INTERNATIONAL JOURNAL OF LIFE SCIENCES

1-8

Authors: Khati Varada V

Moringa oleifera seed pod husk Activated Carbon was utilized as an adsorbent to remove water hardness ions from hard water. The effect of pH, adsorbent dosage, initial concentrations, contact time, and temperature were investigated using batch adsorption experiments. Characterization of adsorbent was identified by FTIR and XRD techniques. The pH dependence study of the adsorption process revealed that maximum pH for hardness removal was 8 with efficiency of 59%. Temperature study reveals that the adsorption is endothermic as efficiency increases with the increase in temperature. The adsorption of hardness ions on Moringa oleifera seed pod husk activated carbon shows that the removal efficiency increases with increase in contact time and also increased as adsorbent dosage increases from 1gm/50ml to 5gm/50m. The study showed that the method is a simple and efficient one to remove calcium and magnesium hardness from hard water solutions and adsorbent had the potential for hard water softening.

17-22

Authors: Niley Sanjay N, Kariya Kiran P and Berad Baliram N

The terpolymer resin has been synthesized by the condensation of 2, 6-dihydroxyacetophenone, ethylenediamine and formaldehyde in the presence of 2M HCl as catalyst with 1:1:2 molar ratio of reacting mono-mers. Composition of terpolymer resin was determined on the basis of elemental analysis. The number average molecular weight of the terpoly-mer was determined by conductometric titration in non aqueous medium. Viscosity measurements were carried out to ascertain the characteristics functions and constants of terpolymer resin. The terpolymer resin was further characterized by UV-Visible absorption spectra, IR and NMR spectra. Thermal degradation curve has been discussed in order to determine its mode of decomposition, order of reaction, activation energy, frequency factor, entropy change, free energy change and apparent entropy change. Kinetic parameters were calculated by using Freeman-Carroll and Sharp-Wentworth methods. The data obtained from Freeman-Carroll method was used to determine various thermodynamic parameters. Synthesized terpolymer shows antibacterial activity.

29-34

Authors: Maskey Sudhirkumar M

In the pesticide especially chlorpyrifos is currently one of the most widely applied pesticides in the all over the India. In this article, the copolymer RPHF-I using for effective adsorbents of chlorpyrifos pesticide from wastewater. Chlorpyrifos (CPS) pesticide may appear as pollutants in water sources, having undesirable impacts to human health because of their toxicity, carcinogenicity and bioaccumulation to tissue. The aim of the present study is an attempt to synthesize new group of copolymer RPHF-I with higher efficiency of chlorpyrifos removal from aqueous solution. In batch method experiment is carried out to remove the chlorpyrifos. The adsorption capacity of the copolymer was studied as the function of solution pH, concentration of chlorpyrifos and contact time of adsorption. The adsorptive applicability of copolymer was tested by Langmuir isotherm and Freundlich isotherm. The adsorption capacities were found to be 92.23% for pesticide removal. The copolymer RPHF-I can be successfully used as an efficient material for removal of pesticide from aqueous environments and can have a variety of potential environmental pollution control.

39-43

Authors: Nagpure Atul S

Octahedral Molecular Sieves (OMS-2) material was prepared by redox method and was employed as catalyst support for the preparation of heterogeneous OMS-2 supported metal catalysts. All the materials were systematically characterized using various techniques such as X-ray diffraction (XRD), N2 adsorption-desorption, Inductively Coupled Plasma-Optical Emission Spectrometry (ICP-OES), Transmission Electron Microscopy (TEM), etc. Highly dispersed supported metal nanoparticles were synthesized using ion-exchanged method. Characterization studies confirmed that metal particles are homogeneously distributed over OMS-2 support.

49-54

Authors: Hunge Sudhir, Rahangdale Pralhad Lanjewar Mamata

Environment is deteriorating day by day due to industrial pollution, toxic chemicals leads to the accumulation of heavy metals contamination in the waste water. In view of their toxicity, non-biodegradability and persistent nature their removal becomes an absolute necessity. Hexavalent chromium metal is one of the carcinogenic pollutant in the environment and is frequently present in wastewater from various industrial units. The present research article reports the characterization and use of chitosan-coated activated carbon derived from the bark of Pongamia pinnata (CCPPAC) as a potential adsorbent after for removal of hexavalent chromium from aqueous solution. SEM analysis proved the mesoporous nature of the material under investigation. The batch experiment was carried out to study the effect of significant process parameters such as pH, contact time, adsorbent doses and initial Cr(VI) concentration. The maximum adsorption efficacy for Cr(VI) removal by CCPPAC was found at pH 4.5, 5 gm/lit of adsorbent dose and 140 min contact time. Under optimum condition, 96% Cr(VI) was removed from aqueous solution. This investigation verifies that CCPPAC, a mesoporous material can be successfully used as an excellent sorbent material for removal of hexavalent chromium from contaminated water and thus can be applied in wastewater treatment.

