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The effects of different strength of MS media in solid and liquid media on in vitro growth of Typhonium flagelliforme
Authors: Nur Inani Rezali, Norrizah Jaafar Sidik, Azani Saleh, Nurul Izzati Osman, Nurul Athirah Mohd Adam
Number of views: 609
Objective: To determine the effects of different strength of Murashige and Skoog (MS)
media (full, 1/2 and 1/4) in solid and liquid media on in vitro growth of Typhonium
flagelliforme (T. flagelliforme), whereby an optimum media composition can be provided
for mass propagation of T. flagelliforme.
Methods: Rhizome bud of T. flagelliforme was obtained from the axenic in vitro
established T. flagelliforme plantlets in Plant Tissue Culture Laboratory, Universiti
Teknologi MARA, Shah Alam. Rhizome bud was used as explant and cultured onto shoot
proliferation medium under different strength of MS media (full, 1/2, 1/4) in solid and
liquid culture media.
Results: After 6 weeks of culture, the number of shoot, number of leaf, number of root,
height of shoot, fresh weight, dry weight and chlorophyll content of T. flagelliforme were
analyzed. A comparison was made between liquid and solid culture media. The results
revealed that the liquid culture media were more effective for all the growth parameters
(shoot height, shoot number, leaf number, root number, fresh weight, dry weight, chlorophyll a and chlorophyll b content) compared to solid culture media. Apart from that,
this study revealed the positive relationship between strength of MS media and type of
culture media (solid and liquid media) to the growth of T. flagelliforme. Growth of
T. flagelliforme was improved when MS strength was increased in liquid media. In
contrast, growth of T. flagelliforme was improved when MS strength was decreased in
solid media.
Conclusions: Through this study, an optimum media composition for mass propagation
of T. flagelliforme had been established by observing effects of MS media strength and
type of culture media (solid and liquid media) on the growth of T. flagelliforme.