This review discussed the current research done on the most popular natural antifertility agent of plants origin and examines the weight of evidence to support the use of any of these substances to enhance antifertility activity and function. A variety of natural contraceptive of plants origin are known to have a potential effect on the sexual functions, supporting older claims and offering new hopes. The available synthetic drugs and treatments have limited efficacy, unpleasant side effects and contraindications in certain disease conditions. The present review, describes the detail information about the major constituents and their medicinal importance found in naturally occurring plants, which are helpful to further development of pharmaceutical formulations. In the present investigation total 6 plants were examine for their antifertility activity in female albino rats as claimed by tribal of Yavatmal and Amravati districts.
The extracellular proteases was purified by halophilic Bacillus sp. which having potential to grow at high salt conditions. The obtained protease has wide industrial applications mainly in food industry, detergent, medical and biotechnology. The extracted crude protease was purified by using ammonium sulphate, dialysis and ion exchange chromatography. The 80% ammonium sulphate gives the up to 2.4 fold purification of protease. The dialyzed sample was introduced on anionic exchanger as DEAE cellulose having a strong affinity towards the protease and gives 4.2 fold purification.
Biofertilizers are living cells that enrich the soil quality. They are eco-friendly and play an important role in the production of crop. Biofertilizer are cost effective as compared to chemical fertilizers and are also renewable source of energy. They maintain the long term fertility of the soil. Hence to increase the producity of the soil the use of biofertilizer is a must. The comparative effect of biofertilizer azotobacter spp on the growth and yield of Mungbean vigna radiata (L ,Wilczek) was studied. The seeds of mungbean were treated with biofertilizer and their result was recorded after 45 days. The results proved that plant grown with biofertilizer Azotobacter spp showed excellent results in the morphological and biochemical parameters. Hence the use of biofertilizers should be encouraged.
In the present investigation, production of (poly-β-hydroxy-butyrate) PHB by using Bacillus megaterium and Pseudomonas spp. was studied. The maximum PHB was produced by Bacillus megaterium than Pseudomonas spp. having 5.39% yield and 4.66% respectively. The different carbon and nitrogen sources were optimized for PHB production. Higher PHB accumulation was observed in the media containing sucrose as a carbon source and L-Lysine as a nitrogen source by Bacillus megaterium, whereas in Pseudomonas spp. higher PHB accumulation was in presence of sucrose as a carbon source and potassium nitrate as a nitrogen source. The PHB produced in the medium was extracted by using solvent extraction (chloroform) method and separated by TLC method.
Fourty one fungal species were found associated with seeds of some cereals. Maximum fungi were reported in Jowar, Maze, Bajra,wheat and paddy seeds. Aspergillus flavus and Curvularia pallescens were found to be more pathogenic and cause more deterioration due to production of amylase. Amylase can convert starch in to glucose and glucose is easily absorbed by fungi and seeds become more viable and showing high percentage of germination inhibition. Maximum loss in protein and starch was recorded at 35 C and 45 C due to C.pallescens and A. flavus respectively. Bavistin and dithane Z-78 were found more effective than other fungicides.
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