212-216
Biochemical characters and antibiotic susceptibility of Staphylococcus aureus isolates
Authors: Subhankari Prasad Chakraborty, Santanu Kar Mahapatra, Somenath Roy
Number of views: 312
Objective: To observe the biochemical characters and antibiotic susceptibility of isolated
Staphylococcus aureus (S. auerus) strains against some conventional and traditional antibiotics.
Methods: Thirty post operative pathogenic isolated S. aureus strains were used in this study.
Bacterial culture was done in Mueller-Hinton broth at 37 °C. Characters of these strains were
determined by traditional biochemical tests such as hydrolysis test of gelatin, urea, galactose,
starch and protein, and fermentation of lactose and sucrose. Antibiotic susceptibility were carried
out by minimum inhibitory concentration test, minium bactericidal concentration test, disc agar
diffusion test and brain heart infusion oxacillin screening agar. Results: From this study, it
was observed that 100% S. aureus isolates showed positive results in gelatin, urea and galactose
hydrolysis test, 50% isolates were positive in starch hydrolysis test, 35% in protein hydrolysis test,
100% isolates in lactose fermenting test, but no isolate was positive in sucrose fermenting test.
Antibiotic susceptibility testing suggested that 20% of isolates were resistant to kanamycin and
46.67% were resistant to oxacillin. Conclusions: These findings show that all these isolates have
gelatin, urea, galactose hydrolysis and lactose fermenting activity. 20% of these isolates were
resistant to kanamycin and 46.67% were resistant to oxacillin.