58-62

Authors: Dharamkar RR and Meshram YK

A Simple and Economic Synthesis of 5-((benzylidene amino)methyl)-4-(substituted phenyl)-6-methyl, 3,4-dihydropyrimidin-2(1H)-ones by using 5-(aminomethyl)-4-(substituted phenyl)- 6-methyl- 3,4-dihydropyrimidin -2(1H)-ones and benzaldehyde in presence of ethanol. When banzaldehyde is react with primary amine i.e. 5-(aminomethyl)-4-(substituted phenyl)- 6-methyl- 3,4-dihydropyrimidin-2(1H)-ones in presence of alcoholic medium it gives formation of corresponding Schiff bases. The newly synthesized compounds were well characterized by IR, 1H NMR and mass spectral studies.

68-72

Authors: Siddiqui Naqui J, Mohammad Idrees and Manisha Rohankar

A series of 1,3,4-Oxadiazoles bearing Coumarine moiety (5a-c) were synthesized by refluxing Schiff’s bases (4a-c) with acetic anhydride. 4a-c needed for the synthesis was obtained by refluxing ethyl-2-oxo-2H-chromone-3-carbohydrazides (3a-c) with benzyloxy benzaldehyde. Similarly, 3a-c were prepared by reacting hydrazine hydrate and ethyl-2-oxo-2H-chromene-3-carboxylates (2a-c), which in turn were synthesized by treatment of substituted 2-hydroxy benzaldehydes (1a-c) with diethyl malonoate. The structures of the newly synthesized 1,3,4-Oxadiazoles have been established on the basis of chemical transformations, elemental analysis, IR, 1H NMR, and Mass spectral studies. The title compounds were screened in-vitro for antibacterial activity against two Gram positive and two Gram negative bacterial strains such as E. coli, S. aureus, B. thurengienesis and E. aerogenes. The zone of inhibition measured in mm revealed that the title compounds exhibited moderate to good antibacterial activity against Chloramphenicol as standard.

81-85

Authors: Gudadhe Swapnil K

Human being uses the earth’s bounty in profusion. Energy resources are in verge of extinction due to its over use. Conservation of traditional energy sources and harvesting renewable energy resources like solar energy, wind, biomass and sea-waves can bring out a substantial solution to the problem of energy crisis. To study the same two prime activities undertaken by Nagpur Municipal Corporation were taken into consideration for the study. Many campaigns along with Poornima Diwas Campaign were studied for the project and analysed the amount of energy resources saved through these activities. Along with them, it observed that an aggressive propaganda for harvesting solar energy and marketing several gadgets using solar energy are being systematically done by NMC to bring out a desired effect. The paper ends with the positive note that energy is to be conserved for brighter future.

89-94

Authors: Katkar Prafulla and Patankar Sanjeev

Enzymatic bioremediation of insecticides is receiving considerable attention, particularly since the extensive characterization of enzymes capable of detoxifying a range of organophosphate compounds. The basis of investigations for enzyme capable of detoxifying other classes of insecticides requires a source of enzyme for catalytic detoxification. This study describes the enrichment of a culture of soil bacteria capable of degrading endosulfan. The enrichment was achieved and maintained by providing endosulfan as the only carbon source. Endosulfan is poor biological energy source, as it contains only six potential reducing electrons and previous attempts to enrich for endosulfan degrading microorganism using the insecticide as sulfur source have been not very successful. However, endosulfan has relatively reactive cyclic sulfite diester group70. In this study, microorganisms were selected for their ability to release the carbon group from endosulfan and to use this as source of carbon for the growth. This selection procedure enriches for a culture capable of either the direct hydrolysis of endosulfan or the oxidation of the insecticide followed by its hydrolysis. In particular, enzymatic insecticide bioremediation is the focus of extensive study after the isolation of enzymes capable of detoxifying a range of organophosphate compounds from several bacterial species. An essential step in the investigation of an enzymatic method for endosulfan degradation is the definitive identification of a biological source of endosulfan degrading activity.

