The Pharmaceutical and Chemical Journal

6-9

Authors: Manik Sharma, Malik Romana, Jeevan Menaria, Sangeeta Devi, Muzaffar Ahmed Sheikh

In the present investigation we were aimed to investigate the antioxidant potential of various crude extracts of leaves of Solanum nigrum. The extraction was carried out by different solvent such as petroleum ether, ethyl acetate and chloroform. The antioxidant property of these extracts was assessed by 2, 2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging method.

15-22

Authors: Bharat Vala, Mayank Bapna, Jigisha Patel

A simple, fast, accurate and precise reverse phase high performance liquid chromatographic method has been developed for the estimation of Griseofulvin in oral suspension. The chromatographic separation was achieved on Zorbax eclipse plus C8 (250 x 4.6) mm; 5µm particle size column with a gradient mixture of mobile phase containing acetonitrile: ortho-phosphoric acid buffer: methanol (10:50:40 v/v). The injection volume was kept at 20 μl with mobile phase at a flow rate of 1.5 ml/min. The wavelength of detection was kept at 254 nm with column temperature at 25 ºC. The selected chromatographic conditions were found effectively to separate Griseofulvin at 12.84 min with its excipients methylhydroxy benzoate at 4.192 min and propylhydroxy benzoate at 11.021 min. The linearity was obtained in range of 125-1250 μg/ml for Griseofulvin, 7.5-75.01 μg/ml for methylhydroxy benzoate and 0.5-5.01 μg/ml for propylhydroxy benzoate with correlation coefficient 0.999, 0.999 and 0.999 for GRS, methylhydroxy benzoate and propylhydroxy benzoate, respectively. The proposed methods were validated as per ICH guidelines and successfully applied for the determination of investigated drugs in oral suspension.