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Proximate composition, mineral contents, phytochemical constituents, antimicrobial activities and Fourier transforms infrared spectroscopy analysis of bark, stem and seed of Hippophae rhamnoides Linn
Authors: Javid Ali, Bashir Ahmad, Said Hassan, Muhammad Siddique, Farrah Gul, Shafaat Ullah
Number of views: 404
Objective: To compare the proximate composition, mineral contents, antimicrobial,
phytochemical and Fourier transforms infrared (FTIR) spectroscopy analysis of bark, stem and
seed of Hippophae rhamnoides.
Methods: Proximate composition was determined according to the described methods.
Mineral analysis was carried out by atomic absorption spectroscopy and flame photometer.
Antimicrobial activities were evaluated according to the agar well diffusion method.
Phytochemical qualitative analysis was carried out according to the described methods and
functional groups were determined by FTIR Prestige-21 Shimadzu Japan.
Results: The proximate analysis showed high content of protein and fiber in stem and bark.
High content of Na (900 mg/L) and K (670 mg/L) was found in bark powder, while in seed,
high contents of Ca (800 mg/L), Mg (725 mg/L), Fe (250 mg/L) Zn (90 mg/L) and Mn
(65 mg/L) were found compared to stem and bark. Phenols, flavonoids and tannins showed
high contents in stem and bark of all extracts. The bark aqueous extract showed high zone
of inhibition against Staphylococcus aureus (21 mm) and Escherichia coli (20 mm), while
methanol extract of stem showed high zone of inhibition (14 mm and 13 mm) against
Enterococcus faecalis and Escherichia coli respectively. The aqueous extract of bark
documented high zone of inhibition against Aspergillus niger (21 mm) and Aspergillus
parasiticus (20 mm). FTIR spectra revealed the presence of OH, C-O and C=O functional
groups.
Conclusions: The study concludes that bark, stem and seed extracts will be useful guideline
for the new syntheses of feed, food supplements and herb drugs with various combination,
which can be used for the treatment of many diseases at global level especially in tropical
regions as well as the male nutrition problems in these areas.