Journal of Clinical and Experimental Medical Research

5

Authors: Sukhareva V. A., Garbuzova V. Yu., Ataman A. V.

C677T polymorphism (rs 1801133) of methylenetetrahydrofolatereductase gene (MTHFR) in 124 healthy individuals was determined. It was shown that the ratio of major homozygous allele (C/C), heterozygotes (C/T) and homozygotes for the minor allele (T/T) was 46 %, 48.4 % and 5.6 %. Frequency of individuals-carriers of the minor allele (C/T+T/T) among men was higher than among women (P = 0.061). Female carriers minor allele (C/T+T/T) growth was significantly lower, and BMI was higher compared with women who were homozygous for major allele (C/C) (P < 0.001, P = 0.034 respectively).

5

Authors: Kasatkina S. G., Panova T. N., Kasatkin S. N.

We examined 56 patients (12 men and 44 women) aged 45 to 60 years (mean 51.13 ± 5.32 years) in order to study the dynamics of indicators of endothelial dysfunction in patients with type 2 diabetes with subclinical hyperthyroidism on the background of the natural course and treatment thyrostatics. All patients were divided into two subgroups: the first subgroup received treatment (20 patients) with tyrosol; a second subgroup of dynamic monitoring (36 patients) did not receive treatment during follow-tyrosol. The results indicate an increased risk of cardio-vascular diseases for patients with type 2 diabetes with subclinical hyperthyroidism and require timely active preventive measures.

5

Authors: Prystupa L. N., Murenets N. A., Dominas V. M., Kyrychenko N. N., Shkandala A. Yu.

We determinated increase of C-reactive protein and proinflammatory cytokins (IL-6, IL-8, TNF-α) in patients with chronic obstructive pulmonary disease II and III stages. Application of atorvastatin in addition to basis therapy in patients with chronic obstructive pulmonary disease enhanced the level of anti-inflammatory treatment effectiveness by: faster decrease of C-reactive proteins and proinflammatory cytokins levels. Using these data we can recommend statins to eliminate inflammation and slow the disease course.

7

Authors: Arkhypkina T. L.

The study involved 35 patients with polycystic ovary syndrome and obesity. According to the basal secretion of аnti-Müllerian hormone, all patients were divided into two groups: the first group consisted of 20 women with index >10.3 ng / ml (14.1 ± 0.5 ng / ml) and the second – 15 women with index < 10.3 ng / ml (9.2 ± 0.2 ng / ml). I noticed similar changes in both groups after six month of therapy with metformin: weight loss, decrease of insulin, luteinizing hormone, testosterone and increase sex hormone-binding globulin, which was accompanied by reduction of free androgen index. Besides, the number of antral follicles and volume of ovarian reduced, menstrual cycle normalized. Perhaps, pregnancy occurred only among the representatives from the second group. I assumed that the basal secretion of аnti-Müllerian hormone could be a predictor of clinical effectiveness of treatment with metformin.

8

Authors: Tsema Ye. V.

The article is devoted to discuss the results of radical surgical treatment of 57 patients with recurrent pilonidal disease. Patients with pilonidal disease were divided into the control and main groups. Thus, 31 (54.4 %) patients composed the control group of patients who were treated by wide excision with wound closure, while 26 (45.6 %) patients composed the main group of patients who were treated by Bascom's procedure (cleft lift procedure). The results of treatment patients were following for 1–3 years. There were 23 (74.2 %) complications in patients of the control group: wound abscess (16.1 %), primary wound dehiscence (16.1 %), secondary wound dehiscence (19.4 %), wound hematoma (12.9 %) and repeat recurrent of disease (9.7 %). There were 3 (11.5 %) complications in patients of the main group: wound abscess (3.8 %), secondary wound dehiscence (3.8 %) and wound hematoma (3.8 %). There was no repeat recurrent of disease after Bascom's procedure. The using cleft lift procedure (Bascom's procedure) permits essentially decrease (χ2 = 22.4; Р=0.001) frequency of postoperative complications in patients with recurrent pilonidal disease.

