International Journal of Applied Sciences and Biotechnology

1-7

Authors: Ndirika D. Nnaebue, Chikodili G. Anaukwu, Victoria N. Anyaoha, Chukwuma O. Soludo, Amarachukwu B. Isiaka, Tobechukwu M.C. Ajogwu, Samuel C. Onuorah

Bryophyllum pinnatum is a plant abundant in several phytochemicals, that can be extracted, purified, and packaged for the goal of promoting public health. In this study, the phytochemical composition of the leaf extracts of Bryophyllum pinnatum grown in Anambra State, Nigeria, was compared. B. pinnatum leaves were obtained from a farm in Anambra State, Nigeria. The leaves were processed and extracted using cold water and hot water extraction, methanol extraction, and ethanol extraction. The extraction processes were carried out following standard extraction methods. Preliminary qualitative and quantitative analyses were conducted to determine the presence and concentration of phytochemicals in the plants. Phytochemicals tested include alkaloids, flavonoids, saponins, tannins, anthocyanins, reducing sugars, carbohydrates, proteins, cardiac glycosides, terpenoids, and phlobatannins. All the tested phytochemicals were recovered in the solvent (methanol and ethanol) extracts; carbohydrates, reducing sugar, protein, and anthocyanin were absent in the aqueous (cold and hot water) extracts. Varying concentrations of the phytochemicals in the different extracts were recorded, and they range from 0.00±0.00% to 13.30±0.22%, with the highest concentration of the extracts recovered in the solvent (methanol and ethanol) extracts. This study revealed that the extraction method used can influence the type and concentration of phytochemicals recovered in Bryophyllum pinnatum. Therefore, the specific desired phytochemical should determine the extract method to use in any study.

19-28

Authors: Prateek Joshi, Sanjeev Kumar Karn, Pramod Koirala

This article explores the intricate landscape of stakeholder analysis and management within the framework of promoting small and medium-scale vegetable processing technology in Nepal. Given that agriculture engages a substantial 66% of the country's workforce, the escalating demand for vegetables has been met with challenges stemming from the lack of support for vegetable processing industries, resulting in food waste and financial setbacks for farmers. Addressing these issues, the article advocates for holistic backing and promotion of vegetable processing industries in Nepal. The methodology section delineates a mixed-methods approach for stakeholder analysis, employing documentary reviews, key informant interviews, and surveys to get information. It further explores the constitutional roles of different government levels and details the initiatives by the Ministry of Agriculture and Livestock Development (MoALD) and other government agencies in propelling food processing technology. The stakeholder analysis provides a detailed examination of both internal and external stakeholders, categorizing them based on their power and interest. The power/interest grid and influence mapping assist in prioritizing stakeholder engagement and managing their expectations effectively. The article concludes by asserting that effective stakeholder management is crucial for addressing the challenges and promoting the growth of vegetable processing industries in Nepal.

39-45

Authors: Jamuna Bhattarai, Angira Kumari Chaudhary, Surya Koirala, Ruby Shah, Sabina Adhikari, Nidhi Kamal Kushwaha

Background: Community assessment is the basic foundation for promoting and improving the health of community populations. The objective of this survey is to appraise the health profile of the community and assist in promoting community health. Methodology: A descriptive cross-sectional survey was carried out from August 14, 2019, to September 21, 2019. The population of the study was residents of ward number 1, Chhapkaiya of Birgunj Metropolitan City, a Slum area of the ward selected purposively, where there were 200 houses and a total population was 1364. Data were collected using the interview technique. Descriptive statistics were used to analyse data. Results: The majority (79.0%) of respondents threw waste in an open field. Only 19.0% of respondents had used latrines. Most women (80.25%) became mothers at the age of fewer than 20 years. More than half (57.31%) of pregnant women had done an ANC visit up to the fourth time, among them, 90.0% of respondents had delivered in a government hospital 86.0% of the mothers had colostrum feeding practiced as well and 90.12% had done exclusive breastfeeding up to 6 months. Female sterilisation (38%) were more common in the society. Above sixty percent (62.86%) of families, had done diarrheal management in the home of under 5 children. Conclusion: The health profile of the Chhapkaiya community is satisfactory. All populations used safe water but open field defecation is prevalent and most of the population threw waste in the open field. The majority of the women became mothers below 20 years of age and home delivery is still practiced in this community.

