Polymorphism’s assessment of children’s candidate genes associated with low-level long-term exposure to strontium in drinking water
Authors: Zaitseva N.V. ,Dolgilh O.V. , Krivtsov A.V., Starkova K.G. , Luchnikova V.A., Bubnov O.A., Otavina E.A., Bezruchenko N.V., Vdovina N.A.
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A sequencing of the candidate genes of the pupils, exposed to strontium by the method of targeted resequencing has been performed. It is shown, that under conditions of increased revenues of strontium in drinking water the number of polymorphonuclear altered portions of candidate genes increases. As a result of the targeted resequencing in conditions of strontium exposure, the maximum polymorph modifications of the following genes are defined: sulfotransferase 1A1 (SULT1A1) and methylenetetrahydrofolate. It was shown that the structure of the mutations in conditions of the strontium exposure was characterized by the formation of defects in the gene mapping detoxification (38.5 % of all mutations) and immunoregulation (22.5 %). Analysis of the cause-effect relationships in the system "factor - the number of mutations" revealed that candidate genes reflecting strontium exposure conditions (content of strontium in drinking water is 1.3 MAC), are genes: cytochrome P450, glutathione - transaminase (detoxification); dopamine (CNS), interleukin 17 and the major histocompatibility complex (immune system), methylene-tetra-hydro-folate-reductase (reproduction).