Journal of Coastal Life Medicine

931-939

Authors: Pattira Kasamesiri, Shettapong Meksumpun, Charumas Meksumpun

Objective: To find the possible way to predict the mode of embryonic development of nthued viberlaignecrhss., focusing on egg mass characteristics, embryonic development, and shell patterns of Methods: Eight species of nudibranch were collected in the eastern part of the Gulf of Thailand. The specimens were allowed to copulate and lay their egg masses under laboratory conditions. Eunmtbilr hyoantcihc idnegv. elopment was monitored under a light microscope with a digital camera every day Results: Most of the species of nudibranch collected had a single larva in each egg capsule tshpaetc idmeveenlso pheadd, tehxec esapmt feo rp aJtoterurnn noaf cfuenlle bdriivsi,s iwohni cahn dh ahda t1c-h4in lga rivnatoe tihne e wacahte er gcgo lcuampnsu dleu.r iAnlgl tthhee veliger form. However, the species developed at different rates in each stage. Conclusions: All species in the current study had planktotrophic development except Doriprismatica atromarginata, which showed lecithotrophic development. Based on embryonic dapevpeelaorpemd eton tb aem thone gm tohset nauddviabn

947-952

Authors: Yun Kyung Shin, Jung Jun Park, Jeong-In Myeong, Mi Ae Jeon, Jung Sick Lee

Objective: To examine the glycogen storage and lipid digestion, as well as detoxification of the digestive gland of the tunicate, Halocynthia roretzi. Methods: Tunicates used in this study were collected from tunicate aquafarm located in Hansan Bay on the southern coast of Korea. Light and electron microscopy was performed. Results: The digestive gland was divided into the tubular and blind ampulla portions. Fine veapcituhoellaira gorfa nthuele bs lwinedre a pmrpesuelnlat , paonrdti olinp.o Efupsicthine lgiaral ncuellelss wofe rteh ec obnlfiinrdm eadm ipnu ltlhae pcoyrttoipolna scmou oldf tbhee classified into three types. Conclusions: The results indicate that these three types of cells are involved in glycogen storage, lipid digestion and detoxification.

962-969

Authors: Mediesse Kengne Francine, Woguia Alice Louise, Fogue Souopgui Pythagore, Atogho-Tiedeu Barbara, Simo Gustave, Thaddée Boudjeko

Objective: To examine the total phenolic and protein contents, and the antioxidant cell wall polysaccharide fractions of Stevia rebaudiana leaves. activities of Methods: Three different polysaccharide-enriched fractions, namely FPE (extract with 50 mmol/ L ethylene diamine tetra acetic acid), FPK (extract with 0.05 mol/L KOH) and FH (extract with 4fr amcotilo/nLs KwOaHs )e vwaelruea teexdt rbaacsteedd ofnro tmhe Sirt eavbiiali trye btoa uscdaiavnenag lee aves. The antioxidant activity of these free radical, to reduce ferric power, to chelate ferrous ion aDnPdP Hto (p1r, o1t-edcti phhuemnyaln- 2-picryl hydrazyl) DNA. Results: The results indicated that protein content was found to be higher in FPK polysaccharide eacncriocrhdiendg ftroa cthtieo nF o(4li7n.-48C iμogc aplteeru mmge tohfo dF PwKa)s. Fhuigrhtheer rimn oFrPeK, t(h1e7 .7p1h μengo fleicru cliocm apcoidu)n. dT haen aDlPyPsiHs maximal inhibition percentage of the three polysaccharide-enriched fractions at 400 μg/mL was 2e7x.h6i6b%it, e5d9 .a9 0f%er raincd r e2d3.u2c1i%n gr epsopwecetri veexlcye pfotr thFeP E, FPK and FH. All the polysaccharide fractions peroxidation inhibition, and they completely rFevHe rotende . thTeh e three fractions also exhibited lipid DNA damage induced by H2O2/FeCl2. FanPdK lsiphiodw peder tohxeid sattrioonng iensht isbciativoenn.g ing activity against the DPPH radical, the best chelating ability Conclusions: Stevia cell wall polysaccharide fractions are potent protective agents against poxoildyastaicvceh satrriedses.s Tfrhaec tainoanlsy.s iHs orweveevaelre,d t hmea 0jo.0r 5d miffoelr/eLn cKeOs Hin p tehcet iann tfiroaxcitdioann t (FacPtKiv) itsyh oinw ethde b tehtrteeer antioxidant activity.

975-979

Authors: Nadjib Mohammed Rahmoun, Hanane Ziane, Zahia Boucherit-Otmani

Objective: To describe the ethnopharmacology study and screening of the antimicrobial activity of hydroalcoholic and chloroform extracts of the four plants Ceratonia siliqua (C. siliqua), Salvadora persica, Aloe vera and Anastatica hierochuntica. Methods: The antimicrobial activity was determined using diffusion disk and solid agar dilution methods against 12 bacteria, according to the recommendations of the Clinical and Laboratory Standards Institute. Results: The ethnopharmacology study provided useful information about how the parts used for tohne tphree ppaorlaatiroitny oof fe xthtrea cstosl. vTehnet euxsteradc.t sT hobet ahiingehde rb yy imeladcse rwateioren rthevoesael evxatrriaabcltes yoibetladisn deedp ebnyd tihneg hinytderroeastlicnogh oalnicd seoxlevretnedts .s iTghneif Aicnaanstt aantitciab ahciteerroicahl uancttiicvait ya n(md iCni. msiulimqu ian heixbtritaocrtys cwoenrcee bnytr faatrio tnh eo fm 0o.0s7t to 0.13 mg/mL). Conclusions: These results suggest that C. siliqua could serve as an alternative source of antibacterial agents for human protection against infectious diseases.

