EFFECT OF A LIPOSOMAL PREPARATION OF VITAMINS A, E AND ORGANIC COMPOUNDS OF TRACE ELEMENTS OF Zn, Se, I ON THE PHYSIOLOGICAL STATE OF BROOD CARP DURING PRESPAWNING PERIOD
Authors: Yu. Zabytivskyi, S. Yurchak, L. Bobyliak, I. Hevkan
Number of views: 260
Purpose. Evaluation of the physiological and biochemical status of the organism of brood carps, which during spawning period were fed with a liposomal preparation with a vitamins A, E and organic compounds of microelements of Zn, Se, І.
Methodology. During a 30-day prespawning period, the experimental group of brood carp received a complex liposomal preparation, which included: vitamin A – 5000 IU/kg, vitamin E – 10 mg/kg, Zn – 15 mg/kg (zinc glutamate), Se – 0.3 mg/kg (commercial preparation «Sel-Plex»), І – 5 mg/kg (experimental preparation «Lipoiodine») and sunflower phospholipid – 100 mg/kg. The liposomal emulsion prepared in an ultrasonic disperser at a frequency 35 Hz. Determinations of activity of ALT, AST, ALP, Chol, Ck, and to content of cholesterol, urea, creatinine, uric acid, total protein, albumin and glucose in serum were carried out in the biochemical analyzer Cobas Mira with the use of test-systems.
Findings. We found a positive effect of the supplement on the integrity of cellular membranes of hepatopancreas and kidney that was evident as a reduction in serum ALT activity. A possible increase in ALP activity by 1.9 time indicates on an increase in phosphoric acid metabolism intensity that was related to the processes of phosphorus accumulation in fish eggs. The liposomal supplement has an effect on the reduction of uric acid content in blood by 16 times compared to the control group that indicated on the intensification of protein metabolism. An accumulation of the necessary quantity of amino acids is one of the drivers of the preparation of egg for embryogenesis.
Originality. First demonstration of the effect of the liposomal preparation with vitamins A, E and organic forms of microelements of Zn, Se, І, which was fed to brood carp during prespawning period on the physiological and biochemical characteristics of serum.
Practical value. The results of the work can be used for the development of prespawning brood fish feeding at fish breeding station working in the field of carp reproduction.
Keywords: brood fish, carp, prespawning period, liposomal preparation, vitamin A, E, microelements Zn, Se, І, enzyme, serum.