European Journal of Molecular Biotechnology

3-7

Authors: Magda D. Davitashvili, Gela S. Azikuri

The investigation is concerned with the detection of the rII region in the phages, related to T-even, by means of some complementation and recombination tests with deletion mutant of phage T4BrII-1272. The restoration of the damaged function and the recombinants were detected by planting the progeny of reference and mutant strains of bacteria. The analysis of the results obtained shows the ability of five phages out of the eight to restore the damaged function in deletion mutant of phage T4BrII-1272 (Zonne 2, Zonne 3 and Zonne 4 phages were not complement). The formation of recombinants was noted only with DDVI and Zonne 7 phages. The percentage of recombinants was 0.004 and 0.2 respectively.

17-24

Authors: Pavel А. Krylov, Nikita I. Stepanenko, Natalia A. Borozdina

The article is concerned with lungs proteome analysis of live-stock animals (Bos Taurus and Sus scrofa) with further proteins grouping by their physico-chemical and functional properties. Primary information of proteins comprised in the lungs proteomes of Bos Taurus and Sus scrofa was obtained from the UniProt database, taking into account the functional properties received from Gene Ontology database. The analysis revealed several thousand annotated proteins used for further grouping: by the chemical nature of their prosthetic groups, their localization in relation to the cell and functional properties. Consequently, we found a predominance of phosphoproteins in the lungs proteome, exceeding by almost 2 and 10 times the number of proteins related to glycoproteins and lipoproteins, respectively. Protein analysis by their localization in relation to the cell reveals a predominance of membrane and intracellular proteins. Practically significant proteins were extracellular proteins. Maximum function diversity of proteins comprised in the lungs proteome was in Bos Taurus - 741, Sus scrofa – 379. Therefore, lungs proteins of Bos Taurus are more promising for the industrial production than Sus Scrofa’s ones. The obtained data can be used as a basis for the development or optimization of protein isolation methods for the pharmaceutical and biotechnology industries demands in future.

40-46

Authors: Valery G. Zaitsev, Regina Yu. Ivashchenko, Darya A. Kurkina, Anna S. Popova

Development and growing of plant cultivars with elevated content of health beneficial nutrients could improve the public human health. The aim of this study was to review the state of the art in use of molecular markers to produce anthocyanin-rich varieties of lettuce (Lactuca sativa L.). Link between anthocyanins production and their proved effect on the health was discussed. Although anthocyanin-dependent red colour of lettuce has been used in breeding for years the metabolic markers are not high-quality for noted purpose. Use of molecular markers can improve results of the breeding. Known and perspective DNA-based molecular markers were shown in this mini-review.