Biotechnologia Acta

7-13

Authors: F. Can, P. M. Pelin, A. Karahan, Z. Mustafaeva, B. Mamedov

The aim of the review was to emphasize the importance of producing new generation high standardized synthetic peptide Zika vaccines which induce both humoral and cellular immunity and eliminate side effects of traditional vaccines. The information was done about Zika virus that is an artropod-born virus, member of the genus Flavivirus in the family Flaviviridae. Zika virus has caused outbreaks in many countries the conditions in adults such as Guillain–Barre syndrome by the dramatically increasing number of cases. According to announcement by World Health Organization, 4 million people could be infected with Zika virus in Americas. The importance of the development of peptide vaccines against the virus Zika of the new generation, which are the most promising direction of the prevention and treatment of viral infection has emphasized.

19-27

Authors: N. O. Pushkarova, M. S. Kalista, M. A. Kharkhota, D. B. Rakhmetov, M. V. Kuchuk

The aim of the study was to establish efficient protocols of seed surface sterilization with further multiplication in vitro for threatened species Crambe koktebelica (Junge) N. Busch and to show the effect of biotechnological approach (in vitro cultivation) of biodiversity conservation on plants biochemical properties. Seed surface sterilization was carried out according to the original method with further microclonal multiplication of aseptic sprouts from lateral buds on the Murashige and Skoog (MS) medium supplemented with different concentrations of growth regulators. Fatty acid content was determined using Gas chromatography-mass spectrophotometry of fatty acid ethers. Antioxidant activity was determined using 2.2-diphenyl-1-picrylhydrazyl radical scavenging method. Total soluble protein content was measured using Bradford method and polyfructan content determination was based upon ketosugars ability to color in the acidic environment with resorcinol. Plants that were grown under in vitro and in vivo conditions and seeds were used in this research. Efficient protocol of surface sterilization that resulted in 45% of aseptic seed material 50% of which has sprouted was elaborated for C. koktebelica as well as fast microclonal multiplication methods that provided with up to 5.25 ± 0.50 new formed plantlets from 1 lateral bud (on the MS medium that contained 1 mg/L of 6-benzylaminopurine). It was also shown that aseptic cultivation benefits to saturated fatty acid accumulation and increases protein content but on the other hand it reduces unsaturated fatty acid amount and polyfructan content as well as antioxidant activity of plant material. Obtained data confirms the prospect of biotechnology approach to biodiversity conservation and suggest the necessity of father in vitro cultivation effect on biochemical composition of plant study.

35-41

Authors: T. Y. Trokhymchuk, E. N. Chumak, L. N. Moysa, V.A.Shevchuk, L. A. Ganov, V. G. Serdyuk, N.Ya. Spivak

The aim of the research was to determine the shelf life of the ELISA test kit DIA-HIVAg/Ab (PJSC "SPC" Diaproph-Med") intended for the determination of antibodies to HIV1/2 and p24 HIV1 antigen using accelerated storage model at elevated temperatures. It is established that the thermal inactivation process is subject to a first-order kinetic law. The dependence of the rate constants of inactivation (lnK) on temperature (1 / T) is described by the Arrhenius equation at 95% probability level (F-test). Calculated on the basis of this model, the activation energy (ΔEa) equals 23.27 kcal • mol-1. It is established that the projected shelf life of the test kit was 2 years and 1 month when stored at 4 °C in terms of reduction of its diagnostic activity by 10%. Isothermal method of accelerated storage based on the Arrhenius model can significantly save time by determining the expiration date of the test kit as early as at the stages of its development or modification. The obtained data can be used for confirmation of the diagnostic kit stability studies, in terms of long-term storage, correction recommended conditions, and for determination of test kit capability of withstanding exposure to adverse environmental factors, which may occur during transportation and storage.

50-57

Authors: H. M. Svitina, O. А. Rybachuk, L.V. Garmanchuk, V. A. Shablii, G. S. Lobyntseva

The aim of the research was to assess the impact of rat placental multipotent stem cells on the levels of proliferation and apoptosis in organotypic coculture with slices of colon tumors. Placental multipotent stem cells were isolated by explant method, colon cancer was modeled by administering 20 mg/kg dimethylhydrazine weekly for 20 weeks, tumors of the colon were cut into 500 µm slices. There were no changes in proliferative activity of tumor tissue, or in the level of apoptosis. Absence of influence of placental multipotent stem cells on colon tumor growth is a unique feature of these cells, and extends the application of placental multipotent stem cells in therapy.

67-76

Authors: L. V. Gorbunov, Ye. G. Cherniavska

The purpose of the research was to determine the regularities of influence of mammalian embryos heterogeneity and effectiveness of cryoconservation steps on their viability by using the developed simulation model. The model is based on analytical expressions that reflect the main causes of embryonic mortality during in vitro and in vivo cultivation, crioconservation and embryo transplantation. Reduction of viability depends on a set of biological factors such as the animal special, donor and recipient state, quality of embryos, and of technological ones such as the efficiency of cryopreservation method, and embryo transplantation. Fulfilled computer experiment showed, that divergence of embryos viability depending on biological parameters variations changes in a range from 0 to 100%, whereas efficiency index of chosen technology has an inaccuracy about 1%. The comparative analysis of alternative technologies of embryos cryopreservation showed the maximum efficiency of stages of use of the cryoprotectant, freezing regime and in vitro and in vivo cultivation of biological object. The application of computer modeling gives an opportunity to reduce the range of embryos viability results, obtained in different experiments is many times, thereby to shorten the time, monetary costs and the slaughter of laboratory animals in obtaining reliable results.