Biotechnologia Acta

18-23

Authors: L. A. Kolomiets-Babenko, O. S. Bohorad-Kobelska, N. L. Kovalchuk, M. Ja. Spivak, A. I. Kornelyuk

The goal of the research was to determine the ability of new nanocomposite preparation EMAP II (endothelial monocyte activating poplypeptide II) to affect the expression of the tumor-necrosis factor and interferon in vitro. In the experiments, the transformed cell line L929 cells was used. The induced interferon levels were determined in samples of culture medium by the microtitration method in the L929 cell culture against test virus vesicular stomatitus VSV. Toxicity of the substance was assessed by its maximum tolerated dose. The amount of endotoxins in nanocomposite preparation EMAP II was measured using gel-clot test. The range of concentrations of EMAP II causing the production of tumor necrosis factor was determined. The concentration of lipopolysaccharides in the tested nanocomposite preparation was less then 0.5 IEU/kg. New nanocomposite preparation EMAP II has the ability to induce TNF-α production at rather low concentration 1.56–25.00 μg/ml (82.49–1370.00 mol х 10–12). The interferon production under the influence of nanocomposite preparation EMAP II was not found. The results support the application of the target nanocomposite reparation EMAP II for cancer treatment.

30-37

Authors: N. Y. Yavorska, O. V. Lobachevska, Ya. D. Khorkavtsіv, N. Ya Kyyak

The aim of the work was to determine optimal conditions for clonal reproduction, growth and development of different varieties of Vaccinium corymbosum from the foreign selection. The objects of research were 5 varieties of highbush blueberry: Bluejay, of early ripening period; Bluecrop, Bluegold, Legacy, of average ripening; Aurora, of late ripening. Optimal conditions of explant surface sterilization have been selected depending on their type and effectiveness of used disinfectants. Maximum quantity of viable sterile regenerates was obtained using the mixture of sterilizing solutions of alcohol and bleach "Belizna" with Tween. Effectiveness of microclonal reproduction depending on the composition of nutrient agar medium with organic compounds on macro- and microsalt basis WPM (Woody Plant Medium) with addition of the growth-regulator of cytokinine action 6-γ, γ-Dimethylallylaminopurine (2іР) was evaluated according to regenerative activity of explants and shoots reproduction coefficient. The results of experiments showed that effectiveness of phytohormones action and their concentrations depended on genotype properties of every variety of V. corymbosum. It has been established that the growth activity and shoots multiplications changed depending on the concentration in nutrient medium 2iP. Maximum values of reproduction coefficient for Bluecrop and Bluegold varieties and shoots height were determined on the medium with 4 mg/l 2iP. For Aurora and Legacy the regenerative ability was the highest with concentration 8 mg/l 2iP and for Bluejay, 10 mg/l 2iP.

45-53

Authors: V. V. Klochko, S. D. Zagorodnya, O. N Reva

The aim of this research was to elucidate the role of fenesin-1-carboxylic acid of Pseudomonas batumici and diversity of the genes encoding its synthesis in bacteria of the genus Pseudomonas. Phenazine-1-carboxylic acid in the concentration of 10 μg/ml stimulated the biofilm formation by batumin-producing strain. The presence of the corresponding gene in the genome of P. batumici was not successfully confirmed by PCR amplification with a set of primers designed for Pseudomonas. The complete genome sequencing of P. batumici has revealed a homologous gene that could encode synthesis of this compound. Comparative study of sequenced Pseudomonas genomes showed presence of at least two genetically diverse groups of phenаzine coding orthologous genes. These genes could have distributed among rhizobacteria by the horizontal gene transfer.

65-69

Authors: S. Yu. Pokhylko, 2, V. V. Schwartau, L. M. Mykhalska, O. M. Dugan, B. V. Morgun

The aim of work was the analysis of effect of the gene Gpc-B1, introgressed from wild spelt Triticum turgidum ssp. dicoccoides, on the presence of biologically important elements in wheat winter bread hybrid families of generations F4 and F5. The accumulation of metals in ripe and unripe grains was measured on a mass spectrometer with inductively coupled plasma ICP-MS Agilent 7700x. It was found that the expressing gene significantly increased the content of trace elements Fe, Mn, Zn and Cu in ripe wheat kernels on average by 50‒70%, while the increase of Mg, Ca defined by an average of 20‒40%. Minerals enrichment confirmed during grain development and ripening provided not only biofortification for the future harvest, but also potentially enhanced the resistance of plants to diseases and formation of seedlings with more efficient use of nitrogen.