171-180

Authors: Shreejana Pandey, Guheswari Chataut, Suja Maharjan, Dev Raj Maidali, Keshab Bhattarai

Jimbu (Allium przewalskianum), is an indigenous Himalayan spice herb in Nepal and has a long history of culinary use, yet its potential beyond home kitchens remains largely unexplored due to limited studies and exposure. It does not require extensive input of cash, machinery, land, or labor to profit from, making it an excellent and valuable source of pharmaceutical ingredients owing to its bioactive compounds. We investigated its antioxidant and antimicrobial properties. Quantitative analysis of Jimbu extracts revealed the presence of bioactive compounds, including Oleoresin (extracted using acetone and methanol), polyphenols, flavonoids, and tannins. The methanol extract of. A. przewalskianum exhibited the highest polyphenol content (8.11±0.24 mg GAE/g), flavonoid content (5.28±0.29 mg GAE/g) and tannin content (0.923±0.02 mg GAE/g). The antioxidant potential of Jimbu was seen to be increased with an increase in concentration, reaching 58.79% at 400µg/ml, with an IC50 value of 303.58µg/ml for DPPH radical scavenging activity. Likewise, the yield of oleoresin was obtained as (10.86±0.27) from acetone and (7.8±0.20) from methanol. Similarly, sensory evaluation through One-way ANOVA highlighted concentrations of 0.07% and 0.1% of Oleoresin to be most favored. Furthermore, sensory evaluation of the Jimbu extracts in minced chicken meat exhibited remarkable antimicrobial activity against Salmonella along with a significant reduction in the colony forming units (CFU). This study shows that the extracts of A. przewalskianum could be utilized as a potential medicinal plant for the development of effective drugs against pathogenic bacteria. Meanwhile, it provides opportunities for economic benefits and a new potential revenue source for rural communities in the Himalayan region of Nepal.

186-196

Authors: Nikita Mahat, Niharika Bhattarai, Milan Thapa, Mousam Lawati, Anup Basnet

In Nepal, traditional medicinal plants and herbs have long been relied upon for treating various diseases. This study was aimed to explore the antimicrobial properties of commonly used traditional medicinal plants. This study was conducted from December 2022 to June 2023 at the Department of Microbiology, St. Xavier's College, Maitighar, Kathmandu. In this study, antimicrobial property of 12 plants were tested against four ATCC cultures of bacteria- Staphylococcus aureus (ATCC 6538P), Staphylococcus epidermidis (ATCC 12228), Escherichia coli (ATCC 8739) and Pseudomonas aeruginosa (ATCC 9027). The extracts were prepared using Soxhlet's apparatus with ethanol and distilled water as solvents. The ethanolic extract of S. aromaticum had the highest percentage yield The ethanolic extract of O. sanctum showed highest potential against S. aureus with a zone of inhibition of 21mm. The ethanolic extract of S. aromaticum demonstrated antimicrobial activity against S. aureus, E. coli, P. aeruginosa, and S. epidermidis, with respective zones of inhibition of 18mm, 16mm, 18mm, and 20mm. The distilled water extract of A. marmelos displayed the highest antimicrobial activity against E. coli, with a zone of inhibition of 18mm. Phytochemical screening revealed that both extracts of S. aromaticum had the highest presence of phytochemicals. The ethanol extract of Z. armatum exhibited the highest antibacterial activity, with a minimum inhibitory concentration (MIC) value of 2mg/ml against S. aureus. The study of the selected plants extracts confirmed their efficacy as natural antimicrobials against the tested organisms, suggesting their potential for novel drug development in treating infectious diseases.

209-216

Authors: Deepak T.A. , Avinash Tejasvi M.L

Systemic Health goes together with oral health. In India, Oral health remains a big challenge. It is connected tightly to overall individual’s health. Basic oral hygiene such as brushing teeth twice a day is still foreign to most, while such practices are widely practiced in the west. In India, economic factors, coupled with normally accepted behavior and acceptance of poor oral hygiene, prevention of awareness contributed to vast populace with dental issues, like cavities, gum diseases, premature teeth loss, and oral cancers in some cases. With almost 60% of 1.4 billion living in rural areas, initiatives like oral hygiene awareness, preventative care, lifestyle changes and increased dental facilities including universities, colleges and dental care facilities are needed today. We investigate whether there is an association between awareness of teeth alignment, good oral health to overall systemic health. We developed a questionnaire for dental patients falling between the age of 13 - 21 years. This range was selected as most of the patients are old enough to know their oral habits, and other health issues. The patient’s data was collected over a range of 8 months (April 2023 to November 2023) between two states in southern India. The research had 191 patients who were from rural areas, and their awareness on oral hygiene activities were found to be far worse than their urban counterparts (both genders). Based on the data collected, we establish a close relationship between awareness of teeth alignment, to gum bleeding, caries, and pollinosis in young Indians.

