Hygeia journal for drugs and medicines

1-15

Authors: Balamani* & M. Sekar

Aspirinamino acid amides 1(a-f), Aspirin dipeptide amides 2(a-f). tripeptide amides 3(a-f) and tetrapeptide amides 4a and 4c were Synthesized. The synthesis of dipeptide amides were carried out in solution by stepwise elongation of the peptide chain from the C-terminal amino acid by coupling one amino acid at a time using DCC/HOBt method. The analgesic activity was studied by the acetic acid induced writhing test. From the mean writing values, it is evident that the entire sequence of the tetrapeptide amide namely, Gly-Gly-Phe-Met-NH2 is essential for the observed analgesic activity of compound A. And, the entire sequence of the tetrapeptide amide namely, Gly-Gly-Phe-Leu-NH2 is essential for the observed analgesic activity of compound C. The decrease in analgesic activity of these compounds is in the order, aspirin tripeptide amides > aspirin dipeptide amides > aspirin amino acid amides.

16-18

Authors: S. Mondal*, H. Ramana and P. Suresh.

The present study reports anthelmintic activity of various extracts of the bark of Neolamarckiacadamba (Roxb.) Bosser (Family: Rubiaceae) against Indian earthworms Pheritima posthuma. The results revealed that that all the tested extracts of N. cadamba bark possess anthelmintic activity in a dose dependant manner. The activities were comparable with the reference drug Piperazine citrate. Among the tested extracts, the chloroform extract and pet ether extract were found to possess promising anthelmintic activity in comparison to other extracts. The presentstudy, therefore, justifies its use in the folklore remedies as an anthelmintic drug of natural origin.

26-31

Authors: Abiramasundari.P, Priya.V, Jeyanthi.G.P, and Gayathri Devi. S*

Herbal products prepared either from single or multiple botanical ingredients are usually complex and variable in nature. Undoubtedly, the plant kingdom still holds many species of plants containing substances of medicinal value that have yet to be discovered. For this reason, Cocculushirsutus (L.) a medicinal plant belonging to family Menispermaceae, the perennial climber was selected for the present study. The study was formulated with the objective to assess the antibacterial activity of Cocculus hirsutus.The leaves stem and the root of Cocculus hirsutus was extracted with solvents namely petroleum ether, chloroform, benzene, acetone, methanol, and water for the determination of antibacterial activity. The chance to find antibacterial activity was more apparent in methanol and acetone extracts than the other extracts. The benzene and methanol extracts of Cocculus hirsutus leaves have shown strong antibacterial activity against all the organisms tested. The zone of inhibition ranged from 8-16 mm. The petroleum ether, acetone, and methanol extracts of Cocculus hirsutus stem have shown pronounced antibacterial activity against all the microorganisms tested. Zones diameter ranged from 9-21 mm. Based on the results, it can be concluded that the Cocculus hirsutus plant extracts have great potential as antimicrobial components against microorganisms and they can be used in the treatment of infectious diseases caused by resistant microorganisms.

38-47

Authors: M.Sekar*andJ. Balamani

Tetrapeptidederivatives of Aspirin (1 to 4) were synthesized by the following methods. Aspirin was prepared by acetylation of salicylic acid using Ac2O/ AcOH. Aspirin was coupled with amino acid amide and dipeptide amide using its p-nitro phenyl (NP) ester. The ester (Aspirin – ONP) was prepared by treating p-nitrophenol andDCC in EtOAc and was precipitated by using EtOH. The synthesis of dipeptide amides was carried out in solution by stepwise elongation of the peptide chain from the C-terminal amino acid by coupling one amino acid at a time usingDCC/HOBt method. Boc-group was used from the Nα protection of all amino acids. The Boc-group cleavage was carried out using 50% TFA / CH2Cl2.The amidation of C-terminal amino acid was carried out by treating the corresponding Boc – aminoacid – ONp esters with dry NH3 in presence of DCC and HOBt. Their gastric ulcer-inducing property was studied by histopathological method.

57-61

Authors: Thomas Kurian* and Jose Kurien

A simple, fast and precise simultaneous multicomponent derivative spectrophotometric method has been developed for simultaneous determination of Ampicillin and Probenecid pharmaceutical formulation. The first derivative spectra have absorption maxima at 222.2nm for Ampicillin and 288 nm for Probenecid in 0.1N NaOH used as a solvent. The peak with good absorption at different concentrations, obeyed BeerLambert law only was chosen. Both Ampicillin and Probenecid showed linearity in the range of 10 to 50 μg/ml concentration.