8

Authors: Vlasenko M. A., Rodionova Yu. V.

Myocardial dyssynchrony is important part of the etiopathogenesis of heart failure. Diagnostic criteria and methods for determining the dyssynchrony are not enough investigated. We analyzed various types of dyssynchrony and discussed modern parameters to diagnose them. The authors proposed a unified approach to identify dyssynchrony in patients with heart failure combined with diabetes mellitus type 2. There were determined the diagnostic sensitivity and specificity of the basic ECG and echocardiographic parameters in the diagnosis of dyssynchrony. We developed criteria to diagnose dyssynchrony using ECG and echocardiographic routine markers. The proposed diagnostic algorithm enables to diagnose dyssynchrony highly reliably.

10

Authors: Bushtruk A. N., Tkach G. F., Pogorielov M. V., Sikora V. Z.

The article is devoted to study toughness of rat’s humeri testing them for stiffness, strength and stretching. Our aim is to create a 3D-model of the long bone for rats of different age. This model will allow conducting numerous toughness tests by changing the initial parameters. The portable experimental unit was used to test humeri stretching, with the measuring scale accurate to 0.25 kg. Evaluation of toughness was carried by Pro/Engineer software package (PTC, USA) and included Pro/Mechanica modulus. The minimal value of critical toughness was measured in the bones of sucking rats. Value of stretching showed almost directly proportional dependence between critical bone toughness and rat’s age. Having compared experimental data and calculations of crack load values, we pointed that value deviation is less than 7 %. The 3-D image of the long bone for rats of different age can be frequently used for various deformation influences and with different initial parameters of the bone tissue.

3

Authors: Khyzhniak M. V., Potapov A. A., Pryymak E. V.

Neurovisualization methods estimate the degeneration degree of the intervertebral disc regarding to its structure, differences in borders of the nucleus pulposus and annulus fibrosus, signal intensity from the intervertebral disc in the T2-mode and its height. Assessment of neurovisualization methods refines the indications to use puncture treatment.

6

Authors: Reznichenko N. Yu.

The aim of the investigation was to determine the changes in metabolism: daily changes in prooxidant and antioxidant systems, functioning of cell membranes and the state of endogenous intoxication in patients with psoriasis and healthy men of different age. I examined 28 men aged 45-64 years, who suffer from psoriasis, 40 healthy men aged 45-64 years, 35 healthy men aged 25-44 years. For the first time it was found that the dicrease of diurnal fluctuations in activity of peroxide oxidation of lipids, the growth of its overall activity, inadequate response of antioxidative system occured; violations of the functioning of cell membranes were marked and the growth of endotoxicosis occured in men with increasing age. The system of antioxidant protection almost did not respond on the diurnal activity of lipid peroxidation in men of older age groups, who suffered from psoriasis. I observed the decrease of resistancy of membranes and the increase in manifestations of endotoxicosis. Those were pathogenetic links of the disease. I proved the necessity to take measures aimed at improving metabolism in men of older age groups.

6

Authors: Smiyan A. I., Mozgova Yu. А., Moschich O. P.

The objective is to study the features of the intestinal microflora in children of teenage age with chronic tonsillitis and secondary cardiomyopathy. The article describes the principal features of the microbiota of the colon in children between 13 and 18 years, patients with chronic tonsillitis and secondary cardiomyopathy. We have found that the microbiocaenosis intestinal disorders had certain qualitative and quantitative changes of its microflora, for example: reduction of bifidobacteria, lactobacilli and Escherichia coli with normal enzymatic activity; growth of fungi Candida; and a significant increase of staphylococci, streptococci and pathogenic microflora. These changes have happended during the acute disease period. At the same time, changes were more significant in the children with secondary cardiomyopathy with chronic tonsillitis and colon microbiota in contrast to the patients with chronic tonsillitis without cardiac pathology, which manifested as significantly smaller quantity of lactobacilli (p < 0.001) and greater quantity of fungi family Candida (p < 0.05).

