to interactions of nervous, endocrine, lymphatic, cardiovascular, urogenital and digestive systems.

23-27

Authors: N. S. Dyrda, O. A. Dregval, N. V. Cherevach, A. I. Vinnikov

The strain variation B-2-6 with a higher production of spores (3.93 × 109 КFU/ml in that variation vs 1.94 × 109 КFU/ml in the initial strain) and endotoxin (10.1 mcg/ml vs 4 mcg/ml respectively) was obtained by means of the ultraviolet irradiation of the В-2 strain Bacillus thuringiensis spores. Insecticidal activity of the obtained variation is characterized by the deaths of 64.3 % of the great brown twist Archips podana at the 3rd day and 97 % at the 10th day after the treatment, which is 20.4 % higher than B-2 strain activity. Possibility of the obtained variation implementation for the natural plants protection against the leaf-eating insects is under discussion.

33-40

Authors: A. V. Zhukov, T. M. Konovalova

The soil mounds emerged owing to the mole rats’ digging activity have been shown to be characterised by less electrical conductivity than surrounded soil. This effect is due to the changes of the mounds bulk’s density and moisture. The effect of the mole rats’ digging activity on the soil electrical conductivity has been found not to be restricted by the geometrical border of the mounds. The mounds are surrounded by 1–1.5 m halo of increased soil electrical conductivity. The halo size is increased with the aging of the mound and with the compacting of their aggregation.

49-55

Authors: O. N. Korinovskaya, V. N. Gryshko

The sensitivity of 33 micromycete species to nitric compounds of copper, lead, zinc, nickel and cadmium has been determined. Absidia butleri Lendn, Mortierella vanesae Dixon-Stewart, Cunninghamella echinulata Thaxte, Curvularia tuberculata Jain, Cladosporium cladosporiodes (Fresen) G. A. de Vries and Fusarium solani (C. Mart.) Appel et Wollenw are sensitive to minimal content of the heavy metals (0.75 of maximum permissible concentration (MPC)) in the growth medium. At the same time Trixoderma longibrachiatiim Rifai, Alternaria alternatа (Fr.) Keissl and Penicillium sp. 4 demonstrated moderate growth under maximal concentration (50 MPC). It is determined that minimal content of the heavy metals in the initial stage of influence (up to 48 h) promotes growth of only Fusarium oxysporum E. F. Sm. et Swingle, while retards growth of the other species.

12-17

Authors: O. R. Bektascheva, K. V. Lavrentyeva, N. V. Cherevach, A. I. Vinnikov, А. А. Moskalenko

It was investigated the biological properties of 27 strains of Enterobacteriaceae isolated from 24 children with suppurative-septic infection from the Surgical Department of Dnieprodzerzhinsk Hospital № 7. Isolated microflora was presented by four bacteria species: Esсherichia coli, Enterobacter aerogenes, Klebsiella оxytoca and K. pneumoniae. Esсherichia coli was the most widespread agent of suppurative-septic infection for children. All bacteria were the most sensitive to fluoroquinolones ІІ generation (norfloxacin and ciprofloxacin) and cephalosporins ІІІ generation (cefotaxim and cefoperazon).

24-30

Authors: S. A. Goon’ko

Data on cadmium distribution in the soils of industrial, residential and recreational areas of Dniprodzerzhinsk city are presented. According to the classification of urban soils the following types of soils were identified in the city: urban soil proper, plantosoil, anthropogenic-surface-transformed natural soil and lawn soil. We have made the estimation of cadmium content. The problem of soils contamination in the city with high anthropogenic load is discussed. The total cadmium content in the industrial, residential and recreational soils of Dniprodzerzhinsk varies within 0.6–10.5 mg/kg, but movable forms make 0.1–3.4 mg/kg of soil. Cadmium in the urban soil proper of the right-bank city surpasses the maximum permissible concentration (MPC) twice. At the same time the soils in the left-bank area and in the eastern and western areas of the right-bank were under MPC.

35-39

Authors: K. V. Derkach, G. S. Krupska, O. E. Abraimova, T. M. Satarova

The effect of mineral components of nutrient medium and abscisic acid (ABA) on isolated immature maize embryos is investigated with the aim to intensify their growth and to inhibit their early germination. Abscisic acid together with MS medium leads to the stasis of the development of both seedlings and embryos. The application of ABA along withN6 minerals inhibited the development of seedling’s organs in favour of embryos growth. For production of seedlings from immature embryos of 1 mm long their cultivation on modified N6 medium with addition of 12 mg/Lof abscisic acid is recommended.