123-127

Authors: Ouation Souleymane Soro, Vincent Kadjo, Koffi Sylvanus Aubert Yao, Moussa Kimse

The aim of this work is to test the effect of green forage Ipomoea batatas on young rabbit (Oryctolagus cuniculus) digestive health and growing performance. Two group (T and I) of 15 rabbits were made. These animals were weaned at 35 days of age and slaughtered at 70 days. The mean weight per group was 500 ± 20 g. Control group T was feed only with industrial pelleted Ivograin®. Experimental group I was feed with the same pelleted diet and green forage I. batatas in the cage. Results showed that the growing performances in group I were lower than control. Group I daily gain was 0.89g lower than T (P<0.01). Fed intakes was 1.05 times lower with forage than control (P<0.01). For sanitary risk index (mortality + morbidity) was 2 part higher in group I than control (14 vs 7, P<0.01). However, the nutrient digestibility was higher with I. batatas group than control. Digestibility of DM, OM, CP, NDF, ADF and Hc was 26.68, 23.43, 9.67, 18.56, 18.74 and 16.46 respectively higher with group I than control (P<0.05). We can conclude that, associate green forage I. batatas with pelleted diet was not increased the young rabbit growing performances and health except nutrients digestibility.

135-142

Authors: M. Paudel, B. Bohara, T. Prasai, S. Bhattarai, T.R. Gompo

The dairy sector in Nepal has a high potential for growth, but unfortunately, its progress is impeded by diseases such as mastitis. The purpose of the current investigation was to identify the predominant bacterial species in mastitis-affected cattle milk and to ascertain the most efficacious antibacterial treatment against the bacterial isolates. The study was conducted from 17th August 2022 to 12th February 2023 in lactating cattle of the Kirtipur municipality and Chandragiri municipality of Kathmandu district, Nepal. A total of 640 milk samples from 160 cattle were collected randomly from cattle farms of Kirtipur and Chandragiri municipality of Kathmandu district. Initial screening for mastitis was conducted on collection sites by the California Mastitis Test (CMT) and further bacterial isolation and identification were carried out at the Central Veterinary Laboratory using standard bacterial techniques and the antibiogram were evaluated based on CLSI guidelines. Among 640 samples collected from 160 cattle, 3.91% (25/640) tested CMT-positive at the individual quarter level, whereas the prevalence at the cow level was 15.62% (25/160). The major bacterial species isolated, in subsequent culture of CMT positive samples, were Staphylococcus spp. (10, 40%), E. coli (6, 24%), Streptococcus spp. (5, 20%), Enterococcus spp. (2, 8%), Bacillus spp. (1, 4%), and Klebsiella spp. (1, 4%).. Antibiogram study reveals that major gram-positive bacteria, Staphylococcus spp., Streptococcus spp., Enterococcus spp., and Bacillus spp. were highly sensitive to Ampicillin, Florfenicol, and Gentamicin while the major gram-negative bacteria, E. coli, and Klebsiella spp. were highly sensitive to Florfenicol, Gentamicin, Trimethoprim/Sulfamethoxazole and Ceftriaxone. Hence, early diagnosis and careful monitoring of antimicrobial susceptibility before therapeutic intervention are essential to prevent high economic losses due to mastitis and to mitigate the potential risk of antimicrobial resistance in the livestock population.