7

Authors: Tseluyko V. I., Yakovleva L. N., Kuznetsov I. V.

The purpose of research was to study the possible link among traditional risk factors (RF) and polymorphism of endothelial NO-synthase (eNOS), angiotensin-converting enzyme (ACE) and angiotensin II receptor type 1 (AT2R1) with the prevalence of atherosclerosis in patients with ischemic heart disease (IHD). We examined 120 patients with IHD (108 men, 12 women) with stable angina of II-III functional class and the presence of hemodynamically significant coronary arteries (CA) stenoses, according to selective coronary angiography. Examinations of the patient group, except conventional ones, included determination of ankle-brachial index and Doppler ultrasound of the lower extremities and carotid artery with a quantitative intima-media thickness assessment. According to test results, patients were divided into two groups: I group consisted of 55 patients with evidence of atherosclerotic vascular disease in two and three basins, II group – 65 patients with only CA atherosclerotic lesions. T-786S eNOS gene promoter allelic polymorphism, insertion-deletion (I / D) polymorphism of the ACE gene and polymorphism A1166S of AT2R1 gene were examined with the help of polymerase chain reaction. We performed statistical analysis using statistical software package «Statistica 8.0» (StatSoft Inc, USA), Microsoft Office Exel-2003. For comparison of quantitative traits Student's t test was used, for qualitative traits – Pearson's χ2 test. We conduct stepwise multivariate regression analysis to identify causal relationships between the studied parameters. We found, that other vascular basins, along with CA, are involved into atherosclerotic process for 48.5 % of patients with IHD. It was established, that patients with IHD and multilokus atherosclerotic lesions significantly frequently are carriers of eNOS gene T-786S polymorphism and AT2R1gene A1166S polymorphism mutant alleles than patients with CA lesions only. We proved the independent correlation between the development of advanced atherosclerosis in patients with IHD and such indicators as smoking, type II diabetes, family history of cardiovascular disease early onset, multivessel CA lesions and the presence of eNOS gene T-786S polymorphism C allele.

7

Authors: Bobrysheva I. V.

I conducted the experiment on 180 white outbred male rats from three age periods (pubertal, reproductive and of expressed age-related changes) with the use of histological and morphometric methods. It was followed by statistical analysis. I pointed out that experimentally simulated activation of the immune system caused the morphological remodeling in the animal spleen. The manifestations were: increasing the area occupied by the white pulp and the size of the lymphoid follicles, widening the marginal zone and forming secondary follicles containing germinal centers. The highest level of spleen reactivity in response to the imunofan was observed in pubertal animals. Changes in the spleen white pulp of rats with expressed age-related changes took place against the background of involutive processes.

7

Authors: Sikora V. Z., Boyko V. O.

We pointed out that microelementosis affected the body and led to changes of the salivary glands due to the result of histological examination of rats’ submandibular salivary glands. These effects were: decrease of functional activity, disruption of the compensatory-adaptive mechanisms and reactions, structural disorders. Besides, it had influence on the correlation between extension of experiment duration and significant structural changes against microcirculation disorders.

9

Authors: Smiyan O. I., Bynda T. P., Dmitrova Ye. V., Sukharieva V. A.

The article analyzes issues of etiology, pathogenesis, clinical manifestations and diagnosis of acute viral respiratory infections in children. We have determined the main causes of acute viral respiratory infections, mechanisms of pathogenesis, clinical and diagnostic manifestations of the disease course from the literature.

12

Authors: Sikora V. Z., Yarmolenko O. S.

