European Journal of Molecular Biotechnology

52-71

Authors: Ignat Ignatov, Oleg Mosin, Borislav Velikov, Enrico Bauer, Georg Tyminski

In this paper are submitted data on longevity factors and mountain water in factorial research of phenomenon of longevity in mountainious and field areas of Bulgaria. The dependence was established among various internal and external factors on a phenomenon of longevity – residence area, health status, gender and heredity. It was shown that water is among the most important factors for longevity. Natural waters derived from various Bulgarian water springs were investigated by non-equilibrium energy (NES) and differential non-equilibrium energy spectrum of water (DNES) method. The biological effects of water with varrying content of deuterium are also discussed. It was shown, that the increased content of deuterium leads to physiological, morphological and cytology alterations of the cell, and also renders negative influence on cellular metabolism, while deuterium depleted water with reduced deuterium content on 20–30 % has beneficial effects on health. By using IR-spectroscopy were investigated various samples of water with varying contents of deuterium, received from Bulgarian water springs and blood serum of cancer patients as well. As estimation factor was measured the values of the average energy of hydrogen bonds (∆EH...O) among H2O molecules, as well as local maxima in the IR-spectra of various samples of water and human blood serum at -0,1387 eV and wavelength 8,95 μm. For a group of people in critical condition of life and patients with malignant tumors the greatest values of local maxima in IR-spectra are shifted to lower energies relative to the control group. This testifies to the structural changes of water. The obtained results testify to necessity of consumption of clean natural water which quality satisfies mountain water from Bulgarian water springs.

86-94

Authors: Alexander A. Shiroky, Alexander V. Volkov, Valery V. Novochadov

The article is an attempt to explain the renewal of the articular cartilage in normalcy and osteoarthritis development by principles of mathematical modeling. Such models help to develop advanced methods of prevention, detection and treatment of osteoarthritis including molecular biotechnologies based on tissue engineering conception. We used histological images to perform structural analysis to discover the signs of active system and its states. Received data are useful to develop research protocols in cartilage tissue engineering.