Current Research in Pharmaceutical Sciences

82-85

Authors: Harisharan Goswami, Bushra Malik and Meenakshi Chaturvedi

According to the WHO about 80% population of the world depend on medicinal plants for remedies. Medicinal plants are exploited for therapeutic medicine and have been used by Ayurveda, Unani and Siddha. In Ayurveda, Charak and Sushruta described various plants along with their medicinal values. Portulaca grandiflora, is a medicinal ornamental plant in the family Portulacaceae, Portulaca has many medicinal properties. It is used for the treatment of cirrhosis of the liver, pharyngeal pain and swelling. Leaves used for scurvy. Fresh juice of leaves and stems applied to snake and insect bites, burns, scalds, and eczema. In the present study micropropagation of Portulaca grandiflora was done using nodal explants, inoculated on MS medium containing kinetin (1.0-4.0mg/l). Axillary bud proliferation was observed within 05 days. After 3 weeks regenerated shoots shown fresh growth with 3-4 new nodes on each concentration of kinetin supplemented Murashige & Skoog medium. No callus initiation was observed on any of the Kinetin supplemented media. Out of the four concentration 2.0 mg/l Kinetin shown maximum percentage of Axillary bud proliferation i.e. 60%, whereas higher concentration of Kinetin shown stunted growth. Regenerated shoots were transferred into Murashige & Skoog media supplemented with 1.0- 4.0mg/Indole Butyric Acid (IBA) for root initiation. Root initiation was observed after 5 days of subculturing. Maximum percentage of root initiation and plantlet regeneration were recorded in 2.0mg/l IBA i.e. 90%, whereas minimum percentage observed on 4.0mg/l i.e 40%. Regenerated Plantlets were transferred to green house and finally to the field.

55-62

Authors: N. Vijaykumar

Immunoprotective activity of Septilin evaluated against Cyclosporine A induced immunosuppression in wistar albino rats. An equivalent of 100mg of Septilin tablet extract administered as an oral dose and its Immunoprotective activity assessed by determining of host resistance against E.coli, determining hematological parameters (Total Leukocyte Count), determining in vivo phagocytic test by carbon clearance, determining humoral response (by measuring antibody titre) and cell mediated immunity (by measuring foot pad thickness). The present study suggests that Septilin significantly protected the animals by increasing the immunoprotective activity in immune compromised conditions. In Septilin treated, immunosuppressed rats, there was 33.3% mortality against 100% mortality in immunosuppressed rats after 48hrs of infection. 16.6% mortality was observed in Septilin treated rats against 66.6% in immunosuppressed rats after 24hrs of infection. Furthermore, Septilin treatment raised the TLC significantly (P<0.01) when compared with control immunosuppressed rats. It was also found that Septilin treatment potentiated the phagocytic activity in immunosuppressed animals. The antibody titre determination study suggested that Septilin enhances both primary and secondary responses in rats immunized with BSA. In normal rats, Septilin increased the primary antibody titre values by 2.4 times and the secondary antibody titre values by 1.56 times. In immune suppressed rats, Septilin increased the primary antibody titre values by 2.5 times and secondary antibody titre values by 1.81 times. Septilin also showed the protective activity against impaired DTH conditions.

68-73

Authors: Neelkant Prasad, Roshan Issarani, Badri Prakash Nagori and Amar Deep Singh

This article describes the development of UV spectrophotometric method for detection of glibenclamide during its dissolution in alkaline borate buffer pH 9.5 because no official UV spectrophotometric method is described for this drug and no such method has been developed for estimating the drug in this medium. A stock solution of 20 g/ml was prepared. Various aliquots of the stock solution were transferred to a series of 10 ml capacity volumetric flasks so as to prepare various dilutions between the ranges of 2-12 g/ml. To each dilution 0.1 ml of concentrated hydrochloric acid was added and a calibration curve was formed at 229.5 nm between absorbance on Y- axis and concentration on X- axis. The linearity range was found to be between 2.0 to 12.0 g/ml. The limit of detection (LOD) and limit of quantification (LOQ) were found to be 0.23 g/ml and 0.71 g/ml respectively. The repeatability, interday and intraday precision (measured in terms of percent relative standard deviation, %RSD) was always less than 0.94%. The accuracy of the developed method were measured by recovery studies and was found to be between 100.39%±1.03% and 102.94%±1.46%. Thus, the developed method is specific, accurate and precise as validated according to recommendations by ICH guidelines.

27-30

Authors: Chandan B Jha, Nidhi Jain and A K Pathak

Radiopharmaceuticals have been majorly used in ages for the diagnostic agent and therapeutic agents .Recent years enormous efforts have been made to formulate holistic formulation for the therapeutic effect of Radiopharmaceuticals .Due to its versatile applications in cancer , Theraspheres -which are glass beads microparticals containing yttrium 90 isotope as a therapeutic agents for Osteosarcoma and its liposomes containing yttrium 90 for the treatment of throat and neck cancer have been formulated .These new formulations have added a new dimension in the field of Research and Development .This review explains need of Radiopharmaceuticals ,their advantages over conventional drug delivery systems and there different application in pharmaceutical fields.

45-49

Authors: Priyanka Parmar, Ashwani Mishra and Anupam Pathak

The present study investigates the use of ethosomal formulation of clotrimazole by mechanical dispersion method. In this work initially ethosomes of clotrimazole were prepared by using different concentration of soya lecithin and then optimized formulation batch was selected by determining Size and surface morphology , drug entrapment efficiency (DEE),in vitro release study and the best formulation was used to prepare gel by using carbopol 934 as a gelling agent . pH and Viscosity were determined of Ethosom entrapped gel. The formulated ethosomal gel obtained was exploited to achieve a zero order release profile of Clotrimazole. DEE was obtained between 52.6 to 60% in best formulations This study disclosed that RE5∗ was the best promising formulation among all prepared formulations.

1-5

Authors: Bibekananda Meher and Anupama Roy

Nowadays obesity is a major health problem all over the worlds especially in developed and developing countries. A person with a BMI equal to or more than 25 is considered overweight. Excess body weight is a result of an imbalance between energy intake and energy expenditure. Obesity is responsible for a number of health complications like diabetic and cardiovascular. Drugs available in market posses a number of adverse effects. Leptin is adipose derived cytokine which is essential for regulation of body weight and energy balance. Leptin regulate lipid profile and glucose metabolism, neuroendocrine functions. Leptin exerts immediate effects by acting on the brain to regulate appetite. Leptin is the first identified adipose-tissue-derived cytokine, which exerts profound functions in the regulation of food intake, energy expenditure, glucose metabolism, reproduction and immune response.

16-20

Authors: Munesh Mani and Devendra S Rathore

Ailanthus excelsa (Family: Simaroubaceae) is commonly known as Mahanimba and Arlu. In this study stem bark of Ailanthus excelsa were evaluated by anti-implantation and abortifacient method at three doses i.e. 100mg/Kg, 150mg/Kg and 200mg/Kg of the body weight of the rat. It was observed that treatment with methanolic extract of Ailanthus excelsa at the dose of 200 mg/kg was shown significant anti-fertility effect. Ailanthus excelsa possess anti-fertility activity by inhibiting implantation and abortifacient action.