FERUM AND CUPRUM CONTENT IN TISSUES OF DIFFERENT ORGANS OF RATS INTOXICATED BY CHLORPYRIFOS
Authors: Y. T. Salyha, Ye. O. Dzen, I. V. Luchka
Number of views: 270
trichlor-2-pyridylphospfothioate, C 9 H 11 Cl 3 NO 3 PS)
is one of the most widely used organophosphate
pesticides worldwide. Like other
organophosphates, it causes inhibition of the
acethylcholinesterase — an enzyme, which
function is to degrade acethylcholine, a
neuromediator activating cholinergic neurons.
However, chlorpyrifos toxicity is not limited by
this mechanism, but can also be mediated by other
metabolic pathways. In animal tissues specific
changes can occur, manifested in increased or
decreased content of some essential elements,
The aim of the study was to investigate
changes in iron and copper concentrations in rat brain, myocardium, liver, and kidney tissues after
1, 3, 6, and 10 days after single chlorpyrifos
application at 30 mg/kg body weight.
Adult male Wistar rats were studied.
Animals were housed under standard vivarium
conditions with 12-hour dark/light period and
unlimited access to water and food. Studied
metals content in animal tissues was determined
by atomic absorption spectrophotometry.
We found a tissue-specific and experiment
period-dependent effects of chlorpyrifos exposure
on iron and copper content in the studied organs.
The largest iron accumulation was observed in
the brain, liver, and kidney tissues of intoxicated
animals, whereas myocardial tissue level was
lower than in control group.
It was shown that chlorpyrifos
intoxication caused a decrease in copper
concentration in myocardium, liver, and kidney
tissues, and almost no effect on brain tissue, and
the most prominent decrease of copper
concentration in these organs was observed on
6th and 10th days after chlorpyrifos exposure.