Indonesian Journal of Animal and Veterinary Sciences

81 - 90

Authors: Ali Arsyadi, Abdullah Luki, Karti PDMH, Chozin MA, Astuti DA

This study was carried out to determine the productivity, competition indices and nutrient content of Brachiaria decumbens (grass), Centrosema pubescens and Clitoria ternatea (legumes) as sole and mixed cropping system in peatland in Pekanbaru, Indonesia from October 2011 to November 2012. The experiment was set up in randomized complete block design with five treatments and three blocks as replication. Five treatments compared: B. decumbens sole cropping (Bd), C. pubescens sole cropping (Cp), C. ternatea sole cropping (Ct), B..decumbens and C. pubescens mixed cropping (Bd+Cp) and B. decumbens and C..ternatea mixed cropping (Bd+Ct). The dry matter (DM) yield of B. decumbens was significantly (P<0.05) increased by mixed cropping. B. decumbens DM yield in C. pubescens intercrop increased by 147.9% and in C. ternatea intercrop increased by 74.1% compare to sole B. decumbens. Land equivalent ratio (LER) value range from 1.04 (Bd+Ct) to 1.58 (Bd+Cp). The crowding coefficient (K) value of B. decumbens in both mixed croping system was higher than K value of C. pubescens and C. ternatea. The total K value for Bd+Cp was higher than Bd+Ct. The competition ratio (CR) value of B. decumbens mixed cropping with C. pubescens and C. ternatea were.>1. The aggressivity (A) value of B. decumbens in both mixed cropping was positive. The crude protein (CP) content of B.decumbens did not significantly (P>0.05) increased by mixed cropping with legumes. Intercropping with B. decumbens significantly (P<0.05) decreased CP content of C. ternatea. Meanwhile neutral detergent fibre (NDF) and acid detergent fibre (ADF) content of B. decumbens did not decrease by intercropping with C.pubescens and C. ternatea. In conclusion, mixed cropping with C. pubescens and C. ternatea in peatland increased DM yield of B. decumbens. Mixed cropping with B. decumbens did not influence DM yield of C. pubescens and decreased DM yield and CP content of C..ternatea. Mixed cropping of B. decumbens with C. pubescens and B. decumbens with C..ternatea in peatland did not increase total DM yield of forage per unit area of land and nutrition contents of forage. B. decumbens was more competitive and dominant than C..pubescens and C. ternatea in peatland. Key Words: Cropping System, Forage, Nutrient Contents, Yield, Peatland

104 - 111

Authors: Irma ., Cece Sumantri, Triana Susanti

Prolactin gene plays crucial role in the reproduction and egg production of birds. The objectives of this study were to characterize single nucleotide polymorphism in partial intron and coding region of duck prolactin gene. Blood samples were collected from 168 ducks consisted of 19 Pekin, 36 Mojosari, and 113 of their crossbreds collected from Indonesian Research Institute for Animal Production (IRIAP). Primer pairs for the coding regions in prolactin gene were self designed based on the duck genomic sequence database (GeneBank: AB158611.1). PCR products based on DNA of prolactin gene exon two was amplified approximately 400 bp. There is one base insertion of Adenin at the position of 2001 bp intron two region of duck prolactin. Homology test based on BLAST method indicated 99% identity with duck refference (Code Access GeneBank: AB158611.1). Adenin composition in all of duck samples was higher than refference. Triplet hydrogen bonds between Guanine and Cytosin pairs was higher than those at duplet hydrogen bonds between Adenine and Thymine. All duck samples were homozigous and monomorphyc. Key Words: Prolactin, Mojosari, Pekin, Crossbred

123 - 129

Authors: Ida Zahidah Irfan, A Esfandiari, C Choliq

Determination of serum total protein concentration and main fractions (albumin and globulin) can be used as an important diagnostic tool in clinical biochemistry. Several factors can affect the concentration of total protein, albumin, globulin and albumin globulin ratio (A/G). The aim of this study is to obtain serum protein profiles, albumin, globulin and A/G ratio based on breed, age and BCS (body condition score). Blood samples from 160 bulls were collected. Blood chemistry were analyzed by photometer principle using a commercial kit. There were significant (P<0.001) breed variation on total protein, albumin, globulin and albumin globulin ratio. Significant age differences were observed on total protein and albumin concentration (P<0.001), while globulin concentration and A/G ratio were also significant (P<0.05). Amongs groups of BCS, significant difference was verified only in the albumin concentration (P<0.05). The concentration of total proteins, albumins and globulins in the serum of the bulls are higher than standard values for cattle, while A/G ratio is lower. Key Words: Serum Protein Profiles, Bulls, Breed, Age, Body Condition Score

138 - 142

Authors: Iis Yuanita, L Silitonga, Paulini

Ant-plant (Myrmecodia pendans) is a unique plant which is widely used in Central Kalimantan and contains antioxidant flavonoids and tannin. The use of herbs as organic materials may produce good performance and carcass of chicken. The experiment used 40 Ross strain day old chicks which were randomly divided into 5 treatments and 4 replicates. The treatments were T0 (basal diet as a control), T1 (basal diet+0.1% ant-plant), T2 (basal diet+0.2% ant-plant), T3 (basal diet+0.3% ant-plant) and T4 (basal diet+0.4% ant-plant). Data were collected during 35 days to obtain feed intake, body weight gain, feed conversion, final body weight, mortality, carcass percentage and performance index. The data were analyzed for variances based on a Completely Randomized Design and continued with Duncan’s Multiple Range Test for differences. The result showed no significant effect of treatments on the performances, but T1 treatment (containing 0.1% ant-plant) showed good tendency to gain body weight, final body weight, carcass percentage and performance index respectively 0.51%; 0.44%; 0.15%; and 3.09% higher than T0 (control). It also showed good feed efficiency with feed conversion of value 2.43% lower than control. In conlusion, ant-plant as feed additive until 0.4% in broiler diet had no effect to improve broiler performances. Key Words: Broiler, Ant-Plant (Myrmecodia pendans), Feed Additive, Performance

152 - 158

Authors: Risa Indriani, NLPI Dharmayanti

A/Duck/Sukoharjo/Bbvw-1428-9/2012 virus subtipe H5N1 clade 2.3.2 as seed vaccine on local duck. AI H5N1 clade 2.3.2 vaccine containing 256 HAU per dose was formulated using adjuvant ISA 71VG Montanide ™. Six groups of one day old local duck were used in this study. Three groups (10 ducks per group) were vaccinated and 3 groups (9 duck per group) were served control. Vaccination was conducted when the duck were three weeks old of age using single dose. Three weeks after vaccination when the duck were challenged either with HPAI H5N1 clade 2.3.2, or HPAI H5N1 clade 2.1.3 virus at dose 106 EID50/ 0.1 ml by drops intranasaly. Result showed that vaccination produced 100% protection compared to unvaccinated ducks againt HPAI subtipe H5N1 clade 2.3.2, and 100% protection againt HPAI H5N1 clade 2.1.3 (A/ck/wj/Subang-29/2007 and A/ck/wj/Smi-Part/2006), while unvaccinated ducks showed virus shedding on day 3 post infection. Key Words: Duck, Influenza, Clade 2.3.2, Vaccine, Clade 2.1.3