152-157

Authors: Ashok Sah, Prakash Gurung

The experiment was conducted in Ratuwamai Municipality, Morang, Nepal to assess the impact of various cropping systems on soil fertility and nutrient status. Four different cropping systems (Rice-Wheat-Rice, Rice-Mustard-Maize, Rice-Maize and Rice-Mustard-Rice) were selected as treatments and all treatments were replicated five times in Randomized Complete Block Design. Soil samples from 0-15 cm depth were collected from each site and evaluated for soil pH, Soil Organic Matter content (SOM), Total Nitrogen (N), available phosphorus (P), available potassium (K), sand silt and clay content. All the tested parameters except Soil N content, sand content and clay content were found to be significantly affected by the cropping system. The soil in all four cropping systems were found acidic in nature with pH ranging from (4.18-4.48). The Soil Organic Matter was recorded highest (1.84%) from Rice-Maize based system and lowest amount of Soil Organic Matter was observed in Rice-Mustard-Rice based system. The highest N content (0.19%) was recorded from the Rice-Mustard-Maize cropping system and the lowest N content (0.02%) was recorded from Rice-Wheat-Rice cropping system which is statistically similar to Rice-Mustard-Rice based cropping system. The highest P content (163.95kg/ha) was recorded from Rice-Mustard-Maize and the lowest (75.28kg/ha) was recorded from Rice-Mustard-Rice based cropping system. The highest K content (169.18kg/ha) was recorded from Rice-Mustard-Rice cropping system and the lowest K content (57.76kg/ha) was observed in Rice-Wheat-Rice based system. The highest silt content (48.38%) was recorded from Rice-Mustard-Maize cropping system. The result indicated that the Soil Organic Matter, Nitrogen and Potassium were found to be deficit nutrients in all the cropping system.

54-59

Authors: Shreesha Uprety

Genome-wide association studies (GWAS) test hundreds of thousands of genetic variants across many genomes to find those statistically associated with a specific trait or disease. A Comprehensive review of studies on biotic resistance, abiotic tolerance, yield-associated characteristics, and metabolic composition is provided. Genome-wide SNP maps have characterized population structure concerning the geographic origin and morphological type and identified patterns of ancient crop diffusion to diverse agroclimatic regions. To better understand the genomic patterns of crop diversification, nucleotide diversity variation, linkage disequilibrium, and recombination rates across the genome are quantified. Results from GWAS can be used for a range of applications. The GWAS approach has proven highly suitable for identifying common SNP-based variants with moderate to significant effects on phenotype. However, the genetic factors underlying some of these associations have been characterized. The vast majority remain unexplained. The development of next-generation sequencing and bioinformatics tools has dramatically improved and is currently being implemented to decipher the genetic diversity of targeted traits. The significant drawbacks are the need for large population size, the cost of preparing DNA samples, and less knowledge about the risk of the quality. To overcome this drawback, researchers have upgraded the statistical approaches, proper imputation of genotypes, and advanced approaches such as nested association mapping and candidate gene association mapping. The primary benefit is one-time genotyping and repeated phenotyping in different environmental conditions.

66-77

Authors: Sharmila Sigdel, Aruna Khanal, Subhash Kumar Thakur

The clinical impact of β-lactamase has become a public health problem around the world in terms of increased morbidity and mortality, especially in the child population. This study was aimed at determining the bacteriological profile and detection of β-lactamase producing bacteria isolated from the blood samples of neonates. For this study, a total of 1335 blood samples of neonates admitted in NICU, SCBU, and sepsis-suspected neonates visiting Paropakar Maternity and Women’s Hospital, Thapathali, Kathmandu, Nepal were collected and processed. Blood culture was performed and the identification of bacteria was done by following standard microbiological methods. Antibiotic susceptibility testing was done by using the Kirby Bauer Disk Diffusion method and confirmation of ESBL, MBL, and KPC-producing bacteria was done by Combined Disk Test. The prevalence rate of neonatal sepsis was found to be 17%. K. pneumoniae 116 (50.2%) was the predominant Gram-negative bacteria followed by K. oxytoca 31 (13.4%) whereas S. aureus 39 (16.9%) was the predominant Gram-positive bacteria causing neonatal sepsis. Among 182 Gram-negative bacterial isolates, 69 (37.9%), 22 (12.1%), and 14(31.1%) were found to be ESBL, MBL, and KPC producers respectively. K. oxytoca (54.8%), Enterobacter spp. (25%) and Citrobacter spp. (14.3%) were the predominant ESBL, MBL, and KPC producers respectively. The co-production of ESBL, MBL, and KPC was also found among the 5 Gram-negative bacteria. Colistin, Meropenem, and Imipenem seem to be the choice of the drug against Gram-negative bacteria, whereas Vancomycin and Cefoxitin seem to be the choice of the drug against Gram-positive bacteria. Therefore, to lessen the burden of antibiotic resistance, it is essential to conduct regular antimicrobial susceptibility surveillance, periodic reviews of hospital settings, and early detection of beta-lactamase-producing bacteria.

