Journal of Pharmaceutical Research

57-60

Authors: N. Narayanan, R. Gowri, A. Maheswaran, G. Harshapriya, N. Deattu, N. R. Ravi Nargis

Purpose: WHO estimates that more than 346 million people worldwide have diabetes. Majority of the population today is not just trying to be economically successful but also aim to stay fit and free from dreaded chronic diseases. This investigation aims to evaluate the health conditions of people from remote areas who have less possibility of utilizing the urban health facilities of India. Methodology: A cross sectional study was conducted to clinically assess diabetes in rural population ofThiruvaliurdistrict by using a predesigned and pretested protocol to find out the prevalence and the risk of diabetes mellitus in general population. The study mainly focuses on rural population so as to suggest possible treatment and remedial aspects. Various studies in the test used are based on simple anthropometric, demographic and behavioral factors to detect undiagnosed diabetes. The responses for the questions were collected, evaluated and statistically analyzed. Findings: Out of 250 subjects, only 5% showed symptoms of diabetes (first time diagnosis) and among them half were in the early stage of the disorder. Conclusion: This study reveals that diabetes awareness prevailed among selected individuals about the hygienic system and life style. It was found that people acquired maximum knowledge with better understanding of the disease, better impact on the progression ofthe disease and complications by patient counseling through verbal and pictograms.

66-70

Authors: Malathi Raghunath, Amol Dhamne

Background: Ciprofloxacin Hydrochloride (CPX) is a fluoroquinolone antibacterial. Tinidazole (TNZ) is a nitroimidazole anti protozoal while dicyclomine hydrochloride (DIC) is an anticholinergic antispasmodic agent. The three drugs in their fixed dose combination are frequently administered in typhoid fever and infections of mixed origin. Purpose: The aim of the present research was to develop a reversed-phase high-performance liquid chromatography method for simultaneous estimation of CPX, TNZ and DIC in bulk and combined tablet formulation. Methodology: HPLC system used was Thermo Finnigan coupled to a variable wavelength UV detector and was operated in isocratic mode. The data acquisition was carried out using Chrome Quist software. The separation was achieved using HiQ Sil C18 column having dimensions 250 x 4.6 mm i.d. with a particle size of 5 ~m. The mobile phase consisted of buffer pH 4.0 and methanol combined in ratio of 60:40 v/v. The flow rate and UV detector was set at 1 ml/min and 218 nm respectively. Findings: The order of elution of all three drugs was found to be TNZ (5.42 min), DIC (6.96 min) and CPX (8.04 min). The linearity was established in the range of 5-30 ~g/ml (CPX and TNZ) and 250-650 ~g/ml (DIC). The method was validated in accordance with ICH guidelines. Conclusion: From this study it was concluded that the proposed RP-HPLC method is accurate, reproducible and precise. Application: The developed method was then successfully applied for simultaneous estimation of these drugs in marketed tablet dosage form.