This article analyzes the recent literature on the age features of the structure and function of the intact heart muscle, as well as on the issue of heart remodeling in experimental animals of different ages under the influence of damaging factors. The myocardium undergoes morphological changes, with age, that occur as the restructuring of parenchymal and stromal components of the heart muscle. We point out the increase in mass of the heart and enlargement of its chambers with preferred hypertrophy and dilation of the left ventricular cavity during the aging process. The number of cardiomyocytes decreases throughout life. Quantitative changes of the cardiac cells are compensated by their hypertrophy. Reduction of the total volume of cardiomyocytes is accompanied by an increase of volume of connective tissue. The characteristic feature of aging is the accumulation of myocardial collagen in the interstitial space, exemia. The term "cardiac remodeling" includes the changes of both cellular and stromal components, volume of chambers of the heart cavities, which develop at pressure or volume overload, under the influence of various endogenous and exogenous factors. These lead to a structural reorganization of the heart muscle function. All chambers of the heart are remodeling. A significant number of clinical and experimental studies structural and functional reorganization of the heart under the influence of external and internal factors. However, the mechanisms of age-related changes in the myocardium at all levels of its organization structure are still questioned.

4

Authors: Markevych O. V.

This article is devoted to study the changes of the rat’s bone tissue of different age groups influenced by the radiation low doses. It is proved that application of «calcium - gluconate» provides significant decrease of osteoporosis especially at a young age, while the rogression of osteoporosis is slowed down in the old rats.

4

Authors: Popov S. V.

The aim of the investigation was to study maturity suspected dysplasia of the hip. 75 children (aged till 1 year) were examined by ultrasound diagnostic method. The slowed hip formation at 81.33 % of patients is revealed, the broken formation - at 2.67%. Normalization of ultrasound data takes place mainly during in the second half of the year.

4

Authors: Matyash V. I., Ralets N. V., Berezina L. V.

The article presents survey data on 142 patients with meningoencephalitis using fotopletyzmography. We prove that the violation of the autonomic nervous system is the pathogenesis chain meningoencephalitis affect the severity of the pathological process. Besides, it is also the objective method for assessing the severity and the criterion of dynamic observation of the patient.

5

Authors: Spivak J. S., Bondarenko V. O., Miroshnichenko A. V.

The article is devoted to study the semen haracteristics, levels of testosterone in the infertile men with autoimmune thyroiditis, and in the patients with pathospermia against chronic prostatitis before and after therapy with CARDONAT, vitamin E and tsefasel. It is shown that this treatment increases mobility and improves viability of spermatozoa. It is found that treatment reduced sperm agglutination; it coincides with a reduction of antibodies to tyreoperoxidase in the infertile men with autoimmune thyroiditis. It is proved that the intended therapy has not affected the functional state of the thyroid gland and the secretion of testosterone by the testes in the patients with euthyroid state and normal quantity of testosterone in the blood.

5

Authors: Zhornyak О. I.

The research results of antimicrobial agents’ effects on the adhesion of gram-positive and gram-negative microorganisms were described. The studies have shown that antiseptics (such as septefril, sebidin, adzhysept) inhibited adhesion of bacteria. The lowest index of adhesiveness observed in the presence of septefril.

5

Authors: Rudenko T. M.

The article proves the effectiveness of the ursodeoxycholic acid drug application for the non-alcoholic steatohepatitis treatment in the patients with hypertension and obesity. Application of the ursodeoxycholic acid drug accelerates normalization of clinical manifestations, normalization of biochemical parameters of liver function and lipid metabolism, reduces sonographic phenomenon of the fatty liver infiltration. The positive clinical effects and positive dynamics of biochemical and sonographic indicators confirm enhancement of metabolism in the liver cells in the majority of patients. These factors may recommend the ursodeoxycholic acid application for treatment and prevention.

5

Authors: Suhonos V. A.

