Asian Pacific Journal of Tropical Medicine

352-357

Authors: Zi-Li Gong, Chun-Mei Luo, Sheng-Zheng Wu, Hong Ran, Jie Zhu, Jian Zheng

Objective To investigate the protective effect of ultrasound-combined microbubbles on hippocampal acetylcholinesterase (AchE) fibers in rats. Methods According to random digits table, 60 SD rats were divided into two groups, marrow stromal cells (MSCs) intracranial transplantation group and MSCs intracranial transplantation + ultrasonic microbubbles group. Marrow stromal cells were cultivated and isolated in vitro; 12 weeks after transplantation, spatial learning and memorizing abilities of rats were assessed by Morris water maze; AchE staining method was used to observe changes in density and appearance of AchE staining positive fibers in hippocampal CA1 region. Results There was asignificant increase in spatial learning and memorizing abilities of rats in MSCs intracranial transplantation + ultrasonic microbubbles group. Hippocampal AchE staining suggested an increase in the density of AchE staining positive fibers in MSCs intracranial transplantation group; the fibers were regular, intact and dense. Density of hippocampal AchE positive fibers was negatively correlated with the escape latent period and was positively correlated with percentage of the time needed to cross each platform quadrant. Conclusions Better promotion of spatial learning and memorizing abilities of rats in MSCs intracranial transplantation + ultrasonic microbubbles group may be related with the protective effect of ultrasound-combined microbubbles on hippocampal acetylcholine fibers.

364-367

Authors: Qiao-Li Su, Shuang-Qing Li, Duo-Ning Wang, Feng Liu, Bo Yuan

Objective To study the influence of MicroRNA-10b on proliferation and invasion of human low metastatic lung cancer cell 95-C and its mechanism. Methods Lipofectamine MicroRNA-10b eukaryotic expression plasmid was transfected into 95-C. The experiment group was divided into blank control group, empty vector transfected group and MicroRNA-10b transfected group. Real time quantitative RT-PCR was used to detect the expression of MicroRNA-10b and KLF4mRNA expression. Proliferations of cells were detected by cell proliferation assay, invasion of the detected the cell Transwell experiments, the expression of KLF4 protein was detected in Western blotting cells. Results The proliferation rate of MicroRNA-10b plasmid transfection group increased significantly after transfection, invasion and migration ability enhancement, by comparison, there are statistically significant differences in the blank control group and negative control group (P<0.05); the expression of MicroRNA-10b plasmid transfection group KLF4 protein decreased, the difference was statistically significant (P<0.05); reduce the expression of MicroRNA-10b plasmid transfection group KLF4mRNA, but no significant differences (P>0.05). Conclusions MicroRNA-10b may promote proliferation and invasion of 95-C cells by down regulating the expression of KLF4 protein.

373-377

Authors: Xin-Peng Cong, Wen-Hui Wang, Xi Zhu, Can Jin, Liang Liu, Xin-Min Li

Objective To investigate the effect of stromal interaction molecule 1(STIM1) knockdown on the proliferation and migration of endothelial progenitor cells (EPCs) after vascular injury and its mechanism. Methods The rat bone marrow derived EPCs were divided into three groups: adenovirus negative control (group NSC), rat STIM1 adenovirus vector transfection group (group si/rSTIM1) and rat &human recombinant STIM1 adenovirus transfection group (group si/rSTIM1+hSTIM1). The STIM1 expressions in each group were detected by reverse transcription PCR after transfection; the cell proliferation was tested by [3H] thymidine incorporation assay (3H-TdR); Cell cycle was analyzed by flow cytometry; the cells' migration activity was detected by Boyden assay; Calcium ion concentration was detected by using laser confocal method. Results 48 h later after transfection, the expression level of STIM1 in si/rSTIM1 cells was significantly lower than that in NSC group (0.21±0.12 vs 1.01±0.01, P<0.05); EPCs that stayed in G1 phase in si/rSTIM1 group [(93.31±0.24)%] were significantly more than that in NSC group [(78.03±0.34)%, P<0.05]; EPCs' migration activity in si/rSTIM1 group (10.03±0.33) was significantly lower than that in NSC group: (32.11±0.54, P<0.05); EPCs calcium ion concentration changes in EPCs in si/rSTIM1 group (38.03±0.13) was significantly lower than that in NSC group (98.11±0.34, P<0.05). While there was no significant difference between si/rSTIM1+hSTIM1 group and NSC group on the four indexes above. Conclusions Silence of STIM1 attenuates EPCs proliferation and migration after vascular injury, by mediating the calcium ion concentration in EPCs.

