Jurnal Ilmu Pertanian Indonesia

73-78

Authors: Rissa Herawati Ginting, Achmad Farajallah, Dyah Perwitasari Farajallah, Aron Batubara

Myostatin gene plays a role in helping to control the growth and development of muscle tissue. Identification of genetic diversity in nine local goat breeds in Indonesia has done. The aim of this study was to obtain information myostatin gene diversity of exon 3 in local goats in Indonesia. The total of 10 samples was selected from 80 samples of goat's blood collected comprising each sample of the population of goats breeds, i.e., Samosir, Muara, Kacang, Costa, Peranakan Etawah, Boerawa, Gembrong, Boer, and Boerka goats. The gene diversity and nucleotide base changes were identified by using polymerase chain reaction (PCR) and sequencing techniques. The analysis showed that there is eight variant identified in appropriate with those found in sequencing results. Deletion variations were found in Costa and Samosir goats in T552- and G560-. Substitution variations were found in Gembrong (A7C & A11T), Peranakan Etawah (T10A & A11T), Burawa (T10A & A11T), Muara (A11T), Samosir (A11T), and Boerka (A182T, T437A, T439A, & A445G). Variations on the chromatogram peak overlapping contained in the base position to 13. Analysis of variance showed that there was a special mutation in exon 3 that affects the amino acid tyrosine into lysine. Variants were found in nine goat breeds associated with phylogenetic and genetic distance of goats, Boerka goat has the highest level of genetic variation, it indicated that Boerka goat was crossbreed

87-91

Authors: Irmanida Batubara, . Husnawati, Latifah Kosim Darusman, Tohru Mitsunaga

ati belanda (Guazuma ulmifolia Lamk) leaves is traditionally used as slimming and cholesterol reducing agent. This research aims to determine the active component on jati belanda leaves as marker compound for cholesterol reducing agent. The quality of leaves was checked prior to use for next step of analysis. Jati belanda leaves with good quality was extracted by ethanol 30% and the extract was separated by chromatography technique such as open column chromatography and preparative high performance liquid chromatography. The results showed that the quality of samples is meet to the requirement of Indonesian Food and Drug Agency (Badan Pengawas Obat dan Makanan (BPOM)) based on moisture content, ash content, and heavy metal content. Based on the separation results showed that the cholesterol reducing compound from jati belanda is quercetin

99-107

Authors: Feriatin

This study aimed to determine the type composition, vegetation structure, and the patterns of utilization to support food security in the sub district of South Wakorumba. This study employed a quadrat method. The results of this research show that the composition of plant species in gardens in South Wakorumba sub district was comprised of 25 families from 44 species of plants. For plants vegetation structure the highest of importance value for trees of the class was indicated by coconut (Cocos nucifera L.) species. Whereas the lowest value was indicated by water apple (Eugenia aquea Burm. F) species. For of poles, the highest of importance value was indicated by chocolate (Theobroma cacao L.) species. Whereas the lowest value was by Jamaica cashew (Syzygium malacensis) species. The class of saplings highest value was indicated by papaya (Carica papaya L.) species. whereas the lowest was indicated by tamarind (Tamarindus indica L.) species. For of seedling the highest of importance value was indicated by coconut (Cocos nucifera L.) species. Whereas the lowest of importance value was indicated by rambutan (Nephelium lappaceum L.) species. The pattern of utilization of garden plants by the villagers of Wakorumba Sub District is as follows: as a source of carbohydrate (18.18%), proteins (11.36%), vitamins (45.45%), minerals (22.73%), and fat (2.27%)

115-126

Authors: Rany Utami, Eka Intan Kumala Putri, Meti Ekayani

Oil palm is one of the major plantation commodity that earns foreign exchange. The expansion of oil palm plantation potentially gives not only economic benefits, but also the environmental problems. Therefore, the economic and environmental impacts of oil palm plantation expansion need to be assessed. This study uses revenue analysis to estimate the economic benefits, descriptive analysis to identify environmental impacts, and to estimate external costs using replacement cost and cost of illness. In this research, expansion is defined as the conversion of a part of or whole rubber plantation to oil palm plantation. The expansion resulted in farmers turning some or all of rubber gardens into palm oil plantations that resulted in an increase in revenues of 40.52%. In addition to the on-farm sector, farmers benefit from off-farm (24.46%) and non-farm (25.61%), bringing the total increase of farmers' income by 33.42%. Based on community perceptions of environmental impacts due to oil palm plantation expansion in the form of reduced quantity of groundwater, water pollution, and decreased animal population. The environmental impact of crude palm oil (CPO) factories is to produce liquid waste from palm oil processing that raises external costs for the community in the form of the replacement cost of clean water and the cost of illness

133-139

Authors: Fournita Agustina, Imron Zahri, Muhammad Yazid, . Yunita

The purpose of this study is to identify the problems faced by agricultural extension workers and to formulate alternative of extension strategies in realizing the application of horticultural GAP by farmers in Bangka Regency. This study was conducted in Bangka Regency of Bangka Belitung Province in February 2014. The data obtained in the field were analyzed using qualitative descriptive analysis and SWOT analysis method. The results of this study indicate that: (1) The problems faced by agricultural extension workers in realizing the application of horticultural GAP by farmers in Kabupaten Bangka are low knowledge and skill about GAP horticulture, the existence of agricultural extension workers that have heavy work load, programme extension does not correspond to the need of farmer horticulture; (2) The strategy of agricultural extension workers in realizing the application of GAP horticulture by farmers in Bangka Regency is to increase the training and visit and demonstration plot (plots) GAP horticulture, applying to the Food Security Agency of Bangka Regency related to the lack of education and learning tools horticulture, increasing knowledge and skills on horticultural GAP. Enhance interaction with universities and research institutions, knowledge and skills based on experience farming horticultural crops farmers as well as keeping exercise routines and visits to horticultural farmers