This article presents comparative characteristics of structural and functional parameters of the trunk vessels and clinical laboratory indices in the patients with stage II hypertension with different body mass. It is determined, that hyperinsulinemia and systemic inflammatory response factors of C-reactive protein and interleukin-6 take part in vascular remodeling mechanisms and arterial hypertension progression in the patients with normal body mass and with the obesity.

5

Authors: Korytskyy G. I.

A high prevalence of endocrine diseases in children and adolescents with simultaneous tendency to reduce the incidence is revealed in Ternopil region by a comprehensive analysis of the dynamics of basic health indicators - prevalence, incidence, its structure in children up to 14 years and adolescents. These indicators show the need for a qualitative examination of children with active detection of diseases of the endocrine system of primary health service - family physicians, pediatricians.

6

Authors: Slisarenko O. V.

Reparative bones regeneration peculiarities under cellular dehydration were researched on aged laboratory male rats. The shin bone was fractured under cellular dehydration modeling. From the commencement of the experiment the percentage composition of regenerate cells changed. Increasing dehydration caused more changes. Further dehydration conditions resulted in isregenerative changes and tissue structure inhibition, which is evident due to the remains of the granulation tissue on the 15th day under the medium and high dehydration levels and the remains of the fibroreticular tissue – on the 24th day under the high dehydration level. The changes were not detected in the control set of animals. These changes were confirmed with the raster electron microscopy. One can also observe the reduction of the basic chemical elements, hardness disturbance, and general growth inhibition. The result of these changes is general inhibition of callus formation.

6

Authors: Kulikova M. V., Ascheulova T. V

The article is devoted to study carbohydrate metabolism characteristics and identify the I/D gene polymorphism of the angiotensin-converting enzyme in the patients with arterial hypertension. This arterial hypertension is accompanied with a type II diabetes mellitus. We examined 103 patients with arterial hypertension; they were randomized into two groups distinguishing either they preserve or not the type II diabetes mellitus. During the research we have figured out that the majority of patients from the both groups were the carriers of D-allele angiotensin-converting enzyme gene (the patients with DD and ID genotypes). The patients with arterial hypertension and unfavorable DD and ID genotypes had significant worsening of carbohydrate metabolism characteristics, such as insulin and HbA1c. It can be regarded as the proof of a bad prognosis about possible the type II diabetes mellitus development if the D-allele angiotensin-converting enzyme gene is carried. Existence of ID genotype caused body-weight and arterial pressure increasing. We have pointed out essential differences among systolic pressure, diastolic pressure, body-weight index and insulin in the patients from the 2nd group (the subgroup with a manifestation of ID genotype). These proved the complicated character of arterial hypertension course, which was accompanied with the type II diabetes mellitus.

8

Authors: Korepanov A. L.

Adaptive potential correlations of the adolescents with different physical development were figured due to the results of analyzed physio-metric, functional and energy-supply indexes. Early developers had lower adaptive potential comparing to the adolescents of the same age. The article provided information about the correlations between interchange of energy and aerobic potential, on the one hand, and the essential figures of physical development, on the other hand.

8

Authors: Vynnychenko L. B., Holovko T. M., Bezsmertna R.V.