382-385

Authors: Jie Zhang, Sheng-Guo Chen, Kaken Habaerxi, Shawuti Alimujiang, Yu Chen, Ming-Zhen Peng, Rong Yue, Yu-Lian Wu, De-Quan Wang, Yu-Ming Chen

Objective To observe reinforcing effect of calcium sulfate cement (CSC) bovine bone morphogenetic protein (bBMP) on vertebral in the rabbit model of osteoporosis. Methods A total of 48 New Zealand white rabbits were randomly divided into group I (blank control group), group II (CSC injection group), group III (CSC/bBMP injection group) and control group. White rabbit osteoporosis model was established rapidly by using castration method+methylprednisolone candidate. After modeling, groups II, III were given corresponding vertebral body injection material, and 4 animals were sacrificed respectively at 24 h, 6 weeks, 12 weeks after vertebral plasty. Tissue pathological status, vertebral mineral density and vertebral body bone mechanical strength were observed. Results Vertebral body structure form was normal in the groups II and III. Trabecular bone coarsens, connection and repair were observed in micro fracture and bone defects, bone trabecular connectivity was superior to group I significantly; vertebral body compression strength in the groupI was on the decline, vertebral compression strength in the groups IIand III was on the rise, the largest vertebra. Postoperative BMC and BMD in groups IIand III were incresed, and significantly higher than group I after 6 weeks (P<0.05), BMC and BMD in group III after 12 weeks were higher than the other three groups. Conclusion Compound bBMP CSC has good bone induction. It can improve the three-dimensional construction effect for osteoporosis vertebral trabecula, and can significantly improve the vertebral strength, as a vertebral packing material with good application prospect.

390-393

Authors: Jian-Lan Yu, Jun-Hua Li, Rong-Gui Chengz, Yan-Mei Ma, Xiao-Juan Wang, Jing-Chun Liu

Objective To observe the preventive and control effect of matrine on transforming growth factor (TGF-β1) and hepatocyte growth factor (HGF) of liver fibrosis tissue in rats. Methods A total of 48 SD rats were randomly divided into A, B, C, D groups with 12 in each, group A as the normal control group and groups B, C, D as liver fibrosis models using composite modulus method with carbon tetrachloride (CCL4). Group B was the model group, group C adopted γ-interferon lavage therapy in the second day of modeling, and group D adopted matrine lavage treatment, at 4 and 8 weeks after treatment. Six rats were executed for detection of TGF-β1 and HGF, liver tissue histology and comparison fibrosis degree changes of rat liver tissue between groups. Results Groups B, C, D showed a more significantly increased TGF-β1 at each time point compared with group A (P<0.05); Group B showed a more significantly increased TGF-β1 than groups C and D at weeks 4 and 8 (P<0.05); group D showed a lowest level of TGF-β1, followed by groups C and B. HGF of group B decreased more significantly than A group at weeks 4 and 8 (P<0.05); HGF of groups C and D was significantly elevated at 4 and 8 weeks than groups A and B (P<0.05), in which the group D showed the highest level of HGF. According to tissue histologic observation, rat liver tissue structure of group A was clear and normal, tissue structure of group B was destroyed with obvious fibrous tissue hyperplasia and fatty change of hepatic cells; groups C and D showed a slighter liver tissue damage, cell necrosis and connective tissue hyperplasia in collect abbacy than group B with a trend of obvious improvement. Conclusions Matrine can reduce TGF-β1 expression and enhance the activity of HGF, so as to realize the inhibition effect on liver fibrosis in rats.

