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Research of expression quantity of serum miR-146a and miR-146b in patients with acute cerebral infarction before and after the intervention of rosuvastatin
Authors: Xiao-Yan Zhu, Rong-Yao Hou, Shun-Chang Sun, Xi-Yan Zhou
Number of views: 424
Objective: To study the expression quantity of serum miR-146a and miR-146b in patients with
acute cerebral infarction before and after the intervention of rosuvastatin and its correlation
with toll-like receptor 2 (TLR2) and TLR4 signaling pathways.
Methods: A total of 65 patients with acute cerebral infarction treated in our hospital from
December 2015 to August 2016 were selected for prospective study. They were treated with
lipid-lowering rosuvastatin, and peripheral blood samples were collected at 8th week before
and after treatment, respectively. Serum was separated and expression quantity of miR-146a
and miR-146b and contents of TNF-α, interleukin (IL)-1β, IL-6 and IL-17 were determined.
Peripheral blood mononuclear cells were isolated and fluorescence intensities of TLR2, TLR4,
myeloid differentiation primary response gene 88 (MyD88), interleukin-1 receptor-associated
kinase 1 (IRAK-1) and nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB)
were measured.
Results: At 8th week of intervention of rosuvastatin, expression quantity of serum miR-146a
[(0.762 ± 0.092) vs. (0.346 ± 0.053)] and miR-146b [(0.714 ± 0.088) vs. (0.317 ± 0.047)]
in patients with acute cerebral infarction was significantly higher than those before the
intervention. Fluorescence intensities of peripheral blood mononuclear cells such as TLR2
[(10.34 ± 1.27) vs. (16.94 ± 1.94)], TLR4 [(11.37 ± 1.54) vs. (24.35 ± 3.26)], IRAK [(9.34 ±
0.92) vs. (15.32 ± 1.82)], MyD88 [(4.42 ± 0.56) vs. (9.41 ± 1.03)] and NF-kB [(6.65 ± 0.78)
vs. (13.49 ± 1.76)] and contents of inflammatory factors such as TNF-α [(64.26 ± 8.29) μg/L
vs. (106.39 ± 13.84) μg/L], IL-1β [(37.91 ± 5.24) μg/L vs. (64.23 ± 8.33) μg/L], IL-6 [(34.28
± 4.85) ng/L vs. (82.46 ± 11.97) ng/L] and IL-17 [(56.75 ± 7.49) ng/L vs. (98.31 ± 11.36) ng/
L] of serum were all significantly lower than those before the intervention. Expression quantity
of serum miR-146a and miR-146b had a negative correlation with fluorescence intensities
of TLR2, TLR4, IRAK, MyD88 and NF-kB. Fluorescence intensities of TLR2 and TLR4 in
peripheral blood mononuclear cells had a positive correlation with contents of TNF-α, IL-1β,
IL-6 and IL-17 in serum.
Conclusions: Treatment with rosuvastatin can up-regulate the expression quantity of serum
miR-146a and miR-146b in patients with acute cerebral infarction and further inhibit the
secretion of IRAK, MyD88, NF-kB, TNF-α, IL-1β, IL-6 and IL-17 mediated by TLR2 and
TLR4.