The aim of the investigation was to study the peculiarities of the course and multifunctional condition of myocardium in patients with chronic cardiac insufficiency (CCI) on the background of ischemic heart disease (IHD) in combination with chronic obstructive pulmonary disease (COPD). 134 patients aged from 45 to 78 were supervised, with CCI of functional class (FC) II and III of ischemic origin. The main group consisted of patients with CCI of FC II and III with concomitant COPD of II and III stages (n=74). The control group was represented by patients with CCI of FC II and III without impaired external respiration function (ERF). We estimated the structural and functional parameters of cardiovascular system by echocardiography method, we also took into account life quality of the patients, dyspnea intensity degree by the developed scales, biochemical and clinical blood analysis, ERF investigation and saturation of blood by oxygen. The investigation results showed life quality deterioration in all investigated patients (according to Minnesota questionnaire) and in patients with combined pathology it was reasonably lower. The basic factors of cardiovascular risk which significantly differred the group of patients with CCI of ischemic origin in combination with COPD from the isolated one were tobacco smoking and Arterial hypertension. The study of concomitant COPD comparing to the patients with CCI without COPD pointed on the significant increase in the right atrium (RA), right ventricle (RV), mean diameter of pulmonary artery (MDPA) size. Besides, we noticed other features, such as arterial hypertension was more frequent, left ventricular hypertrophy (LVH), predicted unfavorable types of LV remodeling (concentric and eccentric), the number of people with “delayed” type of left ventricle diastolic dysfunction (LVDD) at the expense of increase in number of patients with intermediate “pseudonormal” LVDD disturbance. At the equal FC of CCI, the patients with concomitant COPD comparing to the patients with CCI without COPD, have more increased volume of haemoglobin and erythrocyte.Thus, with the signs of the right parts of the heart increase in patients with CCI on the background of IHD with preserved systolic function of the left ventricle. We can assume concomitant COPD and continue the investigation to specify the diagnosis.

9

Authors: Bereznyakov I. G., Makharynskа O. S., Doroshenko O. V

Issues to determine community-acquired pneumonia in the patients with type II diabetes mellitus are considered in the article. The clinical course is analyzed.

3

Authors: Pobedenniy A. L.

The content of main pro- and anti-inflammatory cytokine in serum of the patients with traumatic brain injury immediately after surgical operation and 10 days later was studied in the article. Concentrations of IL-1β, IL-2, IL-6 were increased significantly without elevation of IL-4 and γ-IFN. Ratio IL-1β/IL-4 was increased too. After 10 days of usual therapy we observed absence of their dynamics. It was a basis for the immunocorection.

4

Authors: Khyzhnyak M. V., Potapov O. O., Bodnarchuk Y. A.

Uncomplicated fractures of the thoracolumbar region are widespread pathology. These kinds of spine injuries represent over half all spine fractures. There are modern classifications of spine injuries and their biomechanical structure. The three column classification of Denis was developed to show the injury mechanism and its further treatment. Patients mostly complain of severe local back pain and radiculopathy. Results of puncture percutaneous vertebroplasty (PPVP) method in surgery were given. Under the experiment were 55 patients in their 51+1.2 years suffered traumatic compressive fractures of vertebral bodies. We have operated 55 patients. Pain syndrome strength was evaluated with Visual Analogue Scale. Such positive postoperative dynamics as pain syndrome regress and patient’s quality of live improvement were noticed. Thus, PPVP is considered to be minimally invasive and extremely effective method of traumatic compressive vertebral bodies’ fractures treatment.

5

Authors: Potselyev V. I.

The article is based on decadelong monitoring of 123 general-family practice institutions located in the rural area. It was pointed that medical care quality improving caused by: diagnostic equipment completeness, certain day patient facilities number and capacity enlarging, geographically-territorial accessibility reforming, normalization of population general number regarding to the certain institution. Obtained data dealt with significant changes caused by geographically-territorial accessibility, ambulatory completeness of a general practitioner and the new qualimetry approach usage. Thus, the approach can be involved into similar analyzes, Health Care System quality monitoring and managerial decision evaluation of effectiveness.

5

Authors: Lukavenko I. M., Andrjushenko V. V., Kononenko M. G., Yazykov O. V

We studied 10 samples of histological material (mammary gland tissue) from 6 women with precancerous breast pathology. The levels of estrogen, progesterone, mammaglobin receptor's expression were defined. The same histological structure appeared nonhomogeneous according to immunogistochemical test-results. This may be representative the characteristics of the hormonal status and the age of patients, and also be a manifestation of breast cancer and an indicator of a predisposition to it.

5

Authors: Kutya S. A., Schvets T. A., Melnik M. V.