101-106

Authors: Pawar AL

Lonar Lake is a saline ecosystem formed entirely on basalt rock. It is a completely closed system a favors the growth of alkalophiles and halophiles. The Acenatobactor play a major role for conserving environment by utilizing toxic C1 carbon compound and reduces the pollution produced by methanol, green houses gases etc. In present study, methylotrophic bacterial isolates were isolated from water and sediment of Lonar Lake using minimal salt medium containing 2% (v/v) methanol as sole carbon and energy source. One bacterial strains were isolated and characterized morphologically, biochemically and identified as Acenatobactor by 16S rRNA sequencing. These Acenatobactor strains were further screened for its ability to utilize methanol by Spectrophotometric method. Results showed that the isolates ALP3 of Acenatobactor strains are found to be efficient methanol utilizer and could be effectively use for bioremediation by reducing methanol at polluted sites and also helpful in reducing C1 compound, and global warming gases from environment.

110-112

Authors: Khobragade Kavita D and Thool Vaishali U

In the background of managing staphylococcal infections, health departments are facing a slow down to curb the antibiotic menace. STX pigment promotes resistance to reactive oxygen species and host neutrophil-based killing and is a hallmark phenotype in virulence and is absent in the mutant or less virulent counterpart. Hence the aim of this study was to study the antibacterial effects of essential oils (EOs) against STX producing Staphylococcus aureus isolates from various clinical samples. Comparative activities of seven different essential oils were recorded. Thyme EOwas found to be most effective against most of the S.aureus isolates. All the S.aureus isolates (n=25) exhibit sensitivity to all the essential oils, however clove oil and thyme oil showed excellent antibacterial activity. The use of essential oils may prevent the development of infections and will minimize antibiotic use, prevent development of resistance as well as promote healing.

123-134

Authors: Margonwar AS and Kalkar SA

Melisssopalynological analyses are useful in enhancement of bee keeping industries. Pollen analysis of the nectar sources of Apis dorsata and Apis florea during summer honey samples from forest area of Gadchiroli district was carried out. 50 samples were collected during summer 2005 to 2009. Pollen grains were identified from reference slides, 18 samples were unifloral and 32 were multifloral. A total 72 pollen types were classified and they were representing 29 families. In the study major pollen types were recorded viz. Ageratum conyzoides, Syzygium cumin, Eucalyptus globulus, Terminalia arjuna, Tridax procumbens, Pongamia pinnata etc. The data reflect the floral situation of the place where particular honey was produced and identification of geographical origin based on the presence of combination of pollen types of the particular area and this area is suitable for establishing apiary industries.

143-147

Authors: Mungole Arvind*, Kharwade Priyanka and Wadekar Mohan

In the present study the protocol for callus induction and regeneration in Cassia absus was standardized. Young apical leaves, cotyledonary leaves, epicotyle and hypocotyle were used as explants for callus induction on Ms Medium containing IBA and kinetin in different concentrations also used BAP and kinetin in different concentrations. The maximum percentage of callusing was observed on the medium supplemented with 0.8mg/L IBA and 0.1mg/L Kinetin was found to be 100% for cotyledonary leaf & 90% for epicotyle explants. The calli in most of the cultures were brown and soft in nature. Initiation of shooting of Cassia absus established from cotyledonary leaf explants on MS medium supplemented with combination of hormones BAP 0.2 mg/L & Kinetin 0.6 mg/L. This study was aimed to develop standard protocol for callus induction, protocol for organogenesis & standardization of media and growth hormonal concentrations which may helps in conservation and cultivation of this species.

153-156

Authors: Gaikwad Deepmala A and Korpenwar Anil N

Spilanthes acmella (L.) Murr. Flowers are used as a raw material for the preparation of some important drugs for curing various human diseases. During unscientific methods of storage causing fungal contamination. The fungal contamination effect on the chemical composition of raw materials and thereby decreases potency of drugs. Regarding the above fact the present experiment was conducted and observed that the young flowers were showed no incidence of fungi on Blotter Paper Method except Aspergillus flavus on PDA. In case of mature flowers the Blotter Paper Method showed incidence of four fungi like Alternaria alternata, A. niger, Aspergillus terreus, F. oxysporum, Fusarium roseum whereas PDA Method showed nine fungi. The maximum incidences of fungi were reported in stored flowers as compared to young and mature flowers.