85-98

Authors: Garima Lo Tamang, Bijaya Adhikari, Mahesh Shrestha, Achut Ram Pradhananga, Bindra Devi Shakya, Dipesh Raj Pant, Ramesh Kaji Shakya, Jasana Maharjan, Sudarshana Shakya, Pawan Raj Shakya

In this study, concentrations of Cd, Cr, Mn, Pb, and Zn were determined by flame atomic absorption spectrophotometer (FAAS) in edible muscle tissues of a total of 25 fish samples belonging to five fish species viz., Hypophthalmichthys nobilis (Bighead), Wallago attu (Buhari), Catla catla (Catla), Clarias batrachus (Mugree), and Labeo rohita (Rohu) marketed in Kathmandu, Nepal and evaluated potential health risks for adults using USEPA deterministic approaches. The average metal accumulation in all fish species followed the order of Zn (27.89) > Mn (5.36) > Pb (2.37) > Cr (1.12) > Cd (0.44) mg/kg, exceeding the FAO/WHO guidelines except for Zn. Likewise, the sum of average metal concentrations (∑5HM) followed the descending order of C. catla (49.61) > C. batrachus (44.49) > W. attu (34.51)> H. nobilis (32.10) > L. rohita (25.18) mg/kg. The correlation matrix showed significant correlations among some HMs indicating their common sources of origin in the examined fish species. The estimated daily intakes (EDIs) of HMs were lower than the maximum tolerable daily intake (MTDI). The target hazard quotients (THQs) of a single element in all fish species were less than 1.0 while the total target hazard quotients (TTHQs) in C. batrachus, C. catla, and H. nobilis exceeded the safe limit of 1.0 suggesting a potential non-carcinogenic risk. Moreover, the target cancer risks (TRs) of Cd and Cr were higher than the acceptable risk limit (10−4) in all fish species suggesting that their consumption might pose a lifetime cancer risk for adults. Therefore, the study recommends regular monitoring of HMs in commercial fish to ensure the safety of consumer health.

106-115

Authors: Shishir Dahal, Bijaya Dangi, Raman Kumar Dangi, Prakash Bista, Ashmita Sharma Upadhyaya, Sunita Bharati, Sunil Dulal

Mandarin (Citrus reticulata) is the major sub-tropical fruit popularly grown in slopy terraces of mid-hills which has high domestic and foreign market potentialities. Scientific studies are hardly carried out to assess the socio-economic aspects of mandarin production in this area. So, this research was aimed to assess the production and profitability of mandarin production in Salyan district. For this, 61 respondents of Baghchaur municipality and 59 of Chhatreshwari rural municipality were selected using random sampling procedure which makes altogether 120 respondents in the study area. Pretested semi structured questionnaire were asked with farm decision makers for primary data collection. Collected data were encoded in MS excel version 2021, and required inferences on socio-economic and demographic characteristics, BC ratio, cobb-douglas production function and problem ranking were made by using SPSS version 26. Independent t test was used to compare the means between two municipalities. The results revealed that the annual mandarin production was 3560.58 Kgs per household with productivity of 14.60 tons per hectare. Average annual variable cost of production and revenue were NRs 227309.41 and NRs 988963.52 per hectare respectively. BC ratio was 5.07 indicating it as profitable business with the benefit of NRs 4.07 per rupee invested. Among the various determinants, cost of organic manure, human labor and plant protection materials had significantly increased while cost of irrigation had decreased the returns from the enterprise at different significance levels. The return of scale was 0.936 indicating decreasing rate of return of mandarin production.

1-7

Authors: Roman Pandey

Black shank, caused by Phytophthora nicotianae, and Fusarium wilt, caused by the Fusarium oxysporium f. sp. nicotianae, are major diseases affecting tobacco crops globally. Phytophthora nicotianae is primarily found in tropical and subtropical regions and infects tobacco plants by producing zoospores that swim to root tissue and form cysts. Symptoms of black shank include root and crown rot, wilting, leaf chlorosis, stem lesions, and pith necrosis. Management strategies for black shank include cultural practices, chemical treatments, and host resistance. Chemical fungicides, such as metalaxyl or mefenoxam, oxathiapiprolin, and fluopicolide, can be used but there is a risk of fungicide resistance. Fusarium wilt is characterized by yellowing, drying, and death of leaves, leading to the death of the entire plant. Control measures for Fusarium wilt include plant resistance, cleanliness, crop rotation, nutrition, nematode management, and fumigation or biofumigation. The most successful control of Fusarium wilt has been through the use of resistant tobacco cultivars. The impact of rotation crops and resistant plants on the pathogen populations in the soil must be evaluated and soil pH and calcium levels may also impact the disease. The presence of the fungus in a field can last for several years without a vulnerable host.