Article is about anatomists, who were papal physicians: M.R. Colombo (c.1515-1559),G.B. Canano (1515-1579), G.C. Aranzi (1530-1589), C. Varolio (1543-1575), A. Cesalpino (1525- 1603), M. Malpighi (1628-1694), G.M. Lancisi (1654-1720) and G. Flaiani (1739-1808). Their contribution into anatomy and brief biographical information are given.

6

Authors: Gafarova Ye. A., Pikalyuk V. S., Kryventsov М. А

The article deals with the liver structural changes of experimental rodents of juvenile age, who were under 10- or 30-times hypergravitation influence. These changes mostly have reversible character and are accompanied by adaptive-compensative mechanism activization. They are directed to reduce negative influence of exogenic factors. Both structural-functional changes and adaptive reaction intensivety dominate in the rodent subgroup, which got more influence. Obtained gysto- and cytomorphological data prove positive adaptive response establishment on cellular level. First of all this positive adaptive response is characterized by cell quantity increasing of different mitosis stages. Most obvious histopathological changes were found in the mature rodent subgroup, which are characterized by cytomorphological traits of the vacuolar degeneration and non-adequate adaptive response realization. However, more obvious negative influence of the overload was pointed in the longer term experiment (30-times hypergravitation influence)

6

Authors: Kryvoshey A. V.

The aim of the article is devoted to study microcirculation flow disorder and peripheral hemodynamics in children, who have responsive arthropathia. There were given data concerning peculiarities and correlations between microcirculation and peripheral hemodynamics under responsive arthropathia in children. Microcirculation functional changes were pointed out. These changes dealt with influence on both flow distribution components and exchanging, draining components. It was pointed that hemodynamics disorders in proximal area of the hurt joint had compensatory character, while the distal area obtained decompensatory character. There was direct correlation among responsive arthropathia disease severity, ill person age, microcirculation flow disorder level and peripheral hemodynamics. Thus, disease severity worsening and age increasing leaded to severity of microcirculation and hemodynamics disorders.

6

Authors: Ospanova T. S., Zaozerska N. V.

The scope of the article was to study expression of adhesion molecules and screen respiratory functions in the patients with diabetic nephropathy of different chronic renal disease progression. Interaction between on the one hand: E-Selectine molecule levels, sICAM, sVCAM and MAU, proteinuria of first-state chronic renal disease, and on the other hand respiratory functions and creatinine of third-state chronic renal disease. Obtained data will be useful for early prognosis of renal irritation and pulmonary affection signs of diabetes mellitus.

8

Authors: Bessalova Ye. Yu., Pikalyuk V. S., Korolev V. A

The article is devoted to study biological effects of cerebrospinal fluid of lactate cows with its injection into female and male white rats as a xenogenic medicine. We studied the reproductive systems by physiological and morphological methods. We pointed significant, but reversible inhibitory effect of cerebrospinal fluid derived during physiological anovulation of a donor, we analyzed this effect of the recipient’s reproductive system. Inhibitory effect of cerebrospinal fluid on gonad was identified both in male and female organisms. This effect is reversible and dependable on recipient’s age and cerebrospinal fluid frequency of administration. Thus, the peak changes were observed during cerebrospinal fluid injection in perinatal period and numerous injections. There were the following changes: mating number and sexual motivation reducing; dynamic of estrous cycle and phases of cycle duration; female’s fertility decrease and temporary male infertility; testicles and ovary weight reductions, reproductive hormones level in blood serum, anovulation was caused by artresia of tertiary follicles and structural changes of target-organs for ovarian hormones. The article has validated the hypothesis about absence of cerebrospinal fluid immunogenetic disparity of different mammal species and hypothesis about its contraceptive effect on derived samples during the donor’s physiological anovulation. We preserved normal characteristics of cerebrospinal fluid by intravital derivation, cryoconservation and we did not do filtration. Thus, we studied injected fluid as biological media and the biogenic medicine.