161-164

Authors: Khirade PD and Dudhe SS

In present study, dry seeds of Linum usitatissimum L. (linseed) were irradiated with gamma rays at different doses levels of 10 kR, 20 kR, 40 kR, 60 kR, 80 kR and 100 kR to examine its effects on growth traits and morphological variation. The experiments was carried out in two sets, one with treated seeds and the other untreated which served as control. Effects of this irradiation were evaluated by studying seed germination, seedling growth, leaf size, plant height and length of internode. Gradual reduction in seed germination was observed from lower doses to higher doses. Shoots were measured longer than roots at every dose and control. Shoot and root immerged from irradiated seeds showed gradual decrease in length except 40 kR where, shoots were measured shorter than roots. Biometric measurement of leaf length showed variation among various doses whereas leaf width is constant at all doses, at 100 kR it was moderately increased. Height of the plant showed variable effect in length. Length of internode was noted almost uniform.

169-172

Authors: Nasare PN

The present study was carried out on Phytoplankton diversity of Gaurala Lake in Bhadrawati, dist. - Chandrapur, Maharashtra state, India during 2015-2016. The present paper reveals the phytoplankton diversity in Gaurala Lake. During this study, 40 genera of phytoplanktons were recorded, out of 40 genera, 12 genera recorded for cyanophyceae, 23 genera for chlorophyceae and 5 genera for bacillariophyceae amongst three family members, chlorophyceae members found dominant and then cyanophyceae & bacillariophyceae. In case of chlorophyceae members, amongs them Vaucheria, Cosmarium, Spirogyra, Volvox, Chara and Oedogonium were found to be dominant. In case of cyanophyceae members, amongst them Nostoc, Anabaena, Oscillatoria, Anacystist, Microsystis were found to be dominant. In case of bacillariophyceae, Diatoms was found to be dominant. This indicates that the plenty of phytoplanktons are available in the lake and maintaining the ecological balance of the particular lake and will be helpful for the feeding zooplanktons and fishes which will maintain food chain and sustainable ecological balance of the lake.

177-180

Authors: Tiple Neha Ramesh and Dakhane Vimal P

Soil is a natural body of mineral & organic material differentiated into horizons, which differ among themselves in their morphology, Physical, Chemical & biological Characteristics. Plants depend on the soils for their nutrients, water & mineral supply. The soil type is a major factor for determining what types of plants will grow in any area. The physico-Chemical study of soil is based on various parameters like pH, Electrical Conductivity (EC), total organic Carbon (OC), Available Nitrogen (N), Available Phosphorous (P2O5), Available Potassium (K2O), Exchangable Calcium & Magnesium. Three soil samples were collected from three sites of farmland for soil analysis i.e. Sembal Village, Marda Village, and Ekarjuna village which are located near warora. Physical and chemical analysis of soil have done by different methods. Estimation of total Nitrogen (Kjeldahl method), Available Phosphorous (Bray’s & Olsen’s method), Available potassium (Flame photometric method) & exchangable Calcium & Magnesium (EDTA titration method). Fe, Mn, Zn, Cu determined by DTPA method. The results Shows that, samples taken have various parameters like EC, pH, OC, N, P, K, Ca, Mg, and Fe, Mn, Zn, Cu. The results depend on quality of soil samples.

186-188

Authors: Nirpane Sachin and Pandhure Narayan B

Kurdu i.e.Celosia argentea L. is a herbaceous plant belongs to family Amaranthaceous. Traditionally the plant material is used for the treatment of jaundice, gonorrhea, wounds and fever. The leaves are also used for the treatment of inflammations, fever and itching. The seeds are bitter, useful in blood diseases, mouth sores. They are efficacious remedy in diarrhea. Hence,in vitro techniques for multiplication of Celosia argentea was developed using apical buds and shoot tip as an explant.

192-194

Authors: Theng PA, Korpenwar AN and Borkar SU

Natural products from medicinal plants, either as pure compounds or as standardized extracts, provide unlimited opportunities for new drug. The present investigation conducted to produce Fourier transform‐infrared (FT-IR) and ultraviolet-visible (UV-VIS) spectrum profile of Geodorum densiflorum. Fourier transform‐infrared (FT-IR) and ultraviolet-visible (UV-VIS) spectrum profile of leaves extract in chloroform was used for evaluation. FTIR evaluation was used to detect the characteristic peak values and their functional groups. FTIR evaluation of leaves powder extract in chloroform revealed the presence of Hydrogen bonded alcohols, phenols, Alkanes, Aliphatic esters, Alkenes, Nitro compounds, Bromides and Aromatics groups. UV-VIS profile of leaves powder extract in chloroform showed the peaks at wavelength 426, 491, 536, 611 and 671 with the absorption 2.04, 2.078, 1.67, 1.37 and 2.18 respectively.