15-24

Authors: Manoj Kumar Shah, Luma Nidhi Pandey, Rupa Bastola, Bimala Shah, Shatrughan Shah

This study was commissioned to assess studies on feeding resources and feeding management of goat in mid hills of Nepal. During the study, 162 goat farms located in Ghiring and Baradi of Tanahun districts in Nepal were surveyed, and a semi-structured questionnaire was administered to each farm. Farmers in this region prefer integrating livestock raising with crop production. The majority of the farms surveyed kept indigenous Khari goats, either in pure flocks or through crossbreeding. The types of goat farming were commercial (8.02%), semi-commercial (29.01%) and subsistence (62.96%). The nature of management practices for goat farmers was intensive (70.99%), semi-intensive (14.81%) and free range (14.20%). Farmers grazed their livestock in different locations, including their own land (48.77%), forests (22.22%), and other places (29.01%). The selection of fodder species by farmers varied depending on the availability of different species in the mid hills, reflecting differences in their perception and preference. Among the top fodder species highly preferred by the farmer in summer season are Ficus cunia followed by Bauhinia longifolia, Ficus hispida, Castanopsis indica and Ficus auriculata. Fodder species highly preferred by the farmers in winter season are Litsea polyantha followed by Drepanostachyum khasianum, Shorea robusta, Terminalia elliptica and Mangifera indica. Goat production is an essential aspect of subsistence farming, providing both meat and cash income. Consequently, this study recommends promoting locally preferred tree fodder species and supplementing them with feed during different seasons, based on their availability and local preferences. Such strategies can help to improve goat production and support sustainable livelihoods for farmers in the region.

30-36

Authors: Ram P Mainali, Sunmoon Jyakhwa

Nepal possesses a rich diversity of cucumber landraces and their wild relatives. The first step towards harnessing the potential of these landraces in crop improvement programs is to identify the amount of genetic variation present among them. This research aimed to evaluate 18 cucumber accessions collected from different regions of Nepal and assess their agro-morphological variation in terms of yield and yield-associated traits under polyhouse condition. A research trial was conducted using Randomized Complete Block Design with two replicates at the National Agriculture Genetic Resources Centre (NAGRC), Khumaltar in 2019. The accessions were characterized using various qualitative and quantitative descriptors, with six focusing on traits related to the fruits of the plant and three on phenology and morphology. The results revealed significant variations among the genotypes for all studied traits. Bhaktapur Local was an excellent genotype for recording yield and associated traits, like fruit dimension, number of fruits per plant and yield per plant, making it the most promising genotype, however, Genebank accessions, such as NGRC08671, NGRC07716 and NGRC08668 could be excellent choices for yield associated traits. Correlation analysis indicated a positive relationship between yield and fruit length, fruit width, and total number of fruits per plant, while vine length was positively correlated with the total number of fruits produced per plant. This study identified several genotypes with desirable traits for yield and yield-associated characteristics, which could be valuable in the development of high-yielding cucumber varieties with superior morphological and agronomic traits in the future.

42-53

Authors: Hamadou Abdou, Ekengele Nga Léopold, Mahamat Seid Ali Malloum, Dieudonné Bitom

The main objective of this study was to assess the level of metal contamination of the soils used in the cultivation of onions in the city of Abéché. Thus, 04 plots were carried out and monitored for a period of five years (05 years). Two traditional plots were chosen in the Arkou site; and two control plots were carried out in the Bagarine site. Soil samples were taken annually (2016 to 2020) after harvesting on a thickness of 0 and 20 cm. In each plot, thirteen (13) samples were taken. After quartering, one (01) representative sample from each plot was retained. The samples were analyzed by optical emission spectrometry at the Food Quality Control Center Laboratory (FQCCL) in Ndjamena. The determination of heavy metal concentrations revealed relatively low levels for Ni, Zn, Cu, Cr and Fe. These levels are generally lower than the reference values ​​for all the plots. Geoaccumulation indices (Igeo) and Contamination factor (Cf) revealed pollution indices. Pb and Cd show contaminations in traditional plots. The correlations (P < 0.05) observed in our study area are positive and significant. This study shows that the use of chemical inputs are sources of soil contamination by heavy metals.