47-53

Authors: A. L. Drozdov, E. M. Demchenko, A. Ejjyad, O. P. Nerush

Data obtained showed that using of neuroleptics in conditions of experimental psychosis was accompanied by the decreasing of mobility, investigative behavior and emotionality in experimental animals more than in intact ones. Asopposed to that, the action of atypical antipsychotic drug – sulpiride was characterized by the anxiety decreasing but the mobility increasing. The nootropic agents resulted in increasing of motor and emotional activity. The established pharmacological effects of neuroleptics, antidepressants and nootropic agents may use in clinical practice for treatment optimization of the patients with mental disability including schizophrenia.

59-67

Authors: G. S. Kononova, S. V. Antonuk, N. I. Shtemenko

Decrease of proliferative activity of the cells of Guerin’s carcinoma T8 in the different conditions of administration of the Rhenium-Platinum anti-tumour system as nanolyposomes is shown. The Rhenium-Platinum anti-tumour system influenced on the morphological indices of tumour tissue. The cluster rhenium compounds reduced the indices of pathological mitoses 3.0–3.6 times in comparison with a control group. The amount of PCNA-positive cells under the Rhenium-Platinum system treatment went down by 82.5–84.5 %. The anti-tumour system led to the predominance of apoptotic cell death over the necrotic one. The most effective agent was the compound with cis-adamantan organic ligand cis-Re2(C10H15COO)2Cl4·2DMSO introducted on the 9th day.

75-80

Authors: R. A. Novitsky, O. A. Khristov, V. N. Kochet

Data on ichthyopathological examinations of mature fish (pike, bream) from Dniprovs’ke reservoir are presented. That fish were confiscated from a person used the illegal homemade electric instrument ("electrical fishing rod"). All explored specimens of breams had keratopathy and visceral injury of different level. The most significant injuries were noted for kidney tissues. There was found the hemoperitoneum as well as nephremorrhagia and cerebral hemorrhage. In pikes it was noted the hemorrhages of hyomandibular canal. Determined ichthyopathological effects can be used for clear diagnostics of electric current lethal impact on fish.

88-94

Authors: O. Z. Melnikova, V. P. Lyashenko, T. G. Chaus, D. A. Burceva, L. V. Kalyuzhnaya

The dynamics of power and waves spectral composition of total background electrical activity in hippocampus and changes of motor and investigative activity of rats during long-lasting stress were investigated. The dependence of analysed indices on duration of the stress influence was found. The correlation between hippocampus bioelectrical activity and behavioural reactions of rats were established.

101-106

Authors: Y. Y. Nikulina, K. V. Lavrentyeva, N. V. Cherevach, О. А. Sirokvascha, A. I. Vinnikov

It was investigated the biological properties of 494 strains of Staphylococcus genus isolated from pathological material of patients from 15 Departments of Mechnikov Dnipropetrovsk Region Clinical Hospita. All microflora was presented by four bacteria species: Staphylococcus aureus, S. epidermidis, S. haemolyticus and S. saprophyticus. S. aureus was the most extended agent of infections. All bacteria were the most sensitive to linezolid, gentamycin and meropenem.

114-119

Authors: Y. S. Sukharev

IFA test-sistem is developed for identification of the heat stable (ST) enterotoxin of Escherichia coli in the calves faeces at different stages of colibacteriosis. The range of its titre makes ≥ 250 ng/ml at the hyperacute stage, at the acute stage – ≥ 500 ng/ml, and at the subacute one– ≥ 1 000 ng/ml. The registered enterotoxin titres can be considered as the diagnostic indices.

125-129

Authors: O. O. Shugurov

The electrical evoked potentials (EP) of spinal cord (SC) in reply to the mechanical activation were investigated. The energy of stroke variated from 1·10–3 up to 4·10–3 J. The registration of EP is realized in a L5 segment of SC, the point of stimulation was sequentially moved upwards along the SC. We have shown, that the augmentation of stimulus force produced the increase of amplitude of a potential and its duration. After excluding a place of stimulation from a place of registration the potential was decreased proportional to the square of the distance. We concluded that the mechanical percussions of the SC resulted in the development of long-term oscillating activity of neurones of SC.