198-200

Authors: Matte RS and Kale MC

Antidesma ghaesembilla commonly known as Jondhurli it grows gregariously on open grasslands and scattered in mixed forest. Plantation can be raised both on irrigated and dry lands. Root suckers are freely produced and help in vegetative propagation. The plant is traditionally reported to possess astringent, bitter, alterative, aphrodisiac, anthelmintic, antibacterial and anti-asthmatic properties. As per phytochemical investigation, the ether, methanol and aqueous extract used for testing various chemical compound.

204-206

Authors: Watakhere Devilal N and Kale MC

Saoli region is basically an agriculture zone where majority of people survive on rice farming. In this area agricultural field is surrounded by the barbed wire fencing instead of natural fencing by hedge plants. There are several hedge plants which includes medicinal, ecological and wild fruit and vegetable plants. The present investigation focuses on the current status of hedge plants due to habitat destruction by modern agricultural techniques. The outcome of this research will open the debate on classical farming versus modern farming and also make awareness among the people about importance of hedge plants in nature.

210-211

Authors: Shende Mukund B and Deshmukh Umakant B

During floristic survey an interesting plant of family Malavaceae was collected from Sonegaon village of Chimur Tahsil , Chandrapur District of Maharshtra state. After critical observation it was identified as Abutilon theophrasti Medik. It is reported as a new distributional plant record for Chandrapur district. A brief description with photograph, notes on occurrence and distribution of this taxon are provided for easy identification.

215-225

Authors: Bansod Deepak S, Chavhan Pankaj R and Dhamani Amir A

It was aimed to study the size, weight and shape differences between right and left adrenal glands, the cortico-medullary ratio of the adrenal glands in bat Taphozous kachhensis. The specimen of Taphozous kachhensis were collected from Ambai Nimbai,45 kilometers from Bramhapuri (M.S.). Many collections were made during the breeding season so as to coincide with the time of reproductive cycle. These bats are very sluggish in nature after collection they were sexed and were brought to the laboratory. Weight recorded with digital balance before they were sacrificed. The Adrenal were dissect out and fixed in alcoholic Bouin’s fluid. After fixation for 24 hr tissue were washed with 70% ethanol. For the histological examinations, after fixation, tissue samples were dehydrated, cleared, and embedded in paraffin. Haematoxylin and eosin staining method was used to examine tissue sections. The size and weight of the gland were different from male to female bats. The adrenal gland is composed of two distinct cell layers, the cortex and the medulla. The medulla is composed of chromaffin cells that produce the hormones epinephrine and nor-epinephrine. The mean diameter and the weight of the Right and left adrenal gland is varies during different phases of the reproductive cycle. The cortico-medullary ratio in male was 65.57% cortex and 34.43% medulla (1.9:1) and in female 58.84% cortex and 41.16% medulla (1.43:1). Medullary tissue of adrenal glands was more in female whereas the cortical tissue was more in male and this suggests that the adrenal medulla hormones production was more in female bat and similarly, cortical hormone secretion was more in male bat.

235-239

Authors: Sayyad Nagma and Dhamani Amir

Catfish investigation of Pranhita River sub basin at Sironcha Dist. Gadchiroli, Maharashtra was carried out during July 2015 to June 2017. Pranhita River is boon for peoples of Sironcha. The documented paper deals with the diversity, abundance and conservation (IUCN) status of catfish. Sampling sites were selected along 10km and visited fortnightly. A total of 15 species of order siluriformes belonging to 5 families and 9 genera were recorded during study period. In study Bagridae was dominant family with 8 species, from families like Sisoridae and Pangasiidae single species recorded from each. Abundance shows majority of catfish are common, few are uncommon and only one species was rare. 4 catfish species were under near threatened category while 11 were under least concern category according to IUCN red list status.

