Hygeia journal for drugs and medicines

1-5

Authors: Ekbal Al-Khateeb, Ara Adkar Ohan*,Hakeem Al-Ani

Plan In the present study, the steroidal fraction was isolated from the ethanolic extract of T.communis root and berries (TCRE &TCBE, % yield: 0.035 and 0.032 respectively) and screened for their cytotoxicity against human epidermoid laryngocarcinoma (HEp-2) and Ahmed-Mohammad-Nahi-2003 mammary adenocarcinoma (AMN-3) cell lines. Methodology Tamus communis (F: Dioscoreaceae) is a plant, growing wildly in the northern part of Iraq, mainly contains the steroidal sapogenin, diosgenin, in its roots and berries. In the present study, the steroidal fraction was isolated from the ethanolic extract of T.communis root and berries (TCRE &TCBE, % yield: 0.035 and 0.032 respectively) and screened for their cytotoxicity against human epidermoid laryngocarcinoma (HEp-2) and Ahmed-Mohammad-Nahi-2003 mammary adenocarcinoma (AMN-3) cell lines. The presence of diosgenin was confirmed in the extract by HPLC methods with standard diosgenin sample. Outcome The plant extracts, TCRE and TCBE showed better cytotoxic effect (IC50 342.56 μg/ml and 386.55µg/ml) on HEp-2 cell line than pure diosgenin (IC50 409.29 μg/ml), while diosgenin was more toxic to AMN-3 cell lines (IC50 360.04μg/ml) compared to the plant extracts (TCRE: IC50 400.28 and TCBE: 465.64μg/ml respectively).

15-20

Authors: Rakesh Narang,Sunil Sharma, Balasubramanian Narasimhan*

Plan: Anti-inflammatory evaluation of acid hydrazide derivatives. Prologue: Hydrazide derivatives of organic compounds forms a significant group of derivatives which exhibit an arrayof biological activities like anti-inflammatory, antibacterial, anti fungal,anti-neoplastic, antiviral etc. In view of above facts, we hereby reported the anti-inflammatory evaluation of selected most active eight antimicrobial acid(nicotinic acid, naphthalene-1-acetic acid and 2-naphthoxy acetic acid)hydrazide derivatives. Methodology: The anti-inflammatory activity of reported hydrazide derivatives determined by carrageenan induced paw oedema method and diclofenac sodium as standard drug. Outcome: Anti-inflammatory activity showed that nicotinic acid hydrazide derivatives with NO2 substituents at ortho and meta position were the most active ones among the selected hydrazide derivatives, having 35.73% and 37.29%; 25.12% and 34.17%inhibition, at two different doses 20 mg/kg and 50 mg/kg b.w., respectively.

27-32

Authors: Narendra Nyola* and Govinda SamyJeyabalan

Plan:An analytical method for estimation of Azithromycinand Cefixime in bulk and tablet formulation. Methodology: A rapid, sensitive and specificuv-vis method was developed and validated for the estimation of Azithromycin andCefixime in tablet dosage form. The method was validated in terms of linearity,accuracy, precision, specificity, limit of detection and limit of quantitation.Theoptimum conditions for the analysis of the drug were established. The maximumwavelength (λ max) of Azithromycin andCefixime were found to be 235 nm and 288nm respectively. The percentagerecovery of Azithromycin and Cefixime were 100.28-100.33and 99.68-100.29 respectively. Beer’s law were obeyed in the concentrationrange of 10-50μg/ml for Azithromycin and 2-10µg/ml for Cefixime. The linear equation for AzithromycinandCefixime were found to be y = 0.0187x - 0.0143, r² =0.9996 and y = 0.0917x + 0.026, r² = 0.9985 respectively.Validation was performed as ICHguidelines for linearity, accuracy, precision, LOD and LOQ. Outcome: The proposed method wassuccessfully applied for the quantitative determination of Azithromycin and Cefixime in tablet dosage form.

