Journal of Advanced Biotechnology and Experimental Therapeutics

09-19.

Authors: Md Shamim Akhter

Growing functional information regarding bioactive TLQP-21 has led to the ubiquitous demand for identification of receptors associated to the peptide, which resulted in the first wave of murine TLQP-21 receptors, gC1qR and C3AR1. gC1qR was identified as receptor of TLQP-21 using chemical crosslinking and monomeric avidin column purification following by MS analysis. TLQP-21 responsive CHO-K1 cells were used to search for its receptor, C3AR1. Putative GPCRs, which may partake in regulating intracellular biological functions induced by TLQP-21, were indexed after the CHO-K1 cellular transcriptome was sequenced using unbiased Genome Wide Sequencing. TLQP-21 binding in the cells were found to be reduced by the gene knockdown with the siRNAs targeting C3AR1. C3AR1 antagonist, SB290157 was shown to prohibit TLQP-21 activity in CHOK1 cells. But this finding was not demonstrable in human cell line. The differences of human TLQP-21 sequence with that of murine TLQP-21 explains the poor binding of the human orthologue with its corresponding receptor. This may suggest a different set of receptors when considering human and rodent variants of TLQP-21. The identification of HSPA8 as receptor was performed using affinity based chromatography and mass spectrometry from human SHSY-5Y cells. Molecular studies in silico revealed that the peptide binding pocket in HSPA8 is an appropriate fit for TLQP-21 docking. Cross-linking and FACS methods presented the TLQP-21 binding to cells from the SHSY-5Y line. The establishment of HSPA8 as a putative receptor for human TLQP-21 can be exploited to explore new horizon in diagnosis and therapies for VGF related human diseases.

29-42.

Authors: Hung Ngoc Hoang, Son Hung Lan Nguyen, Cu Nguyen

Combining use of various methods of bird study from line transect observations, birds capture and release by mist-nets, collection of specimens from local people and analysis of samples kept at Pu Luong Nature Reserve (NR) office throughout the time from 2015 to 2018 and inherit the results of previous studies, we provide an updated list of 252 species of bird belonging to 58 families and 15 orders from Pu Luong NR, Thanh Hoa Province, North Vietnam.. A total of 216 species of birds were recorded during four years. In which, 74 species are reported for the first time from this nature reserve, and 41 species are first records for the avifauna of Thanh Hoa Province. Additional, the data of distribution characters of avifauna in each study site are also provided. According to the International Union for the Conservation of Nature (2019), five species were in the Near Threatened category and three species were in the vulnerable category. Compared to 6 other National Park (NP) and NR in the North Central Coast of Vietnam, the avifauna in Pu Luong NR is close to the avifauna in Pu Hu NR. There are 89 common bird species recorded in all 7 NP and NR, while there are 15 species recorded only in Pu Luong NR. Birds are considered as good bio-indicators of the degree of human disturbance to the forest ecosystem. Observing changes in bird community can assess the status of the forest in the future.

49-55.

Authors: Md. Rezaul Karim, Md. Foyzur Rahman, Md. Rezanur Rahman, Zinat Tamannaa, Dipayon Krisna Ghose, Md. Nurul Islam, Md Tofazzal Hossain, Rokibul Islam, Md. Ataur Rahman

Oyster Mushroom (Pleurotusostreatus), an edible mushroom, is traditionally being used as a curing. However, hypoglycemic as well as antidyslipidemic effects on methanolic and ethyl acetate extracts of oyster mushroom in streptozotocin-treated diabetic rat model was not investigated yet. Here, we investigated that oral administration of 200 mg/kg oyster mushroom for 30 days were estimated through evaluating the fasting blood glucose level and blood lipid profile of diabetic rat. The results from our studies explained that P. ostreatusoyster mushroom considerably decreased blood glucose level initiated after treatment on second week. However, oyster mushroom was significantly reduced in plasma triglycerides (TG), total cholesterol (TC), as well as low-density lipoprotein-cholesterol (LDL-C). Additionally, high density lipoprotein-cholesterol (HDL-C) was enhanced by the treatment of oyster mushroom. Therefore, the current study suggests that P. ostreatus has antihyperglycemic and anti-dyslipidemic effect on streptozotocin-induce diabetic rat model.

59-69.

Authors: Asif Shahriar, Anina Tul Kobra, Farzana Yasmin Shomi, Talha Bin Emran, Jewel Mallick, Mycal Dutta

This investigation was carried out to study the extremely diversified characteristics of rhizobial strains isolated from locally produced legumes of different varieties. The rhizobial isolates designated as S 1, S 2, S 3, S 4, S 5 and S 6 were purified and subjected to various morphological, cultural, antibiogram and biochemical and plasmid profiling to unmask their diversified nature to some extent. The study revealed that the Sesbania strain (S 2) possessed extraordinary properties for most other strains. Though the strain is allocated in the slow-growing group, it showed an atypical fast growth rate, and also it produced a profuse amount of exopolysaccharide (EPS)/ lipopolysaccharide (LPS). The Arachis and Vigna strains also showed a faster growth rate than usual, whereas the rate of EPS/LPS production was profuse in the cases of Vigna and Lens strains. This rate was noted low in cases of Pisum and Arachis strains, while Phaseolus produced a moderate amount of EPS/LPS. Like Sesbania, the Pisum strain also absorbed Congo red dye strongly and including Sesbania, all the other strains ware catalase-negative, which was an unusual property of rhizobial strains. Antibiogram profile of the test strains was done to retain experimental strains after plant infection test, as overlapping host range is a common phenomenon in symbiosis. The antibiogram profiles showed that more or less all the strains were resistant to Ampicillin, Bacitracin, and Amoxicillin. Phaseolus strain was intermediate to Bacitracin, and Pisum strain was susceptible to Ampicillin, which can be related to loss of the plasmid. In plasmid profiling of the strains, several numbers of plasmid DNA band was observed under UV transilluminator after gel electrophoresis. In the present study, rhizobial species from different leguminous plants were detected, and plasmids were cured of the cell using acridine orange to determine their effect on Rhizobium spp. for antibiotic resistance. The findings of the research point out the diverse nature of rhizobial isolates with specific reference to the strains infective on Sesbania aculeate, which poses problems to characterize strains infective of different legumes.

77-83.

Authors: Fahmida Shifah, Abu Montakim Tareq, Mohammed Aktar Sayeed, Mohammad Nazmul Islam, Talha Bin Emran, Md. Ahsan Ullah, Muhammad Abdul Mukit, Ahmed Ullah

The study reports the in vivo antidiarrheal and in vitro cytotoxic and thrombolytic activities of methanolic extract of Hedychium coccineum leaves (MEHCL). The antidiarrheal activity was evaluated by castor oil-induced diarrhea, whereas the intestinal motility by charcoal marker. In addition, brine shrimp lethality bioassay and human blood clot lysis were used to evaluate the cytotoxic and thrombolytic activities, respectively. In antidiarrheal study, castor oil-induced diarrhea and gastrointestinal motility exhibited a significant dose dependent reduction in diarrhea and defecation and an extremely significant (P < 0.0001) inhibition in intestinal motility and peristalsis index by 200 and 400 mg/kg of MEHCL. The brine shrimp lethality bioassay revealed a considerable cytotoxic effect of MEHCL (LC50= 81.59 µg/mL; R² = 0.927) while in thrombolytic a significant percentage of clot lysis (17.36%, P < 0.01) demonstrated. The findings suggest that H. coccineum leaves could be potential sources for biological activity.