247-253

Authors: Borkar KR and Deshmukh GD

Zooplanktons play a very crucial role in the trophic dynamics and energy transfer in aquatic ecosystem. Their abundance increases in eutrophic water. They are also sensitive to pollution and many species are recognized as indicators of pollution. It is an integral component of an aquatic ecosystem. The study site Janala Lake is located near Mul, situated between 200,07,N and 790 ,67, E. Water samples were collected once in month from the selected sampling sites of Janala lake to analyze the diversity of zooplanktons for the period of 24 months i.e. from January 2011 to December 2012. Zooplankton belonged to Rotifera, cladocera, copepod and ostracoda and both the lakes, the two years average showed the following sequence of their abundance. Janala Lake = Rotifera > Copepoda > Cladocera > Ostracoda. In the present investigation, total zooplankton was recorded maximum during summer and minimum during monsoon.

259-262

Authors: Gubbawar Shruti D

Insect social life is generally associated with increased exposure to pathogens and the risk of diseases transmission, due to factor such as high population density, frequent physical contact and reduced genetic variability. Honey bees are attacked by numerous parasite and pathogens towards which they present a variety of individual and group level defense behaviors that reduce colony level parasite and disease loads are termed social immunity. Large-scale losses of honey bee colonies represent a poorly understood problem of global importance. Both biotic and abiotic factors are involved in this phenomenon that is often associated with high loads of parasites and pathogens. A stronger impact of pathogens in honey bees exposed to neonicotinoid insecticides has been reported, but the causal link between insecticide exposure and the possible immune alteration of honey bees remains elusive. Here, we demonstrate that the neonicotinoid insecticide clothianidin negatively modulates NF-κB immune signaling in insects. Viruses and other pathogens can spread rapidly in social insect colonies from close contacts among nestmates, food sharing and periods of confinement. There is a relationship between the effectiveness of social and individual immunity and the nutritional state of the colony. Parasitic Varroa mites undermine the relationship because they reduce nutrient levels, suppress individual immune function and transmit viruses. The maintenance of the immune system can be costly, and a lack of dietary protein can increase the susceptibility of organisms to disease. All species of honey bees has shown great adaptive potential, as it is found almost everywhere in the world and in highly diverse climates. the precise impact of potential environmental changes on honey bees as a result of climate change, there is a large body of data at our disposal indicating that environmental changes have a direct influence on honey bee development.

267-270

Authors: Jha AK

In this study possibilities of the application of optimum conditions of the adsorption and coagulation treatment with bentonites was explored and optimized and effects of the treatment were focused on, water contaminated with dispersions of different organic polymers, water contaminated with food particles and waste water characterized by emulgated and fine dispersed solids. The turbidity and SS of the treated wastewater were reduced with the increase of bentonite dosage, and reached minimum at the same dosage which was about 250mg/L. The pH 6 as the optimum value was under consideration not only the treated efficiency but also the cost of the treatment.

275-278

Authors: Harney NV, Dhamani AA and Andrew RJ

Cladocera is a primarily-freshwater monophyletic group, an important component of the microcrustacean zooplankton. They inhabit most types of continental fresh and saline water habitats, occurring more abundantly in both temporary and permanent stagnant waters. The present paper describes the biodiversity of Cladocera fauna of Kanhala, Pindavani and Malhara pond, located near the Bhadrawati town of Chandrapur district. Qualitative and quantitative analysis of Cladocera community was undertaken on monthly basis from October 2005 to September 2007. A total of 12, 8 and 10cladocera species were identified during the period of Oct. 2005 to Sep. 2006, while a total of 10, 11 and 9cladoceraspecies were identified from Oct. 2006 to Sep 2007 in Kanhala, Pindavani and Malhara pond respectively. In the present investigation, Cladocera was maximum during the winter season and minimum during the monsoon season in all the ponds.

283-285

Authors: Rudey RJ

Hardy-Weinberg states that in random mating population an equilibrium is established between allelic frequency and remains unaltered from one generation to the next regardless of their dominant and recessive relationship if mutation, migration, selection, genetic drift does not act on it. In present study total sample of 289 individuals, aging from 18 to 79 years were surveyed from Nagbhid area. Those were the students of the college, their parents, relatives and other people from Nagbhid area to study the morphological trait of length of index finger in order to discourse Hardy- Weinberg law. As this is sex influenced character, males and females were analyzed separately to see whether it follows Hardy-Weinberg Principle or not. In the sample of 119 males, 80 showed dominant character and 39 males were recessive. In the sample of 170 females 136 were found recessive. And it was observed that both the sexes show very high degree of deviation than expected by application of Chi- Square test. Hence it was established that certain forces like nonrandom mating, mutation migration selection have great effect.