986-992

Authors: Brahmi Fatiha, Madani Khodir, Stévigny Caroline, Chibane Mohamed, Duez Pierre

Objective: To determine the quantitative paremeters of rosmarinic acid in Algerian mints, Mentha spicata L. (M. spicata), Mentha pulegium L. and Mentha rotundifolia (L.) Huds by high ptheersfoer pmlaannct ee xtthrianc tlsa yoenr lcinhorloemica taocgirda ppheryo x(HidPaTtLioCn)-. densitometric method and screen the effects of Methods: The analyses were performed on HPTLC silica gel 60 F254 plates with chloroform: aact e3t6o5n ne:m f oarnmdi cf laucoirde s(7c5e:n1c6.e5 :a8t. 5λ, v/v) as the mobile phase. Rosmarinic acid was determined in UV extracts on linoleic acid peroxidaextci o3n25 w nemre wmietha sau r5e5d0 bnym a fnil itnerd,i rreecstp einc tviivteroly c. oTlhoeri mefefetrcitcs mofe tphloadn.ts Results: Chromatographic resolution permitted reliable quantification in both measurement cmoondtaeisn a snidg nciafilcibarnattliyo nh icguhrevre sc ownecreen ltirnaetiaorn isn oaf rraonsmgea roifn 1i5c0 a-c1i 0d0.0 T nhge/ sdpeont.s iMto.m speitcriact aq uwaans tfiofiucnadti otno faollro Mweedn tthhae sapnpa lyesxitsr aocft sm, a5n.1y samples in a short time with reasonable precision (total precision % and 5.8% for UV and fluorescence detection, respectively). The HPTLC data, allied to assays of linoleic acid peroxidation prevention, suggested the potential of M. spicata (52% Trolox襆 equivalents) as a natural source for inhibitors of lipid peroxidation. Conclusions: Densitometry can be used for routine determination and quality control of rosmarinic acid in herbal and formulations containing

998-1001

Authors: Biswas Neupane, Komal Raj Rijal, Megha Raj Banjara, Basu Dev Pandey

Objective: To ascertain the knowledge and prevention measures against dengue adult population in southern Nepal. among selected Methods: A cross sectional study was conducted in Rapti Zonal Hospital, Dang and Bharatpur rHeogsaprditianlg, tChheiitrw kanno wdluerdigneg aAnudg pursatc-tNicoevse ambboeurt , d2e0n1g1u. eA f etvoetar.l of 212 patients were interviewed investigated for the presence of anti-dengue Serum samples were collected and IgM antibodies by IgM-ELISA. Results: Data from 156 patients greater than 15 years were analyzed. Among them, 21 samples fwaermree frosu, nudn etom ppolossyeesds aanndti -hdoeunsgeuweiv IegsM w aenrteib modoirees fbreyq EuLeInSAtl.y Faefmfeaclteesd, becyo dneonmgiucea.l lOy fa tchtiev eto ptaelo, p1l1e8, (k7n6o%w) nw seyrme pretopmorst eadn dt oh okunsoew d rthaien sd iasneda skei tcdheenng ugea.r dFeenv ewre, rhee kandoawcnh ea sa nthde mmyoasltg cioam wmeoren tbhree emdionsgt tshitee sb eosft mknoosqwuni tporeesv. eGnatirvbea gmee dasisuproessa.l, use of mosquito nets and covering water containers were Conclusions: The overall knowledge of dengue was high, but females, old persons, unemployed sapnedc ihaolu astetewnivtieosn hinad f urteulareti vdeelnyg luiett leed kuncaotwiolend pgreo gorfa dmesn. gPueer.s Tonhse rwefiothre k, nthowesleed ggreo uopf st hme adyi sneeaesde pmroorger afmresq ause an ttloyo lr einp odretnegdu teh per euvseen otifo np.

1007-1008

Authors: Sora Yasri, Viroj Wiwanitkit

Objective: To call for attention that the problem of iodine deficiency and low salt be seen in sea coastal province. quality can still Methods: A total of 225 salt samples from each available marine salt shop in the sea coastal piordoivnine cceo noft eTnht.a iTlhane db awseirce s ccorelleencitnegd .t oAolll wsaams pI-leKsI Tw eterest p(Masasheidd otol Uthnei vlaebrsoirtya,t oTrhy afiolra nthde), awnhailcyhs iws aosf the standard test used in previous survey. Results: Of overall 225 samples, there was no sample that could pass the acceptable criteria of iodine content (100 mg/L). It gave the iodine content failure rate equal to 0%. Conclusions: Intensive research of the iodine content in salt sold and available in a sea coastal apvroavilianbclee offr oTmha milaanridn es hsoawlts f tiheladt tbhuet sfaroltm s otlhde ind isrta lsta slht ofipe lidn ftrhoims s reeam cootaes tparlo pvrinovciensc be eicsa nuoste l oocf atlhlye uhsigeh oefr icoodsint eo ff omrtairfiicnaet isoanlt s. hAosu ald r beseu altp, ptlhieed c oton tarnoyl oafr esaaslt, sqeuaa lciotya sitna la onry n aorne asse ais c sotailslt anle.eded. The