1-5

Authors: Triwardhani Cahyaningsih, Fachriyan Hasmi Pasaribu, Agustin Indrawati

This experiment aims to produce immunoglobulin Y (IgY) Staphylococcus aureus isolates origin staphylococcosis case in rabbits. Vaccination is done several times using Staphylococcus aureus active in 109 cfu/ml. Injecting the chicken is done four weeks in a row, the first week is done by intravenous injection of S. aureus antigens, the second week of the injection sub cutan S. aureus emulsified with Freund's complete adjuvant, followed by the third and fourth week of the injection of S. aureus antigen emulsified with incomplete Freund's adjuvant. Egg samples were taken after four weeks of the last injection for identification, purification, and determination of specific IgY against S. aureus in egg yolk. IgY specificity qualitatively tests performed by the AGP test (To Gel Precipitation). IgY extraction is done using PEG - Ammonium sulfate, purified IgY concentration calculated by the method of Bradford. The results showed that IgY began to be detected in egg yolk at week six after immunization, with the average levels of IgY is 1.7 mg/ml.

13-19

Authors: Resmayeti Purba, Yuti Giametri

The adoption of a new varieties of rice (VUB) are expected could increase the rice production. The study carried out in District of Serang, Pandeglang, Lebak, and Serang City, where each location is implemented in seven villages, so there are 28 locations. The Study of VUB was held on two cropping seasons, that is cropping season II on 2013 and cropping season I on 2014. The new varieties of rice were introduced is Inpari-13, Inpari-15, Inpari-18, and Inpari-19. Rice yields productivity Inpari-19 7.2 ton/ha, is higher than Inpari-13 (6.62 ton/ha), Inpari-15 (6.69 ton/ha), Inpari-18 (6.59 ton/ha), Ciherang (6,30 ton/ha), and Mekongga (6.4 ton/ha). Based on the technical aspects, shows that Inpari-19 adaptive and can increase the yield productivity of 900 kg/ha compared to Ciherang 800 kg/ha, and Mekongga. From the economical aspect the advantages of farming using Inpari-19 and based on the social aspect, showing that the preference of farmers to Inpari-19 like more than Inpari-13, Inpari-15, and Inpari-18. Based on the technical aspects, economic and social, the Inpari-19 can be used as an alternative choice of lowland rice varieties.

25-33

Authors: Simon Petrus Oktovianus Leatemia, Emmanuel Manangkalangi, Paskalina Theresia Lefaan, Hans Fence Zakeus Peday, Luky Sembel

Benthos macroinvertebrates have been used as bioindicators to assess the waters quality of the stream, but still are very rarely to applied in West Papua streams. Benthos macroinvertebrates can describe the stream water quality so that very well be used as bioindicators, due to each species has a different tolerance to interference organic pollutants in waters. This study aimed to assess the water quality of Nimbai stream using benthos macroinvertebrates tolerance level and several water quality parameters. Data retrieval was conducted in JuneJuly and SeptemberOctober 2012 in seven stations (L1L7) from Nimbai upstream to downstream that connected to Prafi river. The water macroinvertebrates were taken using surber with mesh size 200 μm and then was identified at the family level. At L5 station near the oil palm waste disposal installations was contains oil and fat (<1-3 mg/l), which resulted in dissolved oxygen (DO) and pH is lower than the other stations. Similarly the value of H' is low (0.83), however, the value of C (0.79) was high that dominated by Oligochaeta. Famili biotic index (FBI) value obtained showed that water quality in the L1L4 stations was in good to excellent condition, but the quality at L5 station is classified as very poor. As the distance far away from the sources of pollution in the L5 station, water quality tends to improve as shown in the L6 and L7 stations. The result of this study shows the important benefits of benthos macroinvertebrates as bioindicators for assessing the quality of stream waters, and can be described aquatic environments disturbance to the benthos macroinvertebrates habitats in Nimbai stream.

41-45

Authors: Makhziah, Sukendah, Yonny Koentjoro

Mutation is one of the ways to improve genetic variation in plant breeding so the target traits were more easily obtained. Grains of three varieties of maize (Bisma, Madura local, and Gumarang) were treated with gamma 60cobalt irradiation doses (0, 100, 200, and 300 Gy) before sowing then planted in a split plot randomized block design with three replication, to be evaluated for their morphology and agronomic traits. The Main plot was cultivars and sub plot was gamma radiation rates. There was no interaction between gamma rates and cultivars of maize, but increasing of gamma rates caused decreasing of some traits. Some traits such as plant height and leaves number were decreased significantly when gamma rate increased 100 to 300 Gy as well yield components such as grain weight and grain number per ear. Gamma 200 caused produce more ears than other rates. Bisma exhibited greater performance than Madura and Gumarang both of morphology and agronomic traits.

52-66

Authors: La Ode Alifatri, Sigid Hariyadi, Handoko Adi Susanto

The aims of this research were to analyze the land suitability and carrying capacity for groupers culture. The utilized methods were land suitability analysis and carrying capacity analysis. The results of land suitability analysis showed that the area which is fit very suitable criteria is 38.16 ha (17.13%), fit enough suitable is 118.27 ha (53.11%), and appropriate-conditional suitable is 66.26 ha (29.75%). The results of carrying capacity analysis resulted in an area of 156.43 ha which is very suitable for groupers culture development in KJT systems. This area could accommodate as much as 313 ponds which are equivalent to 626 units or 6.257 KJT boxes.