704-713
Phytoconstituents and diuretic activity of Cymbopogon citratus leaf infusions in humans
Authors: Christopher Ekpenyong, Nyebuk Daniel, Ernest Akpan
Number of views: 321
Objective: To assess the diuretic activity of infusions prepared from Cymbopogon citratus (C.
citratus) leaves in healthy volunteers.
Methods: One hundred and five subjects (55 men and 50 women) aged 18 to 35 years were
randomly assigned to groups set to orally receive infusions prepared from 2, 4, or 8 g of C. citratus
leaf powder, once daily for 30 d. Urine volume, frequency of urination, urine specific gravity, and
plasma and urinary levels of electrolytes were assessed 1 day before (baseline), and at 10 and 30 d
after initiation of treatment. Computed diuretic indices were compared between experimental and
baseline values.
Results: Subjects treated with infusions prepared from C. citratus leaf powder which tested
positive for saponins, tannins, flavaniods, phenols, anthraquinones, alkaloids, and deoxysugar exhibited a significant increase in indices of diuresis including urine volume, urination
frequency, diuretic action, natriuretic and saliuretic indices and renal fractional excretion of
electrolytes and metabolic acidosis. A non-significant change in urine specific gravity was
observed in all groups. eGFR showed a non-significant increase at Day 1 0, but decreased
significantly (P<0.05) at Day 30. Thiazide and aldosterone secretion indices decreased at Day 10,
whereas carbonic anhydrase index increased significantly (P<0.05) at both Days 10 and 30.
Conclusions: These results indicate a loop active diuretic action of C. citratus infusion.