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INFLUENCE OF DIFFERENT TYPES OF TREATMENT ON THE OPTICAL PROPERTIES OF MUSCLE TISSUE OF ANIMAL ORIGIN
Authors: L.V. Plotnikova, A.P. Nechiporenko, S.M. Orehova, M.V. Uspenskaya, P.P. Plotnikov, A.L. Ishevskiy
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A comparative study of the effect of successive aqueous, salt and alkaline extractions on the spectral characteristics of the surface of ground pork muscle tissue and its main components was per-formed using attenuated total reflectance IR spectroscopy (ATR - IR) and electron spectroscopy of diffuse reflectance (ESDR). It is shown that, unlike IR spectroscopy, which is a method of group analysis of functional groups, the ESDR method makes it possible to test the component composition of complex biomaterials – proteins, lipids, carbohydrates. The high resolution, sensitivity and informativity of the method are determined by the discreteness of electron excitation energies of oxygen lone pairs present in carbonyl groups. These carbonyl groups are found in almost all materials of biologic tissue components, although belonging to their different types – sarcoplasm, muscle fibre as well as stroma and its proteins.