398-401

Authors: Yan Xu, Yu Yang, Ying-Quan Luo

Objective To investigate the effect of atorvastatin on serum oxidative stress and N-terminal brain natriuretic peptide expression in rats. Methods A total of 40 healthy male SD rats were randomly divided into the sham group (Group A, n=10, saline 5 mL/d), ischemia-reperfusion group (Group B, n=10, saline 5 mL/d), atorvastatin group (Group C, n=10, atorvastatin 20 mg/kg•d), atorvastatin + N-amino-arginine group (Group D, n=10, atorvastatin 20 mg/kg•d + N-amino arginine 15 mg/kg). Myocardial ischemia-reperfusion rat model was established after 3 days of gavage. N-amino arginine 15 mg/kg was given by tail vein injection 15 min before ischemia. After reperfusion, enzymology indicators such us creatine kinase (CK) and lactate dehydrogenase and the oxidative stress parameters such as nitric oxide (NO), malondialdehyde (MDA) and total superoxide dismutase (TSOD), and n-terminal pro-brain natriuretic peptide (NT-proBNP) expression was detected by immunohistochemistry. Results LDH and CK levels of group A were significantly lower than the other three groups, and group B was the highest. There was significant difference between group B and group C (P<0.05), and no significant difference between group B and group D (P>0.05). MDA levels in group B were significantly higher than the other three groups. The lowest was group A, followed by group C, the difference among groups was significantly (P<0.05). TSOD and NO levels in group B was the lowest, the level in group A was the highest, followed by group C, the difference among groups was significant (P<0.05). NT-proBNP level in group B was significantly higher than the other three groups, the lowest was group A, followed by group C, the difference among groups was significant (P<0.05). Conclusions Atorvastatin has a protective effect on the myocardial injury in the myocardial ischemia and reperfusion rats. It can increase NO synthesis and decrease MDA content, increase serum TSOD activity and the oxidative stress effect, meanwhile protect myocardial cells and reduce myocardial injury.

407-411

Authors: Yan-Li Cao, Wei Zhang, Yan-Qun Ai, Wen-Xia Zhang, Yi Li

Objective To investigate the effects of propofol and ketamine on the cognitive function and immune function in young rats. Method A total of 80 young rats were randomly divided into four groups: Control group, ketamine group (experimental group A), propofol group (experimental group B), ketamine and propofol group (experimental group C). All rats had continuous injection for three times, serum IL-2, IL-4 and IL-10 and whole brain IL-1β level, hippocampal neuronal apoptosis level were measured. The cognitive ability in rats was tested by water maze. Results Water maze test showed on the 1st d, the maze test latency of the control group, the experimental group B and the experimental group C water were decreased gradually; Compared with the control group after 3 days, the latency of the experimental group A, experimental group B and experimental group C were all decreased, the crossing circle times were also reduced. Hippocampal neuron apoptosis were (2.3±1.7)%, (14.7±6.9)%, (4.2±3.3)%, (10.2±4.8)% in control group, experimental group A, experimental group B and experimental group C, respectively. The neurons apoptosis of experimental group A was significantly increased. The serum IL-4 and IL-10 of the experimental group A, experimental group B and experimental group C after anesthesia were significantly higher than the control group. The whole brain IL-1β of the experimental group A, experimental group B and experimental group C were significantly lower than the control group. Conclusions Propofol can reduce anesthesia effect of ketamine on the cognitive function and immune function in the young rats.

417-420

Authors: Yuan-Lu Huang, Xiao-Li Liu, Na Xu, Ya-Juan Xiao, Guan-Lun Gao

Abstract Objective To investigate the expression and function of chemokine receptor CXCR2 and CXCR7 in the rat with acute leukemia. Methods Flow cytometry and RT-PCR were used to detect the CXCR2, CXCR7 expression on the bone marrow cell surface of the acute leukemia group and the control group. Results The bone marrow cell surface CXCR2, CXCR7 relative fluorescence intensity of the observation group was significantly higher than the control group (P<0.05). The CXCR7 expression of the extramedullary infiltration group was significantly higher than non-extramedullary infiltration group (P<0.05). The CXCR2, CXCR7mRNA median expression level of the observation group was higher than the control group. The CXCR2 expression and CXCR7 expression of the observation group was positively correlated, and the correlation coefficient was 0.782 (P<0.01). Conclusions The chemokine receptor CXCR2 and CXCR7 are highly expressed in acute leukemia, which may be associated with the occurrence of leukemia.