41-48

Authors: L. Priyanka Dwarampudi , Dhanabal SP*, Shanmugam R , Muruganantham N

Plan: Traditionally in India and China, Psoralia corylifolia seed decoction used for treating psoriasis. However, the antipsoriatic activity of Psoralia corylifolia seeds has not been scientifically evaluated using mouse tail model. Methodology: Ethanolic extract of Psoralia corylifolia seeds were analyzed by high performance thin layer chromatography (HPTLC) .The In vivo antipsoriatic activity of 95% of ethanolic extract of Psoralia corylifolia seeds was done by using mouse tail model for psoriasis and In vitro antipsoriatic activity was carried out by Sulphorhodamine B (SRB)assay using HaCaT human keratinocyte cell lines. Outcome: HPTLCfingerprinting revealed the presence of gallic acid. The ethanolic extractshown IC50 255 μg/ml, with goodantiproliferant activity when compared with Asiaticoside as positive controlwith potent IC50 value of 20.13 μg/ml.Psoralia corylifolia seed extract showed an overall antipsoriatic activityof 75.87%, when compared with standard tazarot gel activity 87.94% by using mouse tail model, confirmed itstraditional use in psoriasis treatment.

57-64

Authors: Sripriya M, Rajendran P*, Seethalakshmi I, Kannan M, Ashok G, Karthikeyan M, Selvarani P

Plan To study the distribution of Hepatitis B and C viral markers amongthe weavers population from Kanchipuram, Erode and Tiruvannamalai Districts ofTamil Nadu. Methodology Medical camps were organized in three districts (Kanchipuram, Erodeand Tiruvannamalai) based on information about the weavers population who livein these localities. Three hundred and two blood samples were collected non-randomizedand screened for Hepatitis B surface antigen (HBsAg) and Anti HCV by ELISA. Outcome HBsAg positivity rate of Kanchipuram District was (3.09%), ErodeDistrict (2.04%) and Tiruvannamalai District (1.88%) HCV positivity was observedin one person only in Tiruvannamalai District (0.94%). There was no significantassociation between age, gender and the incidence of HBV and HCV positivity (p≥ 0.05 at 5% confidence).The percentage of HBV (HBsAg) and HCV in the weavercommunity is similar to the general population.

70-77

Authors: Nirmala Halligudi

Plan: To review on the pharmacological potential of flax seeds Prologue: Flax oil and flax seeds are being rediscovered as truehealth foods. They definitely merit being included on any top-ten list of foodsthat are good for life. Flax is not a new food. It is actually one of the olderand, perhaps, one of the original "health foods," treasured becauseof its healing properties throughout the Roman Empire. Flax was one of theoriginal "medicines" used by Hippocrates. Flax could be dubbed the"forgotten oil." It has fallen out of favor because oil manufacturershave found nutritious oils to be less profitable. Outcome: The very nutrients that give flax its nutritional benefits- essential fatty acids - also give it a short shelf life, making it moreexpensive to produce, transport, and store. Besides being the best source ofomega 3's, flax oil is a good source of omega 6, or linoleic acid (LA).Sunflower, safflower, and sesame oil are greater sources of omega 6 fatty acidsbut they don't contain any omega-3 fatty acids. Flax oil is 45 to 60 percentthe omega-3 fatty acid alphalinolenic acid (ALA). In addition to nutritiousfats, flax seeds contain other nutrients which make eating the whole seedsuperior to consuming just the extracted oil.

87-94

Authors: S. Lakshmi Devi* and Madhu.C. Divakar

Plan: The study was aimed to evaluate the wound healing activity of 70% ethanolic extract (WAET) of the leaves of Wrightia arborea (Apocynaceae) and the phytosome (WAP) prepared out from WAET using incision and excision wound models on wistar albino rats. Methodology: Wound contraction and epithelization period were assessed in excision wound model whereas wound tensile strength was determined in the case of incision wound model. Outcome: The Wrightia arborea phytosome (WAP 4%) exhibited significant wound healing potential when compared to WAET and standard 0.2% Nitrofurazone Ointment.