Identification of essential and toxic elements in urine by icp-ms for diagnostic research and health risk assessment.
Authors: Ulanova T.S., Gileva O.V., Stenno E.V., Veykhman G.A., Nedoshitova A.V.
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The results of method developing for identifying 12 elements in urine are presented using a quadrupole mass spectrometer with inductively coupled plasma Agilent 7500cx (USA) with octopole reaction/collision cell (ORS) in the reaction mode. Urine samples were analyzed immediately after dilution in 1/10 of 1% aqueous solution of HNO 3 . The results’ validity is confirmed by standard analysis of urine samples SeronormTM urine (Norway). The developed method can accurately and reliably identify vanadium, chromium, manganese, nickel, copper, zinc, selenium, strontium, cadmium, lead and titanium in the urine samples. Limit of detection for V, Cr, Mn, Ni, Sr, Cd, Tl, Pb is 0.1 μg/dm 3 , for Cu 1.0 μg/dm 3 , for Se 5.0 μg/dm 3 , for Sr and Zn 50.0 μg/dm 3 with an identification error 25 %.
Biological substrates’ study of child population in the Perm Krai, living in ecologically safe conditions, performed by the developed method of mass spectrometry with inductively coupled plasma, allowed to identify background levels of 12 elements as comparison criteria for the problems of hygiene assessments, epidemiological studies, surveys, appraisals.