European Journal of Molecular Biotechnology

Number 5 (2), 2017

52-59
Synthesis, spectroscopic NMR and theoretical (HF and DFT) investigation of 3,5,5,9-tetramethyl-2-nitro-6,7,8,9-tetrahydro-5H-benzocycloheptene and 2,5,9,9-tetramethyl-1,3-dinitro-6,7,8,9-tetrahydro-5H-benzocycloheptene

Authors: N. Ourhriss, A. Zeroual, C. A. Gadhi, A. Benharref, A. Abourriche, A. Bennamara, A. ElHajbi

Abstract

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3,5,5,9-tetramethyl-2-nitro-6,7,8,9-tetrahydro-5H-benzocycloheptene and 2,5,9,9-tetramethyl-1,3-dinitro-6,7,8,9-tetrahydro-5H-benzocycloheptene has been synthesized using nitration reaction of ar-himachalene. Also, optimized geometry of the title compound are evaluated using HF and DFT/B3LYP with 6-31(d) basis set 6-31G(d) methods. Moreover, the 13C NMR chemical shift values of the molecule (2,5,9,9-tetramethyl-1,3-dinitro-6,7,8,9-tetrahydro-5H-benzocycloheptene) are calculated using HF and DFT/B3LYP/6-31G(d) and compared with experimental results. The global of the reactivity indices and Parr functions of the reagents were calculated by HF and DFT to identify the actives regions of these molecules, we found that these reactions are regiospecific.

60-65
Extraction of Alkaloids from Three Nigerian Plants, Kola Acuminata (OJI IGBO) Vera Kola (OJI Hausa) and Garcinia Kola (BITTER KOLA)

Authors: I. A. A. Ibrahim, M. M. Mohammad, A. A. Faisal, H. Musa, G. Altine, A. Bununu

Abstract

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Number of views: 681

The extraction of alkaloid from Garcinia Kola (bitter kola) Kola Accuminata (Oji Igbo) and Kola Vera (Oji Hausa) were carried out using 10 % enthanoic acid and 10 % methanol to separate the alkaloid from the residue. The alkaloid was extracted using ammonium hydroxide from the sample results obtained on alkaloid for the sample 9.16 % Oji Igbo, 6.20 % Oji Hausa and 8.20 % Bitter Kola. It was observed that the percentage of alkaloids was highest in Oji Igbo and the least for Oji Hausa. The extracts all exhibited antimicrobial activity against he tested organisms. These activities decreasing with a decrease in extract concentrations. The results suggest the usefulness of thee nuts in the treatment or management of microbial infections without the dangers of side effects from synthetic drugs.

66-73
The QseB/QseC Signaling Affects Initiation of Chromosomal Replication through Regulating Expression of the DnaA Protein in E.coli

Authors: Baigalmaa Luvsandorj, Morigen

Abstract

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The Escherichia coli two-component system, QseB/QseC signaling regulates expressions of more than 50 genes encoding flagellar proteins and proteins associated with virulence. In this work, we found that absence of the qseB gene led to an early initiation of chromosomal replication in a manner of medium-independent, the early initiation was shown to be reversed by ectopic expression of QseB from a plasmid pqseB. Further, absence of the qseB gene resulted in an increase of DnaA concentration and the purified QseB protein did not interact with the oriC DNA in vitro. The early initiation was also found in the ∆qseC mutant cells. These results indicate that absence of the QseB/QseC signaling increases concentration of DnaA and the later subsequently triggers the early initiation of replication. Flourescene microscope analysis showed that the QseC protein localized at cell membrane while QseB was in cytosol.

74-79
Phytochemical Analysis and Antibacterial Assay of Stem Bark of Anogeissus Leiogarpus

Authors: I.A.A. Ibrahim, M. M. Mohammad, A. A. Faisal, H. Musa, G. Altine, A. Bununu

Abstract

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Number of views: 454

Anogeissus leiocarpus is extensively used in Nupe traditional and folklore medicine to cure various human ailments. The preliminary phytochemical screening of the stem bark revealed the presence of flavonoids, soponins, steroids, terpeniods, cardiac glycosides and anthraquinones. In vitro antibacterial studies of the methanol and water extracts of the plant part were carried out on medically important bacterial strains including, Lacbacillus spp, Staphylococcus aureus, Pseudomonas aerugininosa, Escherichia coli, Bacillus spp, and Salmonella typhii using agar plug techniques. The results compared well with standard antibiotics (streptomycin) with susceptibly increasing with concentration. The extracts of the plant showed antibacterial activity, justifying their continued used in treatment of bacterial infections.

80-88
Autopoiesis Concepts for Chemical Origins of Life and Synthetic Biology Stenogram of the popular lecture on the foreign bibliographic seminar

Authors: Oleg V. Gradov

Abstract

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Number of views: 432

The monograph (Luisi P.L. "The Emergence of Life: From Chemical Origins to Synthetic Biology", 2010, Cambridge University Press, Cambridge, New York etc., 315 p.) is a well-written, informative book providing a novel view on the interrelation between the abiogenesis as the natural origin of life and synthetic biology as the artificial synthesis of life. This concept is specially known as autopoiesis. As its name implies, it is a correlate of self-organization, but this word has quite a broad meaning in the literature. Consequently, some further restriction is required for this term in abiogenetic, as well as in "biogenetic" applications. There is, in fact, one basic reason for considering the abiogenetic problem in terms of self-organization theory. It follows from the extremely boundless complexity of biological systems.

Number 5 (1), 2017

4-10
A High-Throughput PCR-Amplification of GC-Rich DNA Sequences

Authors: Alexey S. Afoshin, Philipp V. Kochetkov, Zhanna I. Andreeva-Kovalevskaya, Zhanna I. Budarina, Marina V. Zakharova, Alexander V. Lisov, Andrey M. Shadrin, Alexey A. Leontievsky

Abstract

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Number of views: 574

The PCR amplification of DNA molecules with a high GC content is a complex procedure and often requires optimisation. In last few years several improvements have been developed to facilitate the optimisation of PCR steps. In this work, five high-fidelity commercial DNA-polymerases for the amplification of GC-rich genes were tested. Using the most effective DNA polymerase, we were able to PCR amplify 161 of 187 (87 %) of target genes with a GC content from 57 to 77 % mostly up to 2500 bp long without an optimisation steps. The final yield of specific PCR products was more than 150 ng from 100 µL PCR reactions mix after the purification of specific DNA products by agarose gel electrophoresis.

11-22
Distribution of Molecules of ZEOLITH detox and ZEOLITH Crème in Water as Factor for Health

Authors: Ignat Ignatov

Abstract

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Number of views: 486

We studied the mathematical model of interaction with water of natural mineral and microporous crystalline mineral ZEOLITH detox and ZEOLITH Creme of LavaVitae Company (Austria). In this report are submitted data about the interaction of ZEOLITH detox and ZEOLITH Creme with water, obtained by non-equilibrium (NES) and differential-equilibrium energy spectrum (DNES) of water. The average energy (∆EH... O) of hydrogen Н…O-bonds among individual molecules H2O after treatment of ZEOLITH detox with water measured by NES- and DNES-methods is ∆E=-0.0034±0.0011 eV for ZEOLITH detox. The average energy (∆EH... O) of hydrogen Н…O-bonds among individual molecules H2O after treatment of ZEOLITH detox with water measured by NES- and DNES-methods is ∆E=-0.0034±0.0011 eV for ZEOLITH detox. The average energy (∆EH... O) of hydrogen Н…O-bonds among individual molecules H2O after treatment of ZEOLITH detox with water measured by NES- and DNES-methods is ∆E=-0.007±0.0011 eV for ZEOLITH Creme. These results suggest the restructuring of ∆EH... O values among H2O molecules with a statistically reliable increase of local extremums in DNES-spectra. The research is performed for ZEOLITH detox with study of pH and oxidative reduction potential (ORP).

23-29
Processes in Catholyte and Anolyte as Result of Water Electrolysis

Authors: Dimitar Mehandjiev, Ignat Ignatov, Stoil Karadzhov, Georgi Gluhchev, Atanas Atanasov

Abstract

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Number of views: 525

A two stage model of the physicochemical processes at the electrolysis of pure water is proposed. The presence of nascent hydrogen in the catholyte and nascent oxygen in the anolyte during the first stage explains the antioxidant properties of the catholyte and the strong biocidal action of the anolyte. In the second stage the nascent hydrogen and oxygen are combined into hydrogen and oxygen molecules, respectively. The comparison between their average energies with the average energy of the control sample of water shows an increase in the average energy of the catholyte and decrease in the average energy of the anolyte. This indicates that some changes in the structure of the activated water have occurred.

30-42
Synthesis, Spectral Sensitization, Solvatochromic and Halochromic Evaluation of New Monomethine and Trimethine Cyanine Dyes

Authors: H.A. Shindy, A.K. Khalafalla, M.M. Goma, A.H. Eed

Abstract

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Number of views: 475

New heterocyclic starting material namely 4,5-dimethyl-2,7-diphenyl-furo[(3,2-d), ( -d)] bis pyrazole were prepared and employed to synthesis of some novel monomethine cyanine dyes (simple cyanine dyes) and trimethine cyanine dyes (carbocyanine dyes). Spectral sensitization evaluation for all the synthesized cyanine dyes was carried out through investigating their electronic visible absorption spectra in 95% ethanol solution. Solvatochromic and/or hachromic evaluation for some selected dyes were carried out through examining their electronic visible absorption spectra in pure solvents having different polarities [Water (78.54), Dimethylformamide (36.70), Ethanol (24.3), Chloroform (4.806), Carbontetrachloride (2.238) and Dioxane (2.209)] and/or in aqueous universal buffer solutions having varied pH values (1.75, 2.45, 4.65, 5.80, 7.88, 8.75, 10.85 and 12.60 units), respectively. Structure determination were carried out via elemental analysis, visible spectra. Mass spectrometer, IR and 1H NMR spectral data.

43-49
MEDT Study of the Mechanism and Regioselectivity of Diazocompounds and Alkenes in [3+2] Cycloaddition Reaction

Authors: A. Zeroual, M. El Idrissi, R. El Ajlaoui, N. Ourhriss, S. Abouricha, N. Mazoir, A. Benharref, A. El Hajbi

Abstract

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The mechanism and regioselectivity of diazomethane with 2-methyl-but-2-ene and (diazomethylene)dibenzene with Methylene-trifluoromethyl-phosphane in [3+2] cycloaddition, have been theoretically studied at the DFT/ B3LYP/6-31(d) computational level. The possible ortho/meta regioselective channels were explored and characterized, the energies analysis associated with the different reaction pathways and the analysis of the density map of the transition indicates that the 1,3-DC reaction of diazomethane with 2-methyl-but-2-ene and (diazomethylene)dibenzene with Methylene-trifluoromethyl-phosphane are highly regioselective, in good agreement with the experimental observations.