568–573
Immunopotentiality of Ayur vedic polyherbal fo r mulations “ Sar ibadi” and “ Anantamul Salsa” with augmentation of IgM production and lymphocytes proliferation: A preliminar y study
Authors: Md. Moklesur Rahman Sarker, Long Chiau Ming, Md. Zaidul Islam Sarker, M Shahabuddin Kabir Choudhuri
Number of views: 279
Objective: To assess the immunopotentiality of Ayurvedic polyherbal preparations,
“Saribadi” and “Anantamul Salsa”.
Methods: Freshly prepared BALB/c mice splenocytes were cultured with “Saribadi” or
“Anantamul Salsa” treatment [doses of 0.25%, 0.50%, 0.75%, 1.00%, 1.50%, 2.00%,
3.00% and 4.00% (v/v)] at 37 C for 5 days. The immunoglobulin M (IgM) production and
lymphocytes proliferation were determined by ELISA and MTT methods, respectively.
Endotoxin contamination was assessed by treating the preparations with polymyxin B.
Results: The doses of “Saribadi” [0.25%, 0.50%, 0.75% and 1.00% (v/v)] significantly
increased IgM productions (0.966, 0.728, 0.695 and 0.615 mg/mL vs. control 0.265 mg/mL)
and lymphocytes proliferation [absorbance 0.311, 0.394, 0.372 and 0.334 optical density
(OD) vs. control 0.162 OD]. Similarly, the doses of “Anantamul Salsa” [0.50%, 0.75%,
1.00% and 1.50% (v/v)] promoted IgM productions (0.933, 0.919, 0.917 and 0.892 mg/mL
vs. control 0.502 mg/mL) and the doses of “Anantamul Salsa” [0.50%, 0.75%, 1.00%,
1.50%, 2.00%, and 3.00% (v/v)] stimulated lymphocytes proliferation (absorbance 0.395,
0.326, 0.440, 0.398, 0.452 and 0.355 OD vs. control 0.199 OD). The activity of “Saribadi”
and “Anantamul Salsa” was not retarded by the treatment of preparations with polymyxin B.
Conclusions: Immunomodulatory activity of “Saribadi” and “Anantamul Salsa” was
unveiled for the first time. “Saribadi” and “Anantamul Salsa” possess immunostimulating
potential acting through the induction of lymphocyte proliferation and IgM production.
These preparations may be useful in strengthening immune responses. However, further
cellular and in vivo studies are required.