International Journal of Medical Laboratory

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Authors: Salvatore Chirumbolo *

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Authors: Hamed Faghihi, Abolghasem Abbasi Sarcheshme, Seyed Hassan Hejazian *

Volume 4, Number 1 (2017) | IJML 2017, 4(1): 56-62 | Back to browse issues page XML Print Download citation: BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks Send citation to: Mendeley Zotero RefWorks Faghihi H, Abbasi Sarcheshme A, Hejazian S H. The Relaxant Effect of Carvacrol on Acetylcholine-induced Contraction in Isolated Rat's Ileum . IJML. 2017; 4 (1) :56-62 URL: http://ijml.ssu.ac.ir/article-1-162-en.html The Relaxant Effect of Carvacrol on Acetylcholine-induced Contraction in Isolated Rat's Ileum Hamed Faghihi, Abolghasem Abbasi Sarcheshme, Seyed Hassan Hejazian * Abstract: (31 Views) Background and Aims: The antioxidant and anti-inflammatory effects of carvacrol were reported. The aim of the current study was an investigation the relaxant effects of carvacrol on acetylcholine-induced contraction in isolated rat's ileum. Materials and Methods: In this study, the tissues separated from ileum were fixed on the organ bath containing Tyrode's solution. The amplitude of contractions has been recorded using isotonic transducer. Before and after subjecting the tissues with saline and different concentrations of carvacrol,the procedure was elicited by cumulative logarithmic concentrations of acetylcholine (Ach). Results: Our results indicated that carvacrol at 10-2, 10-3 and 10-4, but not 10-5 M decreased acetylcholine (10-4 M)-induced contraction (10-2 M at p<0.001; 10-3 and 10-4 at p<0.05 vs. Ach+saline). The antispasmodic effect of carvacrol was examined and showed that in concentrations of 10-5, 10-4 and 10-3 M, but not 10-6 M, the contractive effect of acetylcholine10-3 M was prevented significantly. The concentrations of 1, 2 and 4 µM of verapamil were also tested and indicated that 4 µM of verapamil reduced contraction by 22.1±.5% (p<0.01 vs. saline). In samples treated with combined carvacrol (10-5 M) and verapamil (4 µM) contraction percentage was decreased by 35±14%, which was significantly different compared to the Ach+saline group as well as the single treated groups (p<0.01 and p<0.05, respectively). Conclusions: According to our findings, due to the effect of carvacrol and verapamil, the relaxant mechanism of carvacrol may be mediated by Ca+2 release from the sarcoplasmic reticulum and the response of the contractile system.

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Authors: Arsalan Salari, Fatemeh Moaddab, Mahsa Pourabdollah Tootkaboni, Maryam Shakiba, Mohadeseh Poursadeghi *

Background and Aims: Bilirubin has been considered an antioxidant that protects against atherosclerosis. The aim of this study was to evaluate the relationship of serum bilirubin level with the severity and complexity of coronary artery disease (CAD) and long-term outcome in the patients undergoing primary percutaneous coronary intervention (PCI). Materials and Methods: This prospective cohort study was performed on 136 patients with STEMI undergoing PCI in Heshmat Hospital, Rasht, Iran during 2 years. The complexity was calculated by using dedicated software of the syntax score and the severity of CAD was determined based on the number of vessel involvement. Also, long-term outcomes were evaluated by major adverse cardiac events (MACE ) after one year. Results: The mean total bilirubin level was 0.95 ± 0.69 mg/dl; 60.3% of the patients were in the low range of syntax score (0-22) and 38.2 % had two vessels involved. In examining the relationship between serum bilirubin level with the severity and complexity of CAD and MACE, There was no statistically significant association (p>0.05); but, the majority of the patients with high bilirubin levels had a lower number of vessels involved and the complexity of the vessels; Also, MACE was observed in the patients with low bilirubin levels. Conclusions: According to the results, attention to serum bilirubin level could be important in CAD and the protective effect of bilirubin can be used for new therapeutic decisions. Further studies on a larger sample of patients have been recommended to confirm and complete these findings.

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Authors: Yaser Eskandari Torbaghan, Mohammad Kazemi Arababadi, Ali Shams *

Background and Aims: Engagement of the receptor for advanced glycation end products (RAGE) and its ligand “S100A12 protein” induce a cascade of reactions that eventually might lead to develop an inflammatory response dependent on NF-κB. Although involvement of S100A12 and RAGE in some autoimmune disease have proved, in chronic hepatitis B (CHB) infection functions of the proteins are not clear thus far. Determining of expression of S100A12 and RAGE in peripheral blood cells of the CHB patients was the aim of the present study. Materials and Methods: In the case-control study the mRNA levels of S100A12 and RAGE genes of the sixty CHB patients and sixty healthy donors were measured by real-time polymerase chain reaction method. The patients and healthy donors were sex and age-matched. Results: The findings demonstrated expression of S100A12 and RAGE in the CHB patients significantly decreased compared to the healthy donor group (p=0.001). Expression levels of the genes were not altered among HBeAg-positive and HBeAg-negative CHB patients. The HBV-DNA copy number/ml did not affect the expression of S100A12 and RAGE in the patients (p>0.05). Conclusions: The results of the study suggested that down regulation of RAGE and S100A12, because of their role in inducing inflammation, might have a considerable